Identifikasi Escherichia coli dan Salmonella spp pada Karkas Sapi di Rumah Potong Hewan di Banyuwangi dan Resistensi Terhadap Antibiotika

Faisal Fikri(1*), Muhammad Thohawi Elziyad Purnama(2), Amung Logam Saputro(3), Iwan Sahrial Hamid(4)

(1) Fakultas Kedokteran Hewan, Universitas Airlangga, Surabaya
(2) Fakultas Kedokteran Hewan, Universitas Airlangga, Surabaya
(3) Fakultas Kedokteran Hewan, Universitas Airlangga, Surabaya
(4) Fakultas Kedokteran Hewan, Universitas Airlangga, Surabaya
(*) Corresponding Author


Food borne disease can be transmitted through Escherichia coli and Salmonella spp contamination. The contamination of microorganisms with high pathogenic potentials on bovine carcasses results in food borne illness. The aim of this study was to identify multidrug-resistance of Escherichia coli and Salmonella spp on carcass samples that isolated from slaughter house in Banyuwangi. Samples were collected from district of Banyuwangi, Rogojampi, Genteng and Kalibaru. This study used cross sectional study with assumption of prevalence at 50% in each contaminant, confidence level 95% and standart of error at 10%. By the number of samples should reach 96 samples. The result showed that seven samples (7,3%) were positive Escherichia coli and none samples (0%) were positive Salmonella spp. The multidrug-resistance of Escherichia coli showed that Cephalotin (42,9%), Trimethoprim (14,3%) and Erythromycin (42,9%) whereas Ampicillin, Enrofloxacin, Chloramphenicol and Tetracycline were sensitive against Escherichia coli.


Multidrug-resistance; Banyuwangi; bovine carcass; Escherichia coli; Salmonella spp

Full Text:



Ahmad I., Anjum MS., Hanif M. 2012. Prevalence of poultry diseases at high altitudes of district poonch azad jammu & kashmir. Pakistan Journal of Science, 64(4). Anacona JR., Noriega N., Camus J. 2015. Synthesis, characterization and antibacterial activity of a tridentate Schiff base derived from cephalothin and sulfadiazine, and its transition metal complexes. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 137, 16-22. Anil MH., Love S., Helps CR., Habour DA. 2002. Potential for carcass contamination with brain tissue following stunning and slaughter in cattle and sheep. Food Control. 13, 431-436. Badan Standardisasi Nasional. 2008. SNI No. 2897:2008 Tentang Pengujian Cemaran Mikrobia dalam Daging, Telur, dan Susu serta Hasil Olahannya. Badan Standardisasi Nasional, Jakarta. Bai J., Paddock ZD., Shi X., Li S., An B., Nagaraja TG. 2012. Applicability of a multiplex PCR to detect the seven major Shiga toxin–producing Escherichia coli based on genes that code for serogroup-specific O-antigens and major virulence factors in cattle feces. Foodborne pathogens and disease, 9(6), 541-548. Chaudhuri RR., Henderson IR. 2012. The evolution of the Escherichia coli phylogeny. Infection, Genetics and Evolution, 12(2), 214-226. Chen KG., Sikic BI. 2012. Molecular pathways: regulation and therapeutic implications of multidrug resistance. Clinical cancer research, 18(7), 1863-1869. Cheng QF., Sun DW. 2008. Factors affecting the water holding capacity of red meat products: A review of recent research advances. Crit. Rev. Food Sci. Nutr. 48, 137-159. Cheng D., Zhu S., Su Z., Zuo W., Lu H. 2012. Prevalence and isoforms of the pathogenicity island ETT2 among Escherichia coli isolates from colibacillosis in pigs and mastitis in cows. Current microbiology, 64(1), 43-49. Clinical and Laboratory Standards Institute. 2012. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Second Informational Supplement. Clinical and Laboratory Standards Institute, West Valley. Erfianto GI. 2014. Escherichia coli yang Resisten terhadap Antibiotik yang Diisolasi dari Sapi Potong yang Diimpor melalui Pelabuhan Tanjung Priok Jakarta. Fikri F., Hamid IS., Purnama MTE. 2017. Uji Organoleptis, pH, Uji Eber dan Cemaran Bakteri pada Karkas yang Diisolasi dari Kios di Banyuwangi. Jurnal Medik Veteriner, 1(1), 23-27. Fraser TN., Avellaneda AA., Graviss EA., Musher DM. 2012. Acute kidney injury associated with trimethoprim/sulfamethoxazole. Journal of antimicrobial chemotherapy, 67(5), 1271-1277. Gerberding JL., Snider DE., Popovic T., Thacker SB. 2004. Diagnosis and management of food borne illnesses. Morbidity and Mortality Weekly Report (MMWR) 53:4. Guilfoile P., Alcamo IE. 2007. Antibiotic-resistant bacteria. Infobase Publishing. Haileselassie M., Taddele H., Adhana K. and Kalayou S. 2013. Food safety knowledge and practices of abattoir and butchery shops and the microbial profile of meat in Mekelle City, Ethiopia. Asian Pacific J. Trop. Biomed., 3(5), 407-412. Hossain MK., Rahman M., Nahar A., Khair A., Alam MM. 2014. Isolation and identification of diarrheagenic Escherichia coli causing colibacillosis in calf in selective areas of Bangladesh. Bangladesh Journal of Veterinary Medicine, 11(2), 145-149. Hur J., Jawale C., Lee JH. 2012. Antimicrobial resistance of Salmonella isolated from food animals: A review. Food Research International, 45(2), 819-830. Kang HY., Jeong YS., Oh JY., Tae SH., Choi CH., Moon DC., Lee WK., Lee YC., Seol SY., Cho DT. 2005. Characterization of antimicrobial resistance and class 1 integrons found in Escherichia coli isolates from humans and animals in Korea. J. Antimicrob. Chemoth. 55(2005):639-644. Kusumaningsih A. 2010. Beberapa bakteri patogenik penyebab food borne disease pada pangan asal ternak. Wartazoa. 20:103-111. Mandala AY., Swacita IBN., Suada IK. 2016. Penilaian penerapan animal welfare pada proses pemotongan sapi di rumah pemotongan hewan Mambal Kabupaten Badung. Indonesia Medicus Veterinus, 5(1). Pitout JD. 2012. Extraintestinal pathogenic Escherichia coli: an update on antimicrobial resistance, laboratory diagnosis and treatment. Expert review of anti-infective therapy, 10(10), 1165-1176. Popowska M., Rzeczycka M., Miernik A., Krawczyk-Balska A., Walsh F., Duffy B. 2012. Influence of soil use on prevalence of tetracycline, streptomycin, and erythromycin resistance and associated resistance genes. Antimicrobial agents and chemotherapy, 56(3), 1434-1443. Rosano GL., Ceccarelli EA. 2014. Recombinant protein expression in Escherichia coli: advances and challenges. Frontiers in microbiology, 5. Saidi B., Mafirakureva P., Mbanga J. 2012. Antimicrobial resistance of Escherichia coli isolated from chickens with colibacillosis in and around Harare, Zimbabwe. Avian diseases, 57(1), 152-154. Sanchez GV., Master RN., Karlowsky JA., Bordon JM. 2012. In vitro antimicrobial resistance of urinary E. coli among US outpatients from 2000 to 2010. Antimicrobial agents and chemotherapy, AAC-06060. Smith R., & Coast J. 2013. The true cost of antimicrobial resistance. BMJ: British Medical Journal, 346. Sundara I. 2015. Angka lempeng total dan cemaran Escherichia coli pada peralatan pemotongan di tingkat pedagang ayam tradisional Kota Pekanbaru. [Doctoral dissertation] Universitas Islam Negeri Sultan Syarif Kasim Riau. Tao F., Peng Y., Li Y., Chao K., Dhakal S. 2012. Simultaneous determination of tenderness and Escherichia coli contamination of pork using hyperspectral scattering technique. Meat Science, 90(3), 851-857.


Article Metrics

Abstract views : 5367 | views : 3621


  • There are currently no refbacks.

Copyright (c) 2018 Jurnal Sain Veteriner

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Sain Veteriner Indexed by


Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).

Fakultas Kedokteran Hewan, Universitas Gadjah Mada

Jl. Fauna No.2, Karangmalang, Yogyakarta

Phone: 0274-560862

Fax: 0274-560861


HP. 0895363078367

Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.


web stats View My Stats