Deteksi Staphylococcus aureus dan Staphylococcus sp. secara Langsung dari Susu Segar Kambing Peranakan Etawa dengan Polymerase Chain Reaction (PCR)

https://doi.org/10.22146/jsv.60153

Fatkhanuddin Aziz(1*), Fajar Budi Lestari(2), Sarah Nuraida S(3), Endah Purwati(4), Siti Isrina Oktavia Salasia(5)

(1) Program Studi D3 Kesehatan Hewan, Departemen Teknologi Hayati dan Veteriner, Sekolah Vokasi, Universitas Gadjah Mada
(2) Program Studi D3 Kesehatan Hewan, Departemen Teknologi Hayati dan Veteriner, Sekolah Vokasi, Universitas Gadjah Mada
(3) Fakultas Kedokteran Hewan, Universitas Gadjah Mada
(4) Fakultas Kedokteran Hewan, Universitas Gadjah Mada
(5) Departemen Patologi Klinik, Fakultas Kedokteran Hewan, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


The genus of Staphylococcus is one of the bacterial pathogens that cause mastitis causing economic losses in Etawa crossbreed goat. Among Staphylococcus sp. which can grow well in raw milk, Staphylococcus aureus is known causing foodborn disease in human because of its ability to produce enterotoxins that are resistance to digestive enzymes or heating treatment. The purpose of this study was to detect Staphylococcus sp. and S. aureus directly from raw milk of Etawa crossbreed goat using PCR. The study was carried out by extracted DNA from fresh milk using spin column method and then amplified specific 23S rRNA for Staphylococcus sp. and S. aureus. PCR examination revealed that 37 (61%) and 1 (1,6%) raw milk samples were positive for Staphylococcus sp. and S. aureus respectively. The PCR Method is usefull to reduce defection time of Staphylococcus sp. contaminants.

Keywords


Goat; PCR; raw milk; Staphylococcus aureus; Staphylococus sp

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DOI: https://doi.org/10.22146/jsv.60153

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