The Vermicidal Activity of Water Extract Two Types  of Ethnopharmacology Against Haemonchus contortus in Kacang Goat (Capra hircus) In-vitro

I Gusti Komang Oka Wirawan; Suryawati -; Theresia Nur Indah Koni; Redempta Wea

doi:10.22146/jsv.70779

The Vermicidal Activity of Water Extract Two Types of Ethnopharmacology Against Haemonchus contortus in Kacang Goat (Capra hircus) In-vitro

https://doi.org/10.22146/jsv.70779

I Gusti Komang Oka Wirawan^(1*), Suryawati -⁽²⁾, Theresia Nur Indah Koni⁽³⁾, Redempta Wea⁽⁴⁾

(1) Prodi Kesehatan Hewan Politeknik Pertanian Negeri Kupang Kupang
(2) Politeknik Pertanian Negeri Kupang Kupang
(3) Politeknik Pertanian Negeri Kupang Kupang
(4) Politeknik Pertanian Negeri Kupang Kupang
(*) Corresponding Author

Abstract

This research aims at determining the differences in the vermicidal activity between EADMAN and EADMDV in-vitro based on concentration and treatment time. It encompassed three groups of concentrations of EADMAN and EADMDV solution (2.5%, 3.5%, 4.5% from 0.2 g/mL each extract solution), a group of negative control (aqua pro injection), and a group of positive control (Oxfendazole 0.055%). The EADMAN and EADMDV was diluted in 1.5 mL aqua pro injection, then placed on petri dish with a concentration of 2.5%, 3.5%, 4.5% of 0.2 g/mL extract solution. Eight worms placed on each petri dish afterwards. The effectivity of EADMAN and EADMDV was determined based on the percentage of dead worms. The dead and live worms was counted from each immerse group for 1, 3, 5, 7 hours with four replications for each treatment. The vermicidal activity was indicated by touching the worms using a spatula; if it did not react, it was put in warm water. If the worms did not move, it was declared as the dead one, otherwise it was paralysed. Data of effective concentration from both extracts against vermicidal activity was analyzed using a two-way Anova, followed by Tukey test (P>0.05). The result showed the vermicidal activity of 2,5% EADMDV was significantly greater than 4.5% EADMDV, the negative control, and EADMAN at all concentrations. Meanwhile, its difference to 3.5% EADMDV and positive controls revealed insignificant results statistically. Therefore, 2.5% concentration of EADMDV with 7 hours of immersions gave the most effective vermicidal activity against Haemonchus contortus.

Keywords

Parasitology Veterinary

Full Text:

PDF

References

Ahmad, R.Z. dan Gholib, D. 2014. Pemberian Duddingtonia flagrans dan Saccharomyces cerevisiae Meningkatkan Produksi Susu dan Menurunkan Populasi Cacing pada Sapi. Jurnal Veteriner. 15(2): 221-229. Britto, A.J.D., dan Gracelin, D.H. S. 2011. Screening Of A Few Flowers for Their Phytochemical Constituents. Life Sciences Leaflets. 20: 866-871. Dizaj, S.M., Vazifehasl, Zh., Salatin, S., Adibkia, Kh., dan Y. Javadzadeh, Y. 2015. Nanosizing of drugs: Effect on dissolution rate. Research in Pharmaceutical Sciences. 10(2): 95-108 Jain, P., Hossain, K.R., dan Mishu, T.R. 2014. Antioxidant and Antibacterial Activities of Spondias pinnata Kurz. Leaves. European J. of Medicinal Plants. 4(2): 183-195. Jannah, R.N., Sosiawati, S.M., dan Chusniat, S. 2017. Potensi Anthelmintik Ekstrak Biji Mangga Gadung (Mangifera indica L.) Terhadap Cacing Mecistocirrus digitatus Secara in vitro. Journal of Parasite Science. 1(2): 59-64. Joshi, D.R., dan Adhikari, N. 2019. An Overview on Common Organic Solvents and Their Toxicity. Journal of Pharmaceutical Research International. 28(3): 1-18. Matheos, H., Runtuwene, M.R.J., dan Sudewi, S. 2014. Aktivitas Antioksidan Dari Ekstrak Daun Kayu Bulan (Pisonia alba). Pharmacon J. Ilmiah Farmasi – UNSRAT. 3(3): 235-246. Orden, E.A., Rosario, N.A.D., Orden, M.E.M., dan Fujihara, T. 2017. Nutritive Value and Anthelmintic Properties of Selected Leguminous Shrubs and Trees for Goats. The Clsu International Journal of Science & Technology. 2(2): 28 – 37. Qamar, F. dan Maqbool, A. 2012. Biochemical studies and serodiagnosis of haemonchosis in sheep and goats. Journal of Animal and Plant Sciences. 22(1):32-38. Rachmaniar, R., Rusdiana, T., Panatarani, C., dan Joni, I.M. 2017. Review: Usaha Peningkatan Kelarutan dan Laju Disolusi Zat Aktif Farmasi Sukar Larut Air. Indonesian Journal of Pharmaceutical Science and Technology. 6(2): 1-12. Ratnawati, D., Supriyati, R., dan Ispamuji, D. 2013. Aktivitas Anthelmintik Ekstrak Tanaman Putri Malu (Mimosa Pudica l) Terhadap Cacing Gelang Babi (Ascaris suum. L). Prosiding Semirata FMIPA Universitas Lampung. Rollando. 2017. Pengantar Kimia Medisinal. Edisi Pertama. CV. Seribu Bintang. Malang-Jawa Timur. Subronto dan Tjahajati, I. 2008. Ilmu Penyakit Ternak III. Cetakan I. Gadjah Mada University Press. Yogyakarta. Sudjana. 2005. Metoda Statistika. Cetakan Pertama. Tarsito Bandung. Bandung. Widiarso, B.P., Nurcahyo, W., Kurniasih, dan Prastowo, J. 2021. The ultrastructure changes of Haemonchus contortus exposed to bamboo leaves (Gigantochloa apus) aqueous extract under in vitro condition. BIOD I V E R S I T A S. 2(1): 5698-5702. Williams, A.R., Fryganas, C., Ramsay, A., Mueller- Harvey, I., and Thamsborg, S.M. (2014). Direct Anthelmintic Effects of Condensed Tannins from Diverse Plant Sources against Ascaris suum. PLoS One J. 9(5): 1-16. Wirawan, I.G.K.O., Nurcahyo, W., Prastowo, J., dan Kurniasih. 2017. Daya Larvasida Ekstrak Daun Muda Kedondong Hutan terhadap Haemonchus contortus secara in-vitro. Journal Veteriner. 18(2): 283-288. Wirawan, I.G.K.O., Jaya, I.K., dan Randu, M.D.S. 2019. Keragaman dan Intensitas Infeksi Endoparasit Gastrointestinal pada Sapi Bali dengan Sistem Ekstensif di Kabupaten Kupang. Journal Sain Veteriner. 37(2): 151-159. Wirawan, I.G.K.O., Aoetpah, A., dan Jacob, J.M. 2021. Perbandingan Efektivitas antara Ekstrak Daun Muda Acacia nilotica dengan Desmanthus virgatus terhadap Daya Vermisidal Haemonchus contortus Secara In-vitro. Journal Sain Veteriner. 39(2): 168-177. Yanuartono, Indarjulianto, S., Nururrozi, A., Raharjo, S., dan Purnamaningsih, H. 2020. Penggunaan Antiparasit Ivermectin pada Ternak: Antara Manfaat dan Risiko. Jurnal Sain Peternakan Indonesia. 15(1): 110-123.

DOI: https://doi.org/10.22146/jsv.70779