Korelasi Konsentrasi Testosteron Darah terhadap Kualitas Semen Segar Ayam Kampung Unggul Balitbangtan (KUB) dengan Fenotip Berbeda
Magfira Magfira(1), Ni Wayan Kurniani Karja(2*), R. Iis Arifiantini(3), Tike Sartika(4)
(1) Program Studi Biologi Reproduksi Program Doktoral Sekolah Kedokteran Hewan dan Biomedis Institut Pertanian Bogor
(2) Divisi Reproduksi dan Kebidanan Sekolah Kedokteran Hewan dan Biomedis Institut Pertanian Bogor
(3) Divisi Reproduksi dan Kebidanan Sekolah Kedokteran Hewan dan Biomedis Institut Pertanian Bogor
(4) Pusat Riset Peternakan Organisasi Riset Pertanian dan Pangan Badan Riset dan Inovasi Nasional (BRIN) Bogor, Jawa Barat
(*) Corresponding Author
Abstract
Ayam KUB adalah ayam hasil seleksi dari Balai Penelitian Ternak Ciawi yang memiliki perbedaan fenotip seperti bentuk jengger dan warna bulu. Perbedaan fenotip berpengaruh terhadap konsentrasi testosteron sehingga memengaruhi kualitas semen segar ayam KUB. Penelitian ini bertujuan untuk mengkaji pengaruh fenotip terhadap kualitas semen segar dan testosteron serta korelasinya. Sebanyak 20 ekor ayam KUB umur 1,5 tahun terbagi atas 4 kelompok perlakuan fenotip masing-masing 5 ekor yaitu jengger tunggal bulu penutup merah (JTBM), jenggger tunggal bulu penutup putih (JTBP), jengger pea bulu penutup merah (JPBM), dan jengger pea bulu penutup putih (JPBP). Peubah yang diamati adalah volume, warna, pH dan konsistensi, gerakkan massa, motilitas, viabilitas, abnormalitas dan konsentrasi ejakulat, konsentrasi testosteron dan panjang taji. Hasil penelitian menunjukkan pejantan dengan fenotip JPBM memiliki volume ejakulat paling tinggi dan JTBM adalah yang paling rendah (P<0.05). Pejantan berjengger tunggal memiliki pH lebih tinggi dibandingkan pejantan berjengger pea. Pejantan JTBM juga memiliki motilitas dan konsentrasi ejakulat paling rendah dibandingkan dengan fenotipe lainnya dan untuk peubah warna semen, gerakan massa, viabilitas dan abnormalitas tidak berbeda pada semua fenotip. Panjang taji kiri pejantan JTBP lebih pendek dibandingkan ketiga pejantan lainnya (P<0.05). Ukuran taji berkorelasi positif dengan konsentrasi testosteron dengan kisaran nilai r = 0.33-0.56. Konsentrasi testosteron berkorelasi postif dengan warna (r=0.76), konsentrasi ejakulat (r= 0.44), konsistensi semen (r=0.75) dan motilitas spermatozoa (r= 0.46). Kesimpulan dari penelitian ini adalah pejantan JTBM memiliki kualitas semen segar kurang baik dibandingkan dengan ketiga kelompok pejantan lainnya. Ukuran taji bisa digunakan untuk memprediksi konsentrasi testosteron pada ayam jantan.
Keywords
Full Text:
PDFReferences
Abbas, W., Jabbar, A, Riaz A, Akram M, Ditta YA. (2017). Effect of plumage color and body weight on the semen quality of naked neck chicken. J. World Poult. Res. 7(3): 129-133. Aguilera, E., Amat, J.A. (2007). Carotenoids, immune response and the expression of sexual ornaments in male greenfinches (Carduelis chloris). Naturwissenschaften. 94:895–902 Amao, A.R. (2020). Growth performance traits of meat-type chicken progenies from a broiler line sire and Nigerian indigenous chickens’ dams reared in southern guinea savanna condition of Nigeria. Discovery. 56(289): 66-73 Dasrul, D., Wahyuni, S., Sugito, S., Hamzah, A., Zaini, Z., Haris, A., Gholib, G. (2020). Correlation between testosterone concentrations with scrotal circumference, and semen characteristics in aceh bulls. E3S Web of Conferences. 1st ICVAES 2019 151. Davis, M.F., Ebako, G.M., Morishita T.Y., Mattoon, J.S. (2019). Medical management of the rooster spur. Extension Factsheet, Veterinary Preventive Medicine. The Ohio State Fragoso, J.S., Diaz, M.P., Moreno, J.C.A., Infesta, P.C., Rodiguez-Bertos, A., Barger, K. (2012). Relationships Between Fertility and Some Parameters in Male Broiler Breeders (Body and Testicular Weight, Histology and Immunohistochemistry of Testes, Spermatogenesis and Hormonal Levels). Reprod Dom Anim. 48(2):345-52. Froman, D.P., Pizzari, T., Feltmann, A.J., Castillo-Juarez., Birkhead, T.R. (2001). Sperm mobility: mechanism of fertilizing efficiency, genetic variation and phenotypic relationship with male status in domestic fowl, gallus gallus domesticus. Proc. R. Soc. Lond. B. 269: 607–612 Imsland, F., Feng, C., Boije, H., Bed’hom, B., Fillom, V., Dorshorst, B., Rubin, C.J., Liu, R., Gao, Y., Gu, X., Wang, X., Gourichon, D., Zody, M.C., Zecchin, W., Vieaud, A., Tixier-Boichard, M., Hu, X., Hallbook, F., Li, N., Andersson, L. (2012). The Rose-comb Mutation in Chickens Constitutes a Structural Rearrangement Causing Both Altered Comb Morphology and Defective Sperm Motility. PLoS Genetics. 8(6): 1-13. Jordan, B.K, Shen J.H.-C., Olaso, R., Ingraham H.A., Vllain E. (2003). Wnt4 overexpression disrupts normal testicular vasculature and inhibits testosterone synthesis by repressing steroidogenic factor 1/β-catenin synergy. Proc Natl Acad Sci U S A. 100: (19): 10866-10871. [Kepmen] Keputusan Menteri Pertanian Republik Indonesia Nomor 274/Kpts/SR.120/2/2014 Tentang Pelepasan Galur Ayam KUB-1. 2014 Kirby J.D., Froman D.P. (2000). Reproduction in male birds. In Sturkie's avian physiology, 5th edn (ed. G. Causey Whittow), pp. 597-615. San Diego, CA: Academic Leao, R.A.C., Castro, F.L.S., Xavier, P.R., Vaz D.P., Grazia, J.G.V., Baiao, N.C., Avelar, G.F., Junior, A.P.M. (2016). Comb, cloaca and feet scores and testis morphometry in male broiler breeders at two different ages. Arq. Bras. Med. Vet. Zootec. 69(6). 1529-1538. Lee, Y.J. (2008). Analysis of comb development in chicks with the Pea-comb dominant mutation. Thesis. Uppsala University. Swedia. Lelono, A., Riedstra, B., Groothuis, T.G.G. (2019). The relationship between male social status, ejaculate and circulating testosterone concentration and female tolk androgen transfer in red junglefowl (Gallus gallus). Horm Behav. 116: 104580. McGary, S., Estevez, I., Bakst, M.R., Pollock, D.L. (2002). Phenotypic Traits as Reliable Indicators of Fertility in Male Broiler Breeders. Poult Scie. 81: 102-111. Magfira, M., Arifiantini, R.I., Karja, N.W.K., Darwati, S. (2017). Efektifitas low density lipoprotein dan kuning telur ayam dan puyuh pada pengawetan semen ayam merawang. Jveteriner. 18(3): 345-353. Makhafola, M.B., Umesiobi, D.O., Nedambale, T.L. (2012). Relationship between phenotypic and sperm traits of South African indigenous cockerels. Int. J. Livest. Prod. 3 (6): 61-65 Moghaddam, G., Pourseif, M., Asadpour, R., Rafat, S.A., Jafari-Jozani, R. (2012). Relationship between levels of peripheral blood testosterone, sexual behavior, scrotal circumference and seminal parameters in crossbred rams. Acta Sci. Vet. 40(3): 1049. Navara, K.J., Anderson, E.M., Edwards, M.L. (2012). Comb size and color relate to sperm quality:a test of the phenotype-linked fertility hypothesis. Behav Ecol. doi:10.1093/beheco/ars068 Parker, T.H., Knapp, R., Rosenfield, J.A. (2001). Social mediation of sexually selected ornamentation and steroid hormone levels in male junglefowl. Anim. Behav. 64: 291-298 Peters, A., Astheimer LB., Boland CRJ., Cockburn A. (2000). Testosterone is involved in acquisition and maintenance of sexual selected male plumage in superb fairy-wrens, Malurus cyaneus. Behav. Ecology and Sociobiology. 47: 438-445. Pizzari, T., Jensen, P., Cornwallis, C.K. (2004). A novel of the phenotypic-linked fertility hypothesis reveals independent component of fertility. Proc. R. Soc. Lond. 271: 51-5 Preston, B.T., Stevenson, I.R., Lincoln, G.A., Monfort, S.L., Pilkington, J.G., Wilson, K. (2012). Testes size, testosterone production and reproductive behaviour in a natural mammalian mating system. J. Anim. Ecol. 81: 296-305. Rowe, M., McGraw K.J. (2008). Carotenoids in the seminal fluid of wild bird: intesrspesific variation in the fairy-wrens. Condor. 110: 694-700 Rowe M., Swaddle JP., Pruet-Jones S., Webster MS. (2010). Plumage coloration, ejaculate quality and reproductive phenotype in the red-backed fairy-wren. Anim. Behav. 1-8. Tabatabaei, S., Chaji, M., Mohammadabadi, T. (2010). Correlation between age of rooster and semen quality in Iranian infigenous broiler breeder chickens. J. Anim. Vet. Adv. 9(1): 195-198 Schantz, T.V., Tufvesson, M., Goransson, G., Grahn, M., Wilhelmson, M., Wittzell, H. (1995). Artificial selection for increased comb size and its effects on other sexual characters and viability in Gallus domesticus (the domestic chicken). Heredity. 75: 518-529. Sajjad, M., Ali, S., Ullah, Anwa M., Akhter, S., Andrabi, M.H. (2007). Blood serum testosterone level and its relationship with scrotal circumference and semen characteristics in nili-ravi buffalo bulls. Pakistan Vet. J. 27 (2): 63-66. Sartika, T. (2013). Perbandingan Morfometrik Ukuran Tubuh Ayam KUB-1 dan Sentul Melalui Pendekatan Analisis Diskriminan. In Seminar Nasional Teknologi Peternakan dan Veteriner (hal. 561–570). Sartika, T. (2016). Panen ayam kampung 70 hari. Penebar Swadaya. Jakarta. IDN. Setiadi, D.R., Hasibuan, H., Indriastuti, R., Arif, A.A., Rosyada, Z.N.A., Arifiantini, R.I. (2019). Karakteristik semen ayam IPB-D1. JIPTHP. 7(2): 57-61. Stolt, C.S., Schlierf, A., Lommes, P., Hillgartner, S., Werner, T., Kosian, T., Sock, E., Kessaris, N., Richardson, W.D., Levebvre, V., Wegner, M. (2006). SoxD Proteins Influence Multiple Stages of Oligodendrocyte Development and Modulate SoxE Protein Function Sun, Y., Xue, F., Li, Y., Fu, L., Bai, H.,Ma, H., Xu, S., Chen, J. (2019). Differences in semen quality, testicular histomorphology, fertility, reproductive hormone levels, and expression of candidate genes according to sperm motility in Beijing-You chickens. Poult. Sci. 0: 1-8. Quieroz ,S.A., Cromberg, V.U. (2006). Aggressive behavior in the genus gallus sp. Rev. Bras. Cienc. Avic. 8(1): 01-14. Wragg, D., Mwacharo, J.M., Alcalde, J.A., Hocking, P.M., Hanotte, O. (2012). Analysis of genome-wide structure, diversity and fine mapping of Mendelian traits in traditional and village chickens. Heredity. 109: 6-18.
DOI: https://doi.org/10.22146/jsv.73914
Article Metrics
Abstract views : 705 | views : 1505Refbacks
- There are currently no refbacks.
Copyright (c) 2023 Jurnal Sain Veteriner
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Sain Veteriner Indexed by
Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).
Fakultas Kedokteran Hewan, Universitas Gadjah Mada
Jl. Fauna No.2, Karangmalang, Yogyakarta
Phone: 0274-560862
Fax: 0274-560861
Email: jsv_fkh@ugm.ac.id
HP. 0895363078367
Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
View My Stats