Karagenin sebagai Model Inflamasi pada Kulit Punggung Mencit: Gambaran Makroskopik dan Histopatologis

https://doi.org/10.22146/jsv.86924

Sitarina Widyarini(1*), Sugiyono .(2), Yuli Purwandari Kristianingrum(3), Bambang Sutrisno(4)

(1) Departemen Patologi Fakultas Kedokteran Hewan Universitas Gadjah Mada
(2) Departemen Patologi Fakultas Kedokteran Hewan Universitas Gadjah Mada
(3) Departemen Patologi Fakultas Kedokteran Hewan Universitas Gadjah Mada
(4) Departemen Patologi Fakultas Kedokteran Hewan Universitas Gadjah Mada
(*) Corresponding Author

Abstract


This study aims to create a model of dorsal skin inflammation in mice using carrageenan with various concentrations. Forty-five female Swiss mice with the aged of 8 weeks were used in this study. These mice were divided into three groups of 15. Group I, II and III was given subcutaneous injection of 1%, 2% and 4% carrageenan respectively on the dorsum. Carrageenan was dissolved in 0.9% buffer saline with 0.1 mL volume injection. Prior to carrageenan injection, dorsal skin-fold thickness was measured using calipers and then was repeated every hour up to 6 hours. At the end of the sixth hour of measuring skin-fold thickness, animal was sacrificed, the dorsal skin was excised and then was fixed in 10% buffer formalin for histopathological examination. Data from skin-fold thickness measurement was analyzed using Two Ways Anova followed by Tukey HSD test. Results of this study demonstrate that there was a significant difference in skin-fold thickness between treatment groups (p<0.05). Sub cutaneous injection of 1%, 2% and 4% carrageenan resulted in increasing skin-fold thickness approximately 35%, 50% and 61% at the one-hour post carrageenan injection respectively (p<0.05). Skin-fold thickness gradually decreased at the second to the sixth hour after carrageenan injection. Histopathological examination shows mild and moderate dermal inflammation in the group given 1% and 2% carrageenan respectively. Severe dermal and panniculus inflammation was found in the group given 4% carrageenan. To conclude, carrageenan could be used to induce inflammation in back skin of mice.

Keywords


carrageenan; inflammation skin-fold thickness; skin

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DOI: https://doi.org/10.22146/jsv.86924

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