β-Sitosterol: The Isolated Compound from n-Hexane Fraction of Baccaurea racemosa (Reinw. ex Blume) Müll. Arg. Pulp and Its Antioxidant Activity

  • Lina Permatasari Department Of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
  • Sugeng Riyanto Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
  • Abdul Rohman Center of Excellence, Institute for Halal Industry and System, Universitas Gadjah Mada, Yogyakarta, Indonesia
DOI: https://doi.org/10.22146/ijcpa.3693
Keywords: n-Hexane fraction; Antioxidant activity; Baccaurea racemosa pulp; β-sitosterol; Isolation

Abstract

Baccaurea racemosa is one of the most widespread plants in Indonesia. The constituent compound at Baccaurea racemosa influenced its biological activity. However, the isolation of compounds from the n-hexane fraction of B. racemosa pulp has never been reported. This study aimed to isolate and spectroscopic analysis of compound from n-hexane fraction of Baccaurea racemosa pulp and its antioxidant activity. The fraction of n-hexane was isolated using the preparative thin-layer chromatography (PLC) method. The purified compound was identified its structure compound using the spectroscopic method. Then the antioxidant activity was determined using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. UV-Vis spectrum of isolated compounds revealed the absence of conjugated double bonds. Furthermore, the infrared spectrum showed OH, C-H aliphatic, C-O, C-H vanillic, and C=C groups. The molecular ion of the isolated compound was 414 g/mol. The NMR spectrum showed several peaks at various chemical shifts. Based on the spectral analysis, the isolated compound was indicated as β-sitosterol. This compound has antioxidant activity with IC50 > 2.4155 mM.

References

1. Maskur. 10 April 2014. Kepundung/Menteng (Baccaurea racemosa (Reinw.)), (Online), (https://forestryinformation.wordpress.com, accessed 8 18 Agustus 2018)
2. Mann, S.; Sharma, A.; Biswas, S.; Gupta RK. Identification and molecular docking analysis ofactive ingredients with medicinal properties from edible Baccaurea sapida. Bioinformation 2015, 11, 437–443.
3. Mokhtar, S.I.; Leong, P.C.; Ven, L.E.; Aziz, N.A.A. Total phenolic contents, antioxidant activities and organic acids composition of three selected fruit extracts at different maturity stages. J. Trop. Resour. Sci 2014, 2, 40–46.
4. Erwin, E.; Pusparohmana, W.R.; Sari, I.P.; Hairani, R.; Usman, U. Phytochemical and antioxidant activity evaluation of the bark of Tampoi (Baccaurea macrocarpa). F1000research 2018, 7, 1–9.
5. Mann, S.; Satpathy, G.; Gupta, R.K. Evaluation of nutritional and phytochemical profiling of Baccaurea ramiflora Lour. syn. Baccaurea sapida (Roxb.) Mull. Arg. fruits. Indian j. tradit. knowl 2016, 15, 135–142.
6. Pan, Z-H.; Ning, D-S.; Huang, S-S.; Wu, Y-F.; Ding, T.; Luo, L. A new picrotoxane sesquiterpene from the berries of Baccaurea ramiflora with antifungal activity against Colletotrichum gloeosporioides. Nat. Prod. Res. 2015, 29, 1323–1327.
7. Yang, X-W.; He, H-P.; Ma, Y-L.; Wang, F.; Zuo, Y-Q.; Lin, H.; Li, S-L.; Li, L.; Hao, X-J. Three new vanilloid derivatives from the stems of Baccaurea ramiflora. Planta Med 2010, 76, 88–90.
8. DeSheng, N.; YunFei, W.; Lü; ShiHong; ZhengHong, P. Chemical constituents of the stem and leaves of Baccaurea ramiflora. Guihaia 2014, 34, 160–166.
9. Xu, J.; Guan, H-S.; Lin, Q. A new sesquiterpene lactone from root of Baccaurea ramiflora. Chin. Tradit. Herb. Drugs 2007, 38, 1450–1462.
10. Mandal, M.; Kabir, L.; Khan, I.; Haque, S.T.; Haque, Md.E. Isolation of bioactive principles and studies of antimicrobial, cytotoxic and antioxidant activities of the stem bark of Baccaurea ramiflora (Euphorbiaceae). Bioresearch Communications-(BRC) 2018, 4, 565–571.
11. Ghaneian, M.T.; Ehrampoush, M.H.; Jebali, A.; Hekmatimoghaddam, S.; Mahmoudi, M. Antimicrobial activity, toxicity and stability of phytol as a novel surface disinfectant. Environ. Health Eng. Manag. J. 2015, 2, 13–16.
12. Pejin, B.; Ciric, A.; Glamoclija, J.; Nikolic, M.; Sokovic, M. In vitro anti-quorum sensing activity of phytol. Nat. Prod. Res. 2015, 29, 374–377.
13. Yang, X-W.; Wang, J-S.; Ma, Y-L.; Xiao, H-T.; Zuo, Y-Q.; Lin, H.; He, H-P.; Li, L.; Hao, X-J. Bioactive phenols from the leaves of Baccaurea ramiflora. Planta Medica 2007, 73, 1415–1417.
14. Permatasari, L.; Riyanto, S.; Rohman, A. Identification of phenolic compound from active subfraction of Baccaurea racemosa Pulp by high resolution mass spectrometry. Int. J. Pharm. Res. 2020, 13, 1065–1072.
15. Widodo, H.; Sismindari, S.; Asmara, W.; Rohman, A. Antioxidant activity, total phenolic and flavonoid contents of selected medicinal plants used for liver diseases and its classification with chemometrics. J App Pharm Sci 2019, 9, 99–105.
16. Wulandari, L.; Nugraha, A.S.; Azhari, N.P. Penentuan aktivitas antioksidan dan antidiabetes ekstrak daun kepundung (Baccaurea racemosa Muell.Arg.) secara In Vitro. J Sains Farm Klin 2020, 7, 60–66.
17. Elsa, C., 2020, Uji aktivitas antibakteri dan analisis gc-ms ekstrak daun kepundung (Baccaurea racemosa (reinw. ex blume) mull. arg) terhadap Escherichia coli dan Staphylococcus aureus. Thesis, University of Mataram, Indonesia.
18. Permatasari, L.; Riyanto, S.; Rohman, A. Isolation and spectroscopic analysis of compounds from Baccaurea racemosa (Reinw. ex Blume) Müll. Arg. Pulp. Trop. J. Nat. Prod. Res. 2021, 5, 1434–1438.
19. Moreno, J.J. Effect of olive oil minor components on oxidative stress and arachidonic acid mobilization and metabolism by macrophages RAW 264.7. Free Radic Biol Med 2003, 35, 1073–1081.
20. Baskar, A.A.; Numair, K.S.A.; Paulraj, M.G.; Alsaif, M.A.; Muamar, M.A.; Ignacimuthu S. β-sitosterol prevents lipid peroxidation and improves antioxidant status and histoarchitecture in rats with 1,2-dimethylhydrazine-induced colon cancer. J Med Food 2012, 15, 335–343.
Published
2021-12-29
How to Cite
Permatasari, L., Riyanto, S., & Rohman, A. (2021). β-Sitosterol: The Isolated Compound from n-Hexane Fraction of Baccaurea racemosa (Reinw. ex Blume) Müll. Arg. Pulp and Its Antioxidant Activity. Indonesian Journal of Chemometrics and Pharmaceutical Analysis, 1(3), 156-163. https://doi.org/10.22146/ijcpa.3693
Issue
Vol 1 No 3 (2021)
Section
Original Articles

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