Effect of Methyl Jasmonic Acid and Tryptophan on Increasing Vincristine Content of Leaf Cell Suspension Culture Catharanthus roseus (L.) G. Don.
Abstract
Catharanthus roseus (L.) G. Don known as tapak dara is a medicinal plant that produces alkaloid compounds such as vincristine as an anticancer which has very high economic value and is produced in very small concentrations. This study aims to increase the levels of vincristine in cell suspension cultures of tapak dara leaves by administering methyl jasmonic acid and tryptophan with various concentrations. Tapak dara leaf explants were grown on solid Murashige-Skoog (MS) media supplemented with the addition of 2.4 D 1 mg/L growth regulator and 4 mg/L kinetin. The callus was subcultured and then suspension culture was carried out on liquid MS medium by administering methyl jasmonic acid and tryptophan with various concentrations. Callus growth was carried out by weighing the fresh weight and dry weight of the callus. The vincristine content and assay were analyzed using High Performance Liquid Chromatography (HPLC). Callus growth in cell suspension cultures with X50Y300 treatment resulted in the highest fresh weight of 1.094 g, while the highest dry weight was found in treatment X75Y200 of 0.166 g. The results of the HPLC analysis showed that the administration of methyl jasmonic acid and tryptophan with various concentrations on tapak dara leaf cell suspension cultures were able to produce vincristine compounds. In treatment, X50Y100, X75Y100, X100Y100, X50Y200, X75Y200, and X75Y300 were able to increase vincristine levels, but the X100Y200, X50Y300, and X100Y300 treatments decreased vincristine levels. The highest levels of vincristine were found in treatment X50Y200 (50 mg/L tryptophan and 200 μM methyl jasmonic acid ) which was 49.311 ppm .
References
Aslam, J., Siddiqui, Z. H., Zohra, F., Khan, S. H., Fatima, Z., Maqsood, M., Bhat, A., Nasim, S. A., Ilah, A., Ahmad, I. Z., Khan, S. A., Mujib, A., & Sharma, M. P. (2010). Catharanthus roseus (L.) G. Don. An Important Drug: It’s Applications And Production. Pharmacie Globale (IJCP), 01(4), 1–16. https://www.researchgate.net/publication/49596279
Baenas, N., García-Viguera, C., & Moreno, D. A. (2014). Elicitation: A tool for enriching the bioactive composition of foods. In Molecules (Vol. 19, Issue 9, pp. 13541–13563). MDPI AG. https://doi.org/10.3390/molecules190913541
Darsini, N. N. (2011). Perkembangan Latisifer Pada Kultur Kalus Catharanthus roseus (L) G. Don Yang Diinduksi Dengan Kombinasi Zat Pengatur Tumbuh Kinetin + NAA. Jurnal Biologi, 15(2), 34–38.
Endt, D. Vom, Kijne, J. W., & Memelink, J. (2002). Molecules of Interest Transcription factors controlling plant secondary metabolism: what regulates the regulators? Phytochemistry, 61, 107–114.
Gardner, F. P., R.B. Pearce, & R.L. Mitchel. (1991). Fisiologi Tanaman Budidaya . Penerjemah: H. Susilo. Jakarta: UI Press.
Ibrahim, M., Danial, N., Matter, M., & Rady, M. (2021). Effect of light and methyl jasmonate on the accumulation of anticancer compounds in cell suspension cultures of Catharanthus roseus. Egyptian Pharmaceutical Journal, 20(4), 294–302. https://doi.org/10.4103/epj.epj_48_21
Laksmi K, G. dan Santosa, D., 2021. Pengaruh Penambahan Triptofan Terhadap Peningkatan Kadar Senyawa Vinkristin dan Vinblastin Pada Kalus Daun Tapak Dara (Catharanthus roseus (L.) G. Don.) : Hasil Penelitian dan Narrative Review. Skripsi. Universitas Gadjah Mada.
Lombonbitung, E., W. Tilaar, W., & Pandiangan, D. (2015). Kandungan Vinkristin Pada Kultur Kalus Catharaanthus roseus (L) G. Don Yang Diberi Perlakuan Triptofan Dan Vindolin. Jurnal Ilmiah Farmasi, 4(4), 127–138.
Muryanti, Solichatun, & Anggarwulan, E. (2005). Pertumbuhan dan Produksi Reserpin Kalus Pule Pandak (Rauvolfia serpentina) pada Pemberian Metil Jasmonat secara in Vitro. Bioteknologi, 2(2), 58–64. https://doi.org/10.13057/biotek/c020205
Namdeo, A. G. (2007). Plant Cell Elicitation for Production of Secondary Metabolites: A Review. Pharmacognosy Reviews, 1(1), 69–79. http://www.phcogrev.com
Ncube, B., & Van Staden, J. (2015). Tilting plant metabolism for improved metabolite biosynthesis and enhanced human benefit. In Molecules (Vol. 20, Issue 7, pp. 12698–12731). MDPI AG. https://doi.org/10.3390/molecules200712698
Pandiangan, D., & Nainggolan, N. (2006). Produksi Alkaloid dari Kalus Tapak Darah. Jurnal Ilmiah Sains, 6, 48–54.
Rady, M. R. (2019). Plant Biotechnology and Medicinal Plants. In Plant Biotechnology and Medicinal Plants. Springer International Publishing. https://doi.org/10.1007/978-3-030-22929-0
Ratnadewi, D., Satriawan, D., & Sumaryono. (2013). Enhanced Production Level Of Quinine In Cell Suspension Culture Of Cinchona ledgeriana moens By Paclobutrazol. Biotropia, 20(1), 10–18. https://doi.org/10.11598/btb.2013.20.1.10
Rijhwani, S. K., & Shanks, J. V. (1998). Effect of Elicitor Dosage and Exposure Time on Biosynthesis of Indole Alkaloids by Catharanthus roseus Hairy Root Cultures. Biotechnol. Prog, 14(3), 442–449.
Sartika, D., & Santosa, D. (2012). Pengaruh Kombinasi Zat Pengatur Tumbuh (2,4 D Dan Kinetin) Terhadap Pertumbuhan Dan Kandungan Metabolit Sekunder Pada Kalus Phaleria macrocarpa (Scheff.) Boerl. ). 5(1), 53–62.
Shanks, J. V, Bhadra, R., Morgan, J., Rijhwani, S., & Vani, S. (1998). Quantification of Metabolites in the Indole Alkaloid Pathways of Catharanthus roseus: Implications for Metabolic Engineering. In Bio-technol Bioeng (Vol. 58).
Shukla, A. K., Shasany, A. K., Gupta, M. M., & Khanuja, S. P. S. (2006). Transcriptome analysis in Catharanthus roseus leaves and roots for comparative terpenoid indole alkaloid profiles. Journal of Experimental Botany, 57(14), 3921–3932. https://doi.org/10.1093/jxb/erl146
Wagih, M. E., Alam, G., Wiryowidagdo, S., & Attia, K. (2008). Improved production of the indole alkaloid canthin-6-one from cell suspension culture of Brucea javanica (L.) Merr. Indian Journal of Science and Technology, 1(7), 1–6. https://doi.org/10.17485/ijst/2008/v1i7/29591
Wasternack, C. (2007). Jasmonates: An update on biosynthesis, signal transduction, and action in plant stress response, growth, and development. In Annals of Botany (Vol. 100, Issue 4, pp. 681–697). https://doi.org/10.1093/aob/mcm079
Xing, S.-H., & Jiao, S. (2011). Effect of plant growth regulator combinations on the biosynthesis of terpenoid indole alkaloids in Catharanthus roseus Trichome development View project metabolic engineer View project. In Article in Journal of Medicinal Plant Research. https://www.researchgate.net/publication/228836102
Zhao, J., Hu, Q., Guo, Y. Q., & Zhu, W. H. (2001). Effects of stress factors, bioregulators, and synthetic precursors on indole alkaloid production in compact callus clusters cultures of Catharanthus roseus. Applied Microbiology and Biotechnology, 55(6), 693–698. https://doi.org/10.1007/s002530000568
Zhou, M.-L., Hou, H.-L., Zhu, X.-M., Shao, J.-R., Wu, Y.-M., & Tang, Y.-X. (2010). Molecular regulation of terpenoid indole alkaloids pathway in the medicinal plant, Catharanthus roseus. Journal of Medicinal Plants Research, 4(25), 2760–2772. http://www.academicjournals.org/JMPR
