Hair Serum Nanoemulsion loaded Clove Essential Oil Formulation for Androgenetic Alopecia: Characterization and Hair Growth Activity
Abstract
Androgenetic alopecia (AA) is hair loss caused by genetic factor and androgen hormone. Clove essential oil (CEO) contain eugenol which has anti-androgen activity and can be used to treat AA. Formulation CEO into hair serum nanoemulsion overcome the CEO from several disadvantages. The aims of this study are preparation, characterization, and evaluation hair growth activity of hair serum nanoemulsion loaded CEO. The chemical components of CEO were analyzed by gas chromatography. The optimum formula was obtained using the D-Optimal Design method and was further characterized for droplet size, size distribution, pH, viscosity, thermodynamic stability, and storage stability. In vivo of skin irritation and hair growth activity studies were evaluated. The result showed the GC-MS analysis of the CEO consisted of eight components, which the main component is eugenol (60.72%). The optimum formula consists CEO (10.63%), cremophor RH 40 (67.5%), and propylene glycol (21.87%). Formula has the droplet size of 31.26±0.155 nm, the PDI value of 0.24±0.008, the pH of 5.55±0.01, the viscosity of 2386.18±62.77 cP, thermodynamically and physically stable, and doesn’t showed the skin irritation. The length and weight hair of the treatment group was 2.28±0,12 cm and 2.28±0,12 g, respectively. The hair serum nanoemulsion loaded CEO was showed the highest of hair growth activity, demonstrating the preparation has effect to promote the hair growth and potential for promising hair growth nutraceutical to AA treatment.
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