Hair Serum Nanoemulsion loaded Clove Essential Oil Formulation for Androgenetic Alopecia: Characterization and Hair Growth Activity

Wayan Cintya Ganes  Budastra; Teti Mariam  Riandari; Laras  Novitasari; Retno Murwanti; Ronny Martien

doi:10.22146/ijp.12159

Wayan Cintya Ganes Budastra Department of Pharmacy, Faculty of Medicine and Health Sciences, University of Mataram, Mataram, 83115, Indonesia; Master of Pharmacy Science, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara 55281, Yogyakarta, Indonesia
Teti Mariam Riandari Master of Pharmacy Science, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara 55281, Yogyakarta, Indonesia
Laras Novitasari Master of Pharmacy Science, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara 55281, Yogyakarta, Indonesia; Researcher, PT. Ronature Teknologi Indonesia, Yogyakarta, 55188, Indonesia.
Retno Murwanti Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara 55281, Yogyakarta, Indonesia
Ronny Martien Department of Pharmaceutics, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara 55281, Yogyakarta, Indonesia

Keywords: Clove oil, Hair serum, Nanoemulsion, Hair growth

Abstract

Androgenetic alopecia (AA) is hair loss caused by genetic factor and androgen hormone. Clove essential oil (CEO) contain eugenol which has anti-androgen activity and can be used to treat AA. Formulation CEO into hair serum nanoemulsion overcome the CEO from several disadvantages. The aims of this study were preparation, characterization, and evaluation hair growth activity of hair serum nanoemulsion loaded CEO. The chemical components of CEO were analysed by gas chromatography. The optimum formula was obtained using the D-Optimal Design method and was further characterized for droplet size, size distribution, pH, viscosity, thermodynamic stability, and storage stability. In vivo of skin irritation and hair growth activity were evaluated. The result showed that the GC-MS analysis of the CEO consisted of eight components, which the main component was eugenol (60.72%). The optimum formula consisted of CEO (10.63%), cremophor RH 40 (67.5%), and propylene glycol (21.87%). Formula has the droplet size of 31.26±0.155 nm, the PDI value of 0.24±0.008, the pH of 5.55±0.01, the viscosity of 2386.18±62.77 cP, physically stable, and did not show the skin irritation. The length and weight hair of the treatment group was 2.28±0.12 cm and 2.28±0.12 g, respectively. Clove oil nanoemulsion hair serum is effective in promoting hair growth and may serve as a promising treatment product for alopecia areata.

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