Secondary Metabolite Screening of Marine Bacteria Roseivirga sp. PAP.19 and Sinomicrobium sp. PAP.21 as an Antimicrobial using the One Strain Many Compounds (OSMAC) Approach

Anita Dilla  Harfiyani; Riyanti; Maria Dyah Nur  Meinita; Riviani Riviani; Harwoko  Harwoko; Ute  Mettal

doi:10.22146/jtbb.22572

Anita Dilla Harfiyani Faculty of Fisheries and Marine Science, Jenderal Soedirman University, Purwokerto 53123, Central Java, Indonesia
Riyanti Faculty of Fisheries and Marine Science, Jenderal Soedirman University, Purwokerto 53123, Central Java, Indonesia https://orcid.org/0000-0003-0665-8092
Maria Dyah Nur Meinita Faculty of Fisheries and Marine Science, Jenderal Soedirman University, Purwokerto 53123, Central Java, Indonesia https://orcid.org/0000-0002-6334-4172
Riviani Riviani Faculty of Fisheries and Marine Science, Jenderal Soedirman University, Purwokerto 53123, Central Java, Indonesia https://orcid.org/0009-0008-7803-9075
Harwoko Harwoko Department of Pharmacy, Faculty of Health Sciences, Jenderal Soedirman University, Purwokerto 53123, Central Java, Indonesia https://orcid.org/0000-0002-4111-7147
Ute Mettal Institute for Insect Biotechnology, Justus Liebig University Giessen, 35392 Giessen, Germany https://orcid.org/0009-0005-5652-2275

DOI: https://doi.org/10.22146/jtbb.22572

Keywords: Antimicrobials, Co-culture, Roseivirga sp. PAP.19, Sinomicrobium sp. PAP.21, OSMAC Approach

Abstract

One of the most promising sources for the discovery and development of new antibiotics is marine microorganisms from the phylum Bacteroidetes. However, the output of exploration campaigns exploiting the Bacteroidetes phylum still needs to be improved. Co-culture represents an approach for discovering new metabolites that increases metabolite diversity and avoids the rediscovery of known metabolites. This research aims to determine the antimicrobial potential of the extracts derived from the co-culture of the bacteria Roseivirga sp. PAP.19 and Sinomicrobium sp. PAP.21. In this study, Roseivirga sp. PAP.19 and Sinomicrobium sp. PAP.21 were co-cultured under different culture conditions, following the principle of the One Strain Many Compounds (OSMAC) approach, to enhance metabolite diversity. The resulting extracts were analysed in a time-based fashion by molecular networking of the LC-HRMS profile. The OSMAC principle was applied to activate the production of secondary metabolite biosynthetic gene clusters (BGCs) and thereby hopefully trigger the production of new antimicrobial compounds. Extracts of the co-culture produced various secondary metabolites. In our study, we detected surfactin C13 (m/z 1008.65 [M+H]), surfactin C14 (m/z 1022.67 [M+H]), and surfactin C15 (m/z 1036.69 [M+H]⁺). These metabolites are known to possess antimicrobial activity with potential applications against pathogenic bacteria.

References

Abbas, S. & Mahmoud, H., 2022. Identification of Sponge-Associated Bacteria from the Coast of Kuwait and Their Potential Biotechnological Applications. Frontiers in Microbiology, 13, 896718. doi: 10.3389/fmicb.2022.896718.
Ali, N. et al., 2022. Lipopeptide Biosurfactants from Bacillus spp.: Types, Production, Biological Activities, and Applications in Food. Journal of Food Quality. doi: 10.1155/2022/3930112.
Caudal, F., Tapissier-Bontemps, N. & Edrada-Ebel, R.A., 2022. Impact of Co-Culture on the Metabolism of Marine Microorganisms. Marine Drugs, 20(2), 153. doi: 10.3390/md20020153.
Centers for Disease Control and Prevention., 2019. Antibiotic Resistance Threats in the United States, 2019. Atlanta, Georgia. doi: 10.15620/cdc:82532.
Gavriilidou, A. et al., 2020. Comparative genomic analysis of Flavobacteriaceae: Insights into carbohydrate metabolism, gliding motility and secondary metabolite biosynthesis. BMC Genomics, 21(1). doi: 10.1186/s12864-020-06971-7.
Gomes, N.G.M. et al., 2016. Marine invertebrate metabolites with anticancer activities: Solutions to the “supply problem”. Marine Drugs, 14(5), 98. doi: 10.3390/md14050098.
Jemil, N. et al., 2017. Antioxidant properties, antimicrobial and anti-adhesive activities of DCS1 lipopeptides from Bacillus methylotrophicus DCS1. BMC Microbiology, 17(1), 144. doi: 10.1186/s12866-017-1050-2.
Jung, Y.T. et al., 2016. Roseivirga maritima sp. nov., isolated from seawater. International Journal of Systematic and Evolutionary Microbiology, 66(7), pp.2664–2670. doi: 10.1099/ijsem.0.001102.
Li, X. et al., 2023. Exploring Diverse Bioactive Secondary Metabolites from Marine Microorganisms Using Co-Culture Strategy. Molecules, 28(17), 6371. doi: 10.3390/molecules28176371.
Lipphardt, A. et al., 2023. Identification and quantification of biosurfactants produced by the marine bacterium Alcanivorax borkumensis by hyphenated techniques. Analytical and Bioanalytical Chemistry, 415(29–30), pp.7067–7084. doi: 10.1007/s00216-023-04972-5.
Mustapha, Z., Mat, N., Othman, R. & Zakaria, A.J., 2017. Growth of BRIS Soil Bacteria in Organic Material and Potassium Nitrate. Proceedings of the 5th International Conference on Chemical, Agricultural, Biological and Environmental Sciences (CAFES-17), Kyoto, Japan, 18–19 April 2017. doi: 10.15242/DIRPUB.DIR0417205.
Navarro Llorens, J.M., Tormo, A. & Martínez-García, E., 2010. Stationary phase in gram-negative bacteria. FEMS Microbiology Reviews, 34(4), pp.476–495. doi: 10.1111/j.1574-6976.2010.00213.
Özkaya, F.C. et al., 2018. Induction of new metabolites from sponge-associated fungus Aspergillus carneus by OSMAC approach. Fitoterapia, 131, pp.9–14. doi: 10.1016/j.fitote.2018.10.008.
Pan, R. et al., 2019. Exploring structural diversity of microbe secondary metabolites using OSMAC strategy: A literature review. Frontiers in Microbiology, 10, 294. doi: 10.3389/fmicb.2019.00294.
Riyanti et al., 2020. Selection of sponge-associated bacteria with high potential for the production of antibacterial compounds. Scientific Reports, 10(1), 18985. doi: 10.1038/s41598-020-76256-2.
Riyanti et al., 2023. Draft Genome Sequences of Algoriphagus sp. Strain PAP.12 and Roseivirga sp. Strain PAP.19, Isolated from Marine Samples from Papua, Indonesia. Microbiology Resource Announcements, 12(4). doi: 10.1128/mra.01264-22.
Srinivasan, R. et al., 2021. Marine bacterial secondary metabolites: A treasure house for structurally unique and effective antimicrobial compounds. Marine Drugs, 19(10), 530. doi: 10.3390/md19100530.
Thawabteh, A.M. et al., 2023. Antifungal and Antibacterial Activities of Isolated Marine Compounds. Toxins, 15(2), 93. doi: 10.3390/toxins15020093.
Vitale, G.A. et al., 2020. Molecular network and culture media variation reveal a complex metabolic profile in Pantoea cf. eucrina D2 associated with an acidified marine sponge. International Journal of Molecular Sciences, 21(17), 6307. doi: 10.3390/ijms21176307.
Xu, B.H. et al., 2018. Isolation and characterization of cyclic lipopeptides with broad-spectrum antimicrobial activity from Bacillus siamensis JFL15. 3 Biotech, 8(10), 444. doi: 10.1007/s13205-018-1443-4.