Antibacterial Potential of Kecombrang (Etlingera elatior) Leaf Extract as a Natural Ingredient in Facial Cleansing Soap
Sri Purwati(1), Jailani Jailani(2), Muhammad Ridho(3), Volta Kelik Setiawan(4), Rimbawan Apriadi(5*)
(1) Department of Biology Education, Faculty of Teacher Training and Education, Mulawarman University, Indonesia
(2) Department of Biology Education, Faculty of Teacher Training and Education, Mulawarman University, Indonesia
(3) Department of Biology Education, Faculty of Teacher Training and Education, Mulawarman University, Indonesia
(4) Department of Biology Education, Faculty of Teacher Training and Education, Mulawarman University, Indonesia
(5) Department of Agricultural Products Technology, Faculty of Agriculture, Mulawarman University, Samarinda, East Kalimantan 75119, Indonesia
(*) Corresponding Author
Abstract
Kecombrang (Etlingera elatior) leaves are known to contain bioactive compounds such as tannins, flavonoids, and saponins with antibacterial properties. This study aimed to evaluate the antibacterial potential of kecombrang leaf extract and its application in facial cleansing soap formulation against Staphylococcus aureus. The extract was prepared by ethanol maceration, phytochemical screening was conducted, and antibacterial activity was tested using the well diffusion method. The extract showed the highest antibacterial activity at a 40% concentration, producing an inhibition zone of 10.4 ± 0.34 mm. Furthermore, the extract was incorporated into liquid soap formulations with varying potassium hydroxide (KOH) amounts (50–84 g) and reaction times (35–65 min). The optimized formula with 67 g KOH and 65 min reaction time exhibited the largest inhibition zone of 13.28 ± 0.41 mm, while the formula with 50 g KOH and 35 min reaction time showed the lowest free fatty acid (FFA) level (1.541%), making it safer for daily use. These findings indicate that kecombrang leaf extract has promising potential as a natural antibacterial ingredient in environmentally friendly facial cleansing soap.
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Abba, D., Inabo, H. I., Yakubu, S. E., & Olonitola, O. S., 2009. Antibacterial activity of some powdered herbal preparations marketed in Kaduna metropolis. Sci World J 4(1). https://doi.org/10.4314/swj.v4i1.51833
Alnuqaydan, A. M., 2024. The dark side of beauty: an in-depth analysis of the health hazards and toxicological impact of synthetic cosmetics and personal care products. Frontiers in public health 12, 1439027. https://doi.org/10.3389/fpubh.2024.1439027
Anzian, A., Muhialdin, B., Mohammed, N., Kadum, H., Marzlan, A., Sukor, R., & Hussin, A., 2020. Antibacterial Activity and Metabolomics Profiling of Torch Ginger (Etlingera elatior Jack) Flower Oil Extracted Using Subcritical Carbon Dioxide (CO2). Evidence-Based Complementary and Alternative Medicine 2020(1), 4373401. https://doi.org/10.1155/2020/4373401
Bernatoniene, R., Bernatoniene, J., & Ramanauskiene, K., 2004. The analysis of tincture for improvement of blood circulation. Medicina (Kaunas, Lithuania) 40(8), 758-761.
Binugraheni, R., & Larasati, N. T., 2020. Antibacterial Activity Test of Leaves Kecombrang (Nicolaia Speciosa) Ethanolic Extracts Against Staphylococcus Aureus. Journal of Health (JoH) 7 (2), 51-58.
Book: Badan Standardisasi Nasional., 2016. Standar Mutu Sabun Mandi. [Bath Soap Quality]. Standards SNI 3532-2016. Dewan Standarisasi Nasional. Jakarta.
Book: Fessenden, R. J., & Fessenden, J. S., 1982. Kimia organik jilid 2. [Organic chemistry volume 2]. Erlangga.
Book: Standar Nasional Indonesia. 1996. Sabun mandi cair. [Liquid bath soap]. Badan Standarisasi Nasional.
Desbois, A., & Lawlor, K., 2013. Antibacterial Activity of Long-Chain Polyunsaturated Fatty Acids against Propionibacterium acnes and Staphylococcus aureus. Marine Drugs 11(11), 4544 - 4557. https://doi.org/10.3390/md11114544
Detha, A., Datta, F. U., Beribe, E., Foeh, N., & Ndaong, N., 2018. Efektivitas bakteri asam laktat yang disolasi dari susu kuda sumba terhadap kualitas silase jerami padi. [The effectiveness of lactic acid bacteria isolated from Sumba horse milk on the quality of rice straw silage]. J Kaji Vet 6(1), 31-37. https://doi.org/10.35508/jkv.v6i1.1053
Guo, Y., Song, G., Sun, M., Wang, J., & Wang, Y., 2020. Prevalence and Therapies of Antibiotic-Resistance in Staphylococcus aureus. Frontiers in Cellular and Infection Microbiology 10, 107. https://doi.org/10.3389/fcimb.2020.00107
Hasibuan, R., Adventi, F., & Rtg, R., 2019. Pengaruh Suhu Reaksi, Kecepatan Pengadukan Dan Waktu Reaksi Pada Pembuatan Sabun Padat Dari Minyak Kelapa (Cocos nucifera L.). [The Effect of Reaction Temperature, Stirring Speed and Reaction Time on Making Solid Soap from Coconut Oil (Cocos nucifera L.)]. Jurnal Teknik Kimia USU 8(10), 11-17.
Lahare, R. P., Yadav, H. S., Bisen, Y. K., & Dashahre, A. K., 2021. Estimation of total phenol, flavonoid, tannin and alkaloid content in different extracts of Catharanthus roseus from Durg district, Chhattisgarh, India. Sch. Bull 7(1), 1-6. https://doi.org/10.36348/sb.2021.v07i01.001
Lee, A., Lencastre, H., Garau, J., Kluytmans, J., Malhotra-Kumar, S., Peschel, A., & Harbarth, S., 2018. Methicillin-resistant Staphylococcus aureus. Nature Reviews Disease Primers 4(1), 1-23. https://doi.org/10.1038/nrdp.2018.33
Lo, C., Lai, Y., Liu, Y., Gallo, R., & Huang, C., 2011. Staphylococcus aureus hijacks a skin commensal to intensify its virulence: immunization targeting β-hemolysin and CAMP factor. The Journal of investigative dermatology 131(2), 401-409. https://doi.org/10.1038/jid.2010.319
Marzlan, A., Muhialdin, B., Abedin, N., Mohammed, N., Abadl, M., Roby, B., & Hussin, A., 2020. Optimized supercritical CO2 extraction conditions on yield and quality of torch ginger (Etlingera elatior (Jack) R.M. Smith) inflorescence essential oil. Industrial Crops and Products 154, 112581. https://doi.org/10.1016/j.indcrop.2020.112581
Maser, W., Purwoko, A., Yuliana, N., Lubis, L., & Khatib, A. 2022. GC-MS Based Metabolite Profiling and Antibacterial Activity of Torch Ginger (Etlingera elatior) Flowers Extract. Indonesian Journal of Chemistry 22(4), 1014-1024.
Morales, G., Sierra, P., Mancilla, A., Paredes, A., Loyola, L. A., Gallardo, O., & Borquez, J., 2003. Secondary metabolites from four medicinal plants from northern Chile: Antimicrobial activity and biotoxicity against Artemia salina. Bol la Soc Chil Quim 48(2), 13-18. https://doi.org/10.4067/S0717-97072003000200002
Mu’nisa, A., Wresdiyati, T., Kusumorini, N., & Manalu. W., 2012. Aktivitas Antioksidan Ekstrak Daun Cengkeh. [Antioxidant Activity of Clove Leaf Extract]. Jurnal Veteriner 13(3), 272-277.
Poelongan, M., Chairul, Komala, I., Salmah, S., & Susan, M. N., 2006. Aktivitas Antimikroba dan Fitokimia dari Beberapa Tanaman Obat. [Antimicrobial and Phytochemical Activities of Some Medicinal Plants]. Seminar Nasional Teknologi Peternakan dan Veteriner.
Pulungan, A., Sitepu, D., & Sinaga, D., 2018. Formulation Of Ointment Of Antibactery Ethanol Extract Of Torch Ginger (Etlingera elatior) Against Bacteria Staphylococcus aureus. Jusnirnal Penelitian Farmasi & Herbal 1(1), 1-5. https://doi.org/10.36656/jpfh.v1i1.30
Samosir, S. R., Siagian, H., & Purba, S. 2024. Uji Efektivitas Antibakteri Kombinasi Ekstrak Etil Asetat Daun Rimbang (Solanum torvum Sw.) Dan Daun Kecombrang (Etlingera elatior (Jack) RM sm.) Terhadap Staphylococcus Aureus dan Escherichia Coli. [Antibacterial Effectiveness Test of Combination of Ethyl Acetate Extract of Rimbang Leaves (Solanum torvum Sw.) and Torch Ginger Leaves (Etlingera elatior (Jack) RM sm.) Against Staphylococcus Aureus and Escherichia Coli.] JIFI (Jurnal Ilmiah Farmasi Imelda) 8(1), 92-101. https://doi.org/10.52943/jifarmasi.v8i1.1504.
Sathyaseelan, S., Rao, B., & Anushmati, S., 2024. Cosmeceuticals: A transit state from synthetic to natural. Indian Journal of Pharmacology 56, 42 - 51. https://doi.org/10.4103/ijp.ijp_244_21.
Strompfová, V., Štempelová, L., & Wolaschka, T., 2024. Antibacterial activity of plant-derived compounds and cream formulations against canine skin bacteria. Veterinary Research Communications 48, 1459 - 1470. https://doi.org/10.1007/s11259-024-10324-0.
Syafi, S., Pujiasmanto, B., Purwanto, E., & Suryanti, V., 2023. Phytochemical Screening of Tacca Plant (Tacca leontopetaloides L.) Ethanol Extract Using Spectrophotometry UV-VIS Method. International Journal on Advanced Science, Engineering & Information Technology 13(4).
Témoin-Fardini, S., Servant, J., & Sellam, S., 2017. New test method for the evaluation of the preservation efficacy of soaps at very alkaline pH made by saponification. International Journal of Cosmetic Science 39(5), 476-485. https://doi.org/10.1111/ics.12401
Vidal, N., Adigun, O., Pham, T., Mumtaz, A., Manful, C., Callahan, G., Stewart, P., Keough, D., & Thomas, R., 2018. The Effects of Cold Saponification on the Unsaponified Fatty Acid Composition and Sensory Perception of Commercial Natural Herbal Soaps. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry 23(9), 2356. https://doi.org/10.3390/molecules23092356
Widyaningsih, S., Chasani, M., Diastuti, H., & Rahmayanti, E., 2019. The storage time on the characteristic of liquid dishwashing soap from nyamplung seed oil (Calophyllum inophyllum L) and its antibacterial activity. IOP Conference Series: Materials Science and Engineering 509(1), 012131. https://doi.org/10.1088/1757-899X/509/1/012131
Wijekoon, M., Bhat, R., Karim, A., & Fazilah, A., 2013. Chemical Composition and Antimicrobial Activity of Essential Oil and Solvent Extracts of Torch Ginger Inflorescence (Etlingera elatior Jack.). International Journal of Food Properties 16(6), 1200-1210. https://doi.org/10.1080/10942912.2011.579674
Wilde, K., Nguyen, P., Whitten, D., & Canavan, H., 2017. Skin irritation testing of antimicrobial conjugated electrolytes. Biointerphases 12 2, 02C403. https://doi.org/10.1116/1.4979918.
Yadav, R., & Agarwala, M., 2011. Phytochemical analysis of some medicinal plants. The Journal of Phytology 3, 10-14.
Yan, Y., Xia, X., Fatima, A., Zhang, L., Yuan, G., Lian, F., & Wang, Y., 2024. Antibacterial activity and mechanisms of plant flavonoids against gram-negative bacteria based on the antibacterial statistical model. Pharmaceuticals 17(3), 292. https://doi.org/10.3390/ph17030292
Zdrada-Nowak, J., Aniołkowska, S., & Deska, M., 2025. The Evaluating Skin Acid–Base Balance After Application of Cold-Processed and Hot-Processed Natural Soaps: A Double-Blind pH Monitoring Study. Cosmetics 12, 120. https://doi.org/10.3390/cosmetics12030120.
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