The effect of long-term high-fat diet in ovariectomized Wistar rat (Rattus norvegicus)study on lipid profile, endothelial nitric oxide synthase(eNOS) dan endhotelin-1 (ET-1) serum

https://doi.org/10.19106/JMedSci005103201902

Tantri Analisawati Sudarsono(1*), Denny Agustiningsih(2), S Sunarti(3)

(1) Postgraduate Student of Medical Sciences, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
(2) Department of Physiology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
(3) Department of Biochemistry, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author

Abstract


Accumulation of cholesterol in the blood will cause stiffness in arteries and trigger the formation of atherosclerotic lesions. Estrogen has a role as an antioxidant that can prevent the low density lipoprotein(LDL) oxidation. In menopause with high-fat diet, the decrease of estrogen levels will trigger cholesterol accumulation in the blood lead to endothelial dysfunction mediated by endotelin-1 (ET-1) and nitric oxide synthase (eNOS). This study aimed to investigate the effect of long-term high-fat diet on the lipid profile, serum eNOS and ET-1levels on ovariectomized rat. It was experimental using 28 female Wistar rat divided into 4 groups. Group 1 was ovariectomized mice and given a standard diet (OVX-SD), Group 2 was ovariectomized mice and given a high-fat diet (OVX-HFD), Group 3 was not ovariectomized mice and given a standard diet(SHAM-SD) group, and Group 4 was not ovariectomized mice and given a high-fat diet (SHAM-HFD).Lipid profile of blood samples was measured pre- and post-treatment, whereas serum eNOS and ET-1 levels were measured post-treatment using ELISA method. No significantly difference of lipid profileon OVX-HFD group compared to that OVX-SD was observed. The serum eNOSlevel on OVX-HFD(702.11±68.73 pg/mL) was significantly lower than that OVX-SD (857.18±118.08 pg/mL) (p<0.05). However, there was no significantly different of serum ET-1 levelbetween OVX-HFD group (299.14±146.61 pg/mL) compared to that OVX-SD (194.25±102.96 pg/mL) (p>0.05). In conclusion, the serum eNOS levelon ovariectomized rat with long-term high-fat diet is lower than that on ovariectomized rat with standard diet.


Keywords


high - fat diet - ovacriectomy - lipid profile - endothelial nitric oxide synthase (eNOS) -endothelin-1 (ET-1)

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DOI: https://doi.org/10.19106/JMedSci005103201902

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