Antioxidant activity of mengkudu fruit juice (Morinda citrifolia) and the role as an inhibitor of advanced glycation and products (AGES) by glycosilation reaction
Eko Suhartono Eko Suhartono
(1*)
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(*) Corresponding Author
Abstract
Background: Mengkudu is a traditional plant with active compound that has antioxidant activity. Diabetes mellitus complication has causative link with AGEs formation that involves free radical. Objective: To know antioxidant activity of mengkudu and the role to inhibit AGEs formation. Methods: This experiment used pre and post test control group design. Antioxidant activity was deter-mined'by reacting the mengkudu juice with 2,4 dinitrophenylhydrazin (DNPH) and analyzed with spectro-photometer a.=390 nm. AGEs absorbancy was measured every 48 hours until 20 days with spectropho-tometer ?=340 nm. Dicarbonyl level was determined with DNPH method that was developed by Uchida and modified by Sadikin.
Result: Antioxidant activity of 0.25 g/ml mengkudu juice (Morinda citrifolia) was 125.46±27.79%.
Beside that, mengkudu juice can inhibit advanced glycation end products (AGEs) formation.
Conclusion: Mengkudu juice can decrease AGEs formation and has antioxidant activity.
Key words : antioxidant - mengkudu juice - AGEs - glycosilation
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Copyright (c) 2015 Eko Suhartono Eko Suhartono
This work is licensed under a
Creative Commons Attribution-NonCommercial 4.0 International License .
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