Organophosphate insecticide susceptible test and transovarial transmission detection of dengue virus on Aedes aegypti in Kendari

Abstract

Dengue Fever (DF) is a disease caused by the dengue virus that transmitted by Aedes aegypti
(Ae. Aegypti) and Ae. albopictus. Dengue fever is now one of the most important public health
problems in Indonesia. Vector control using insecticides is the most important strategy to control
the DF. Massal fogging and selective abatisation have implementedd intensively to control Ae.
aegypti. However after its a long time implementation, mosquitoes resistance and transovarial
transmission have been reported. The aim of the study was to evaluate the susceptibility status
of Ae. aegypti to organophosphate and its ability to transovarial transmit degue virus in Kendari
City, South East Sulawesi. This was a observational study using cross-sectional design conducted
in high endemic areas (Kadia Village) and low endemic areas (Kambu Village). Susceptibility
status of Ae. aegypti larvae from F1’s egg generation was evaluated by biochemic assay, whereas
the transovarial transmission of dengue virus of adult female Ae. aegypti was evaluated by
immunohistochemistry method using head squash preparation. The results showed that the Ae.
aegypti larvae resistant was higher in high endemic areas (Kadia Village) (83.33%) than in low
endemica areas (Kambu Village) (60.00%). In addition, transovarial transmission index (TTI) of
Ae. aegypti dengue virus in the high endemic areas (26%) was significantly higher than in the
low endemic areas (12%) (p<0.05). In conclusion, the Ae. aegypti larvae resitance to
organophosphate as well as the TTI in high endemic areas is higher than in low endemic area in
Kendari, Sout East Sulawesi.