Cytotoxicity of α-terpineol in HeLa cell line and its effects to apoptosis and cell cycle

Rasuane Noor(1*), Indwiani Astuti(2), . Mustofa(3)

(1) Program Studi Pendidikan Biologi, Fakultas Ilmu Pendidikan, Universitas Muhammadiyah, Metro
(2) Department of Pharmacology and Therapy, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta
(3) Department of Pharmacology and Therapy, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author


α-Terpineol is a natural compound of terpenoid alcohols class. However, it can be synthesized
from α-pinene of turpentin content. α-Terpineol has been reported as potential anticancer agent
due to its activity on inhibition of cells growth and induction of tumor cell death. However, its
anticancer activity in HeLa cervical cancer cells line has never been studied, yet. The aim of this
study was to evaluate the cytotoxicity of α-terpineol and its effects to apoptosis and cell cycle.
This was a quasi-experimental study with post-test only with non-equivalent control group
design. Cytotoxicity of á-terpineol was evaluated using MTT cell viability assay. The effect of α-
terpineol on cell apoptotis was tested using acridine orange-ethidium bromide staining method,
whereas its effect on cell cycle was evaluated by flowcytometry method. The results showed
that α-terpineol had cytotoxicity against HeLa cell with an IC50 value about 12.46 μg/mL.
Furthermore, α-terpineol induced the HeLa with an IC50 value about 13.12 μg/mL. Cell accumulation
at G1 phase during cell cycle after incubation with α-terpineol (52.78)was observed. In conclusion,
α-terpineol is potential as an anticancer due to its ability to induce cell apoptosis and to inhibit
the cell cycle at G1 phase.


-terpineol – cytotoxicity – apoptosis - cell cycle – anti cancer - HeLa


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