The effect of a-terpineol on cell cycle, apoptosis and Bcl-2 family protein expression of breast cancer cell line MCF-7

Damiana Sapta Candrasari; Sofia Mubarika; Mae Sri Hartati Wahyuningsih

doi:10.19106/JMedSci004702201502

The effect of a-terpineol on cell cycle, apoptosis and Bcl-2 family protein expression of breast cancer cell line MCF-7

https://doi.org/10.19106/JMedSci004702201502

Damiana Sapta Candrasari^(1*), Sofia Mubarika⁽²⁾, Mae Sri Hartati Wahyuningsih⁽³⁾

(1) Faculty of Pharmcy, Universitas Sanata Dharma,
(2) Department of Histology and Cell Biology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
(3) Department of Pharmacology and Therapy, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
(*) Corresponding Author

Abstract

ABSTRACT

The cytotoxic activity of a-terpineol on T47D and HeLa cancer cell lines have been reported. This study was conducted to evaluate the effect of a-terpineol on cell cycle, apoptosis and Bcl-2 as well as Bax expression on MCF-7 cell line. The cytotoxic activity of a-terpineol was determined using MTT cell assay. Cell cycle and apoptosis were analysed using flowcytometry, whereas Bcl-2 and Bax expression were evaluated using immunohistochemistry. The results showed that a-terpineol had cytotoxic effect on the MCF-7 cell lines with an IC50 value of 33.0 ± 5.4 μg/mL. a-Terpineol induced cell accumulation in Sub-G1 lead to apoptosis of the MCF-7 cell. Moreover, a-terpineol inhibited Bcl-2 and induced Bax expressions. In conclusion, a-terpineol has potential anticancer activity against MCF-7 cancer cell line trough through cells cycle inhibition and apoptosis stimulation.