Effect of Phosphate Fertilizer and Arbuscular Mycorrhizal Fungi on The Nutrient, Phosphateuptake and in Vitro Digestibility of Alfalfa


Bambang Suwignyo(1), Bela Putra(2*), Nafiatul Umami(3), Cahyo Wulandari(4), Ristianto Utomo(5)

(1) Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, Indonesia
(2) Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, Indonesia
(3) Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, Indonesia
(4) Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
(5) Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author


This study aimed to determine the effect of arbuscular mycorrhizal fungi (AMF) and phosphate (P) fertilizer on the nutrient content, phosphate uptake and in vitro digestibility of alfalfa (Medicago sativa L.).The research was conducted at green house of Forage and Pastures Science Laboratory, Faculty of Animal Science Universitas Gadjah Mada. The experiment was arranged in Completely Randomized Design using 3x4 factorial patterns with four replications. The first factor was dosage of phosphate fertilizer SP 36 (0, 60, and 120 kg/ha). Second factor was the dosage of AMF (0, 0.8, 1.6, and 2.4kg/ha). The variable measured was nutrient contents (crude protein, dry matter, and organic matter), total P uptake and dry matter and organic matter in vitro digestibility. The results showed that the interaction of AMF and P fertilizer had no significant effect on crude protein and total P uptake, but highly significant effect on the parameters of dry matter, organic matter and dry matter and organic matter in vitro digestibility. 


Alfalfa; AMF; Phosphate; Productivity; Regosol soil

Full Text:



Andrade, S.A.L. de., A.P.Jr., Domingues, and P. Mazzafer. 2015. Photosynthesis is induced in rice plants that associate with arbuscular mycorrhizal fungi and are grown under arsenate and arsenite stress. Chemosphere. 134:141–9.

AOAC. 2005. Official Method of Analysis of The Association of Official Analytical Chemists. Published by the Association of Official Analytical Chemists, Maryland.

Barret, G., C. D. Campbell, A. H. Fitter, and A. Hodge. 2011. The arbuscular mycorrhizal fungus Glomus hoi can capture nd transfer nitrogen from organic patches to its associated host plant at low temperature. Appl Soil Ecil. 48:102-105.

Barzin, H.B., M. F. Rayni, A. Sobhkhizi, and M. Noori. 2014. Effect of mycorrhiza fungi on percent of protein , plant height , dry weight , number of panicles in Triticum aestivum. Int J Biosci. 5:111–118.

Baslam, M., M. C. Antolin, Y. Gogorcena, F. Munoz, and N. Goicoechea. 2014. Changes in alfalfa forage quality and stem carbohydrates induced by arbuscular mycorrhizal fungi (AMF) and elevated atmospheric CO2. Ann Appl Biol. 164:190-199.

Correa, A., C. Cruz, and N. Ferrol. 2015. Nitrogen and carbon/nitrogen dynamics in arbuscular mycorrhiza: the great unknow. Mycorrhiza.

Cozzolino, V., M. Pigna, V. Di Meo, A. G. Caporale, and A. Violante. 2010. Effects of arbuscularmycorrhizal inoculation and phosphorus supply on the growth of Lactuca sativa L. and arsenic and phosphorus availability in an arsenic polluted soil under non-sterile conditions. Appl Soil ecol. 45:262-268.

Cruz, C., H. Egsgaard, C. Trujillo, P. Ambus, N. Requena, M. A. Martins-Loucao and I. Jakobsen. 2007. Enzymatic evidence for the key role of arginine in nitrogen translocation by arbuscular mycorrhizal fungi. Plant Physiol. 144:782-792.

Daynes, C.N., D. J. Field, J. A. Saleeba, M. A. Cole, and P. A. McGee. 2013. Development and stabilisation of soil structure via interactions between organic matter, arbuscular mycorrhizal fungi and plant roots. Soil Biol Biochem. 57:683–694.

Fellbaum, C. R., J. A. Mensah, A. J. Closs, G. E. Strahan, P. E. Pfeffer, E. T. Kiers, and H. Bucking. 2014. Fungal nutrient allocation in common mycorrhizal networks is regulated by the carbon source strength of individual host plants. New Phytol. 203: 646-656.

Fusconi, A. 2014. Regulation of root morphogenesis in arbuscular mycorrhizae: What role do fungal exudates, phosphate, sugars and hormones play in lateral rot formation? Ann Bot. 113:19-33.

Garrido, E., A. E. Bennett, J. Fornoni, and S. Y. Strauss. 2010. Variation in arbuscular mycorrhizal fungi colonization modifies the expression of tolerance to above-ground defoliation. J Ecol. 98:43–49.

Hawkins, H., A, Johansen, and E. George. 2000. Uptake and transport of organic and inorganic nitrogen by arbuscular mycorrhizal fungi. Plant Soil. 226: 275-285.

Jems, A.M., K. Maaruf, M. R. Waani, and C. J. Pontoh. 2014. Pengaruh Penggunaan Konsentrat dalam Pakan Rumput Benggala (Panicum maximum) terhadap Kecernaan Bahan Kering dan Bahan Organik pada Kambing Lokal. J zootek. 34:108–114.

Jin, H., J. Liu, J. Liu, and X. W. Huang. 2005. The uptake, metabolism, transport and transfer of nitrogen in an arbuscular mycorrhizal symbiosis. New Phytol. 168:687-696.

Jin. H. R., J. Liu, J. Liu, and X. W. Huang. 2012. Forms of nitrogen uptake, translocation, an transfer via arbuscular mycorrhizal fungi: A review. Sci China Life Sci. 55: 474-482.

Leifheit, E.F., S. D. Veresoglou, A. Lehmann, E. K. Morris, and M. C. Rillig. 2013. Multiple factors influence the role of arbuscular mycorrhizal fungi in soil aggregation—a meta-analysis. Plant Soil. 374:523–537.

Macolino, S., L. M. Lauriault, F. Rimi, and U. Ziliotto. 2013. Phosphorus and potassium fertilizer effects on alfalfa and soil in a non-limited soil. Agron J. 105:1613–1618.

Modupeola, T.O., J. O.Olaniyi, A. M. Abdul-Rafiu, O. O. Taylor, T. A. . Fariyike, and T. O. Oyebamiji. 2013. Effect of organic phosphorus fertilizer and plant density on the growth, yield and nutritional value of ginger (Zingiber officinale). Int J Agric Res. 8:94–100.

Parman, S. dan S. Harnina. 2008. Pertumbuhan, Kandungan Klorofil dan Serat Kasar pada Defoliasi Pertama Alfalfa (Medicago sativa L) Akibat Pemupukan Mikoriza. Bul Anat dan Fisiol. XVI:1–6.

Sabia, E., S. Claps, F. Napolitano, G. Annicchiarico, A. Bruno, R. Francaviglia, L. Sepe, and R. Aleandri. 2015. In vivo digestibility of two different forage species inoculated with arbuscular mycorrhiza in Mediterranean red goats. Small Rumin Res [Internet]. 123:83–87. Available from:http://linkinghub.elsevier.com/retrieve/pii/S0921448814002806.

Subantoro, R., P. Yudono, dan B. Suwignyo. 2013. Pertumbuhan dan Hasil Tiga Varietas Alfalfa (Medicago sativa L.) dengan Perlakuan Tiga Macam Rhizobium pada Media Tanam Regosol Asal Banguntapan. Jurnal Ilmu Pertanian. 15:69–84.

Tilley, J. M. A. dan R. A. Terry. 1963. A two-stage technique for in vitro digestion of forage crops. J. British Grassal. Soc. 28:104-111.

Veresoglou, S.D., B. Chen, and M. C. Rillig. 2012. Arbuscular mycorrhiza and soil nitrogen cycling. Soil Biol Biochem [Internet]. 46:53–62. Available from: http://dx.doi.org/10.1016/j.soilbio.2011.11.018.

DOI: https://doi.org/10.21059/buletinpeternak.v40i3.12401

Article Metrics

Abstract views : 2845 | views : 2757


  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Buletin Peternakan (Bulletin of Animal Science) Indexed by:

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.