Estimasi Sintesis Protein Mikrobia Rumen Menggunakan Ekskresi Derivat Purin dalam Urin dengan Teknik Spot Sampling pada Kambing Bligon dan Kambing Kejobong

Dianestu Putra; Lies Mira Yusiati; Ristianto Utomo

doi:10.21059/buletinpeternak.v40i3.12766

Estimasi Sintesis Protein Mikrobia Rumen Menggunakan Ekskresi Derivat Purin dalam Urin dengan Teknik Spot Sampling pada Kambing Bligon dan Kambing Kejobong

https://doi.org/10.21059/buletinpeternak.v40i3.12766

Dianestu Putra^(1*), Lies Mira Yusiati⁽²⁾, Ristianto Utomo⁽³⁾

(1) Faculty Animal Science Gadjah Mada University
(2) Faculty Animal Science Gadjah Mada University, Indonesia
(3) Faculty Animal Science Gadjah Mada University, Indonesia
(*) Corresponding Author

Abstract

This study were aimed to determine the correlation between concentration of purine derivatives (PD) in spot sample with PD total excretion in Bligon and Kejobong goats and determine the appropriate sampling time, in order to predicting microbial protein synthesis in both breeds. Six male Bligon goats and six male Kejobong goats with age range from 8 to 14 months and body weight from 16 to 21 kg were placed in metabolism cages. Peanut straw and water were given to both groups of goats through ad libitum feeding and drinking. The study was done in 14 days for adaptation, 3 days for collection. Sample of feeds, feed residues, and feces were collected and then analyzed to determine dry matter and organic matter content. Spot urine and the total daily urine samples were also collected. Samples collection of spot sampling technique was run by taking the urine periodically with 3 hours intervals at 24 hours. Urine samples were analyzed for the content of creatinine and PD which includes allantoin, uric acid, xanthine, and hypoxanthine. Data were tested for the correlation between concentration of PD spot urine sample with total PD daily excretion. It is known that the concentration of PD and creatinine (µmol/L) for Bligon were 1,418.40 and 202.85 respectively, while for Kejobong were 1,547.40 and 219.68 respectively. Total excretion of PD, allantoin, uric acid, xanthyne and hypoxanthine and creatinine (µmol/W0,75/day) for Bligon were 114.14, 95.86, 17.31, 0.97, and 16.40 respectively, with microbial protein synthesis efficiency was 4.61 g N/kg degraded of organic matter in rumen (DOMR). Total excretion of PD allantoin, uric acid, xanthyne and hypoxanthine and creatinine (µmol/W0,75/day) for Kejobong were 180.18, 158.17, 20.60, 1.40, and 24.87 respectively, with microbial protein synthesis efficiency was 6.90 g N/kg DOMR. Based on this study also known that the best time for spot sampling to determine the total excretion of PD in Bligon was in the range time of 11.00 am to 2.00 pm, with equation Y=1.474X+48.81, while Kejobong goat in the range of 2.00 to 5.00 pm, with equation Y=2.678X+5.692.

Keywords

Bligon goat; Kejobong goat; Purine derivative; Spot sampling

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References

Anonim. 2013. Fluitest Uric Acid. Analyticon® Biotechnologies AG. Lichtenfels.

AOAC. 2005. Official Method of Analysis of the Association of Official Analitycal Chemists. 18th edn. AOAC International. William Harwitz (ed). United States of America, Maryland.

Abdelatif, A. M., Salwa, A. Elsayed and Y. M. Hassan. 2010. Effect of state of hydration on body weight, blood constituents and urine excretion in nubian goats (Capra hircus). World J. Agric. Sci. 6: 178-188.

Andrade-Montemayor, H., T. G. Gasca and J. Kawas. 2009. Ruminal fermentation modification of protein and carbohydrate by means of roasted and estimation of microbial protein synthesis. R. Bras. Zootec. 38: 277- 291.

Astuti, D. A. and E. Wina. 2002. Protein balance and excretion of purine derivatives in urine of lactating Etawah Crossbred goats fed with tempe waste. Seminar Nasional Teknologi Peternakan dan Veteriner.

Astuti, M. 1980. Statistik. Bagian Pemuliaan Ternak Fakultas Peternakan Universitas Gadjah Mada, Yogyakarta.

Astuti, M., A. Agus, I. G. S. Budisatria, B. Aryadi, L. M. Yusiati, dan M. A. U. Muzayyanah. 2007. Peta Potensi Plasma Nutfah Ternak Nasional. Ardana Media, Yogyakarta.

Barbosa, A. M., R. F. D. Valdares, S. C. V. Filho, D. S. Pina, E. Detmann and M. I. Leao. 2011. Endogenous fraction and urinary recovery of purine derivatives obtained by different methods in Nellore Cattle. J. Anim. Sci. 89: 510- 519.

Basheir, R. A., S. A. Omer and O. S. A. Mohamed. 2009. Effect of lactation on some urine indices of renal functionin Nubian goats. J. Sci. Tech. 10: 1-8.

Belenguer, A., D. Yanez, J. Balcells, N. H. O. Baber and M. Gonza´lez-Ronquillo.2002. Urinary excretion of purine derivatives and prediction of rumen microbial outflow in goats. Livest. Prod. Sci. 77: 127-135.

Braga, J. M. D. S., R. F. D. Valadares, S. G. Pellizzoni, S. C. V. Filho, L. L. Prates and L. F. C. Silva. 2012. Estimation of endogenous contribution and urinary excretion of purine derivatives from the total digestible nutrient intake in Nellore heifers. R. Bras. Zootec. 41: 1899- 1906.

Chen, X. B. and E. R. Ørskov. 2003. Research on urinary excretion of purine derivatives in ruminant: Past present and future. International Feed Resources Unit. Macaulay Land Use Research Institute, Craigiebuckler, Aberdeen.

Chen, X. B. and M. J. Gomes. 1995. Estimation of microbial protein supply to sheep and cattle based on urinary excretion of purine derivatives. An overview of the technical details. Rowett Research Institute, Bucksburn. Aberdeen.

Chen, X. B., G. Grubic, E. R. Ørskov and P. Osuji. 1992. Effect of feeding frequency on diurnal variation in plasma and urinary purine derivatives in steers. Anim. Prod. 55: 185-191.

Chizzotti, M. L., S. C. V. Filho, R. F. D. Valadares, F. H. M. Chizzotti and L. O. Tedeschi. 2008. Determination of creatinine excretion and evaluation of spot urine sampling in Holstein cattle. Livest. Sci. 77: 127-135.

Csapo, Z. C. J., J. Schmidt and T. G. Martin. 2001. Quantitative determination of protein of bacterial origin. Trends in Analytical Chemistry. 20: 42-48.

Dipu, M. T., S. K. George, P. Singh, A. K. Verma and U. R. Mehra. 2006. Measurment of microbial protein supply in murrah buffaloes using urinary purine derivatives excretion and PDC index. Asian-Aust. J. Anim. Sci. 19: 347-355.

El-Nouty, F. D., A. A. Al-Haidary and S. M. Basmeil. 1990. Physiological responses, feed intake, urine volume and serum osmolarity of Aardi goats deprived of water during spring and summer. Asian-Aust. J. Anim. Sci. 3: 331-336.

George, S. K., M. T. Dipu, U. R. Mehra, A. K. Verma and P. Singh. 2006. Influence of level feed intake on concentration of purine derivatives in urinary spot samplings and microbial nitrogen supply in crossbred bulls. Asian-Aust. J. Anim. Sci. 19: 1291-1297.

Givens, D. I., E. Owen, R. F. E. Axford and H. M. Omed. 2000. Forage Evaluation in Ruminant Nutrition. CABI Publishing.

Kazemi-Bonchenari, M., K. Rezayasdi, H. A. Ghasemi, A. H. Farahani, M. Deghan- Banadaky and A. Mahdavi. 2011. Effect of rumen degradable protein suplementation on purine derivatives excreted through urine and milk in lactating Holstein cows. J. Anim. Vet. Adv. 10: 2389-2393.

Kertz, A. F., L. R. Prewitt, A. G. Lane and J. R. Campbell. 1970. Effect of dietary intake on creatinine excretion and the creatinine-nitrogen ratio in bovine urine. J. Anim. Sci. 30: 278-282.

Mbewe, M. R., V. R. Hamandishe, V. E. Imbayarwo-Chikosi and B. Masunda. 2014. Nitrogen balance and rumen microbial protein synthesis in goats fed diets containing soaked and roasted Mucuna bean (Mucuna pruriens).

Online J. Anim. Feed Res. 4: 06-09.Mota, M., J. Balcells, N. H. O. Baber, S. Boluktepe and A. Belenguer. 2008. Modelling purine derivative wxcretion in dairy goats: endogenous excretion and the relationship between deudenal input and urinary output. J. Anim. 2: 44- 51.

Nelson, D. L. and M. M. Cox. 2008. Principles of Biochemistry. 5th edn. Freeman Publishers, Wisconsins.

Prawirodigdo, S., T. Herawati, dan B. Utomo. 2008. Penampilan peternakan kambing dan potensi bahan pakan lokal sebagai komponen pendukungnya di wilayah Propinsi Jawa Tengah. Balai Pengkajian Teknologi Pertanian Jawa Tengah, Lokakarya Nasional Kambing Potong, pp: 157-163.

Purwati, C. S., L. M. Yusiati, dan S. P. S. Budhi. 2013. Kontribusi ekskresi basal purin terhadap total ekskresi derivat purin dalam urin kambing Bligon dan Kejobong. Buletin Peternakan 37: 6-11.

Ruiz, D. R. Y., A. I. M. Garcia, A. Moumen and E. M. Alcaide. 2004a. Ruminal fermentation and degradation patterns, protozoa population, and urinary purine derivatives excretion in goats and wethers fed diets based on olive leaves. J. Anim. Sci. 82: 3006-3014.

Ruiz, D. R. Y., A. Moumen, A. I. M. Garcia and E. M. Alcaide. 2004b. Ruminal fermentation and degradation patterns, protozoa population, and urinary purine derivatives excretion in goats and wethers fed diets based on two-stageolive cake: Effect of PEG supply. J. Anim. Sci. 82: 2023-2032.

Singh, M., K. Sharma, N. Dutta, P. Singh, A. K. Verma and U. R. Mehra. 2007. Estimation of rumen microbial supply using urinary purine derivatives excretion in crossbred calves fed at different levels of feed intake. Asian- Aust. J. Anim. Sci. 20: 1567-1574.

Utomo, B., T. Herawati and S. Prawirodigdo. 2005. Productivity of goat farming on rural condition. Seminar Nasional Teknologi Peternakan dan Veteriner, pp: 660-665.

Yusiati, L. M. 2005. Pengembangan metode estimasi sintesis protein mikrobia rumen menggunakan ekskresi derivat purin dalam urin berbagai ternak ruminansia Indonesia. Disertasi. Fakultas Peternakan, Universitas Gadjah Mada, Yogyakarta.

DOI: https://doi.org/10.21059/buletinpeternak.v40i3.12766