APPLICATION OF VINEGAR AS BIOPRESERVATIVE TO INHIBIT Salmonella typhimurium IN FRESH CHICKEN MEAT

https://doi.org/10.21059/buletinpeternak.v41i2.13596

Juniawati Juniawati(1*), Miskiyah Miskiyah(2), Widaningrum Widaningrum(3)

(1) Balai Besar Litbang Pascapanen Pertanian
(2) Balai Besar Litbang Pascapanen Pertanian
(3) Balai Besar Litbang Pascapanen pertanian
(*) Corresponding Author

Abstract


Using natural preservative is a choice amid fears consumers to use a chemical preservative in food products including fresh meat. Acetic acid can be used as a preservative because of its ability to inhibit the growth of microbes. However, a strong smell and taste of the synthetic acetic acid limit its use in food products. This research aims to determine the ability of acetic acid from banana peel vinegar and coconut water vinegar to inhibit the growth of Salmonella typhimurium in fresh meat. The study was conducted at two storage temperature are room temperature and temperature of 5-7°C where at each temperature is designed using completely randomized design with acid treatments (vinegar banana peel vinegar, coconut water vinegar, acetic acid and lactic acid commercial) and repeated in triplicates.  Sample 75 g that has been treated with acid soaking, inoculated with Salmonella, allowed until 20 minutes, packed and put at room temperature and refrigerated temperature.The result showed that banana peel vinegar is the most effective for reducing Salmonella typhimurium than coconut water vinegar, commercial acetic acid, and commercial lactic acid up to 24 hours at room temperature.  Coconut water vinegar is the most effective for reducing Salmonella typhimurium up to 12 days of storage at temperature 5-7°C.


Keywords


Acetic acid; Fresh meat; Salmonella; Vinegar

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References

Aftab, M., A. Rahman, M. S. Qureshi, S. Akhter, U. Sadique, A. Sajid, and S. Zaman. 2012. Level of Salmonella in beef of slaughtered cattle at peshawar. J. Anim. Plant Sci. 22: 24-27.

Alvarez-Ordonez, A., A. Fernandez, A. Bernando, and M. Lopez. 2009. Comparison of acids on the induction of an acid tolerance response in Salmonella typhimurium, consequences for food safety. Meat Sci. 81 : 65-70.

Andriani, Darmono, dan W. Kurniawati. 2007. Pengaruh asam asetat dan asam laktat sebagai antibakteri terhadap Salmonella sp yang diisolasi dari karkas ayam. Seminar Nasional Teknologi Peternakan dan Veteriner.

Braden, C. R. 2006. Salmonella enterica serotype Enteritidis and eggs: a national epidemic in the United States. Clinical Infection Disease. 43: 512-517.

Badan Standardisasi Nasional. 2009. Batas maksimum cemaran mikroba dalam pangan. SNI 7388:2009. Badan Standardisasi Nasional, Jakarta.

Bell, F. M., T. R.Marshall, and E. M. Anderson. 1986. Microbiological and sensory test of beef treated with acetic acid and formic acid. J. Food Protection 49: 207-210.

Budak, H. N., D. K. Dogue, C. M. Savas, A. C. Seydim, T. K. Tas, I. M. Ciris, and Z. B. Guzel-Seydim. 2011. Effect of apple cider vinegar produced with different techniques on blood lipids in high cholesterol fed rats. J. Agric. Food Chem. 59 : 6638-6644.

Burin, R. C. K., A. Silva Jr., and l. A. Nero. 2014. Influence of Lactic acid and acetic acid on Salmonella spp. Growth and expression of acid tolerance-related genes. Food Res. Int. 64: 726-732.

Dickson, J. S. and M. E. Anderson. 1992. Microbiological decontamination of food animal carcasses by washing and sanitizing systems: A review. J. Food Protection. 55: 133-140.

Dorsa, W. J., C. N. Cutter, and G. R. Siragusa. 1996. Evaluation of six sampling methods for recovery of bacteria from beef carcass surfaces. Food Microbiol. 22: 39-41.

Dorsa, W. J., C. N. Cutter, and G. R. Siragusa. 1998. Bacterial profile of ground beef made from carcass tissu experimentally contaminated with pathogenic and spoilage bacteria before being washed with hot water, alkaline solution, organic acids and then stored at 4oC or 120C. J. Food Protection. 6: 1109-1118.

Doughari, J. H. 2006. Antimicrobial activity of Tamarindus Indica Linn. Tropical J. Pharm. Res. 5: 597-603.

Goncalves, A. C., R. C. C. Almeida, M. A. O. Alves, and P. F. Almeida. 2005. Quantitative investigations on the effects of chemical treatments in reducing Listeria monocytogenes populations on chicken breast meat. Food Control. 16: 617-622.

Hanifah, R. 2013. Pemanfaatan cuka air kelapa untuk menghambat pertumbuhan bakteri pada daging sapi. Skripsi Fakultas Peternakan, Institut Pertanian Bogor, Bogor.

Hulankova, R. and G. Borilova. 2011. In vitro combined effect of oregano essential oil and caprylic acid against Salmonella sarovars, Escherichia coli O157:H7, Staphylococcus aureus and Listeria monocytogenes. Acta Veteriner. 80: 343-348.

Hutkins, E. W. and N. L. Nannen. 1993. pH homeostasis in lactic acid bacteria. J. Dairy Sci. 76: 2354-2365.

Jamilah, M. B., K. A. Abbas, and R. A. Rahman. 2008. A review on some organic acids additives as shelf life extenders of fresh beef cuts. American J. Agric. Biol. Sci. 3: 566-574.

Kwartiningsih, E. dan L. N. S. Mulyati. 2005. Fermentasi sari buah nanas menjadi vinegar. Ekuilibrium 4: 8-12.

Kotula, K. L. and A. W. Kotula. 2000. Microbial ecology of different types of food fresh red meats. In: The Microbiological Safety and Quality of Food. Lund, B. M., T. C. Baird Parker and G. W Gould (eds.). Aspen Publishers Inc, Gathersburg, MD, pp. 359-388.

Lawley, R., L. Cyrtis, and J. Davis. 2008. The Food Safety Hazard Guidebook. Cambridge: RSC Pub.

Leesmith, J. 2005. General Microbiology Laboratory. Kasetsart University Publishing. 58-60.

Mani-Lopez, E., H. S. Garcia and A. Lopez-Malo. 2012. Organic acids as antimicrobials to control Salmonella in meat and poultry products. J. Food Res. Int. 45: 713-72.

Mokbel, M. S. and F. Hashinaga. 2005. Antibacterial and antioxidant activities of banana (Musa, AAA cv. Cavendish) fruits peel. American J. Biochem. Biotechnol. 1: 125-131.

Nurliana, S. C. Yuda, F. Jamin, T. R. Ferasyil, M. Isa, dan Darmawi. 2015. Pengaruh pencelupan karkas ayam pedaging dalam larutan asam sitrat dan asam asetat terhadap angka lempeng total Escherichia coli. Jurnal Medika Veterinaria 9: 124-127.

Puspitasari, Sudrajat, dan E. Kusumawati. 2015. Efek penghambatan infusa rimpang lempuyang gajah terhadap angka cemaran bakteri pada daging ayam segar. Jurnal Science East Borneo 3: 17-21.

Rodriguez-Tudela, J. L., F. BArchiesi, J. Bille, E. Chryssanthou, M. Cuenca-Estrella, J. Denning, J. P. Donelly, B. Dupont, W. Fegeler, C. Moore, M. Richardson, and R. F. Verweij. 2003. Subcommittee on antifungal suspectibility testing of the ESCMID European Committee for Antimicrobial Suspectibility Testing (EUCAST). 9(8): 1-8.

Syarifah, I. dan E. Novarieta. 2015. Deteksi salmonella sp pada daging sapi dan ayam. Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner.

USDA. 2013. FSIS Compliance Guideline-HACCP Systems Validation. Washington DC, USA : USDA (http://www.fsis.usda.gov/shared/PDF/HACCP_systems_VAlidation.pdf).

Vergiyana, N., Rusman, dan Supadmo. 2014. Karakteristik mikroba dan kimia sosis ayam dengan penambahan khitosan dan angkak yang disimpan pada refrigerator. Buletin Peternakan. 38 :197-204.

Vipra, A., S. N. Desai, R. P. Junjappa, P. Roy, N. Poonacha, P. Ravinder, B. Sriram, and S. Padmanabhan. 2013. Determining the minimum inhibitory concentration of bacteriophage: Potential advantages. Adv. Microbiology. 3: 181-190.

Waghmare, J. S. and H. K. Ankeeta. 2014. GC-MS analysis of bioactive components from banana peel (Musa sapientum peel). Euro J. Experimental Biology. 4: 10-15.

Widaningrum, Miskiyah, dan Juniawati. 2015. Efikasi cuka kullit pisang dan air kelapa sebagai penghambat Listeria monocytogenes pada daging ayam. Jurnal Penelitian Pascapanen Pertanian 12: 93-104.

Zasshi K., E. Entani, M. Asai, S. Tsujihata, Y. Tsukamoto, and M. Ohta. 1997. Antibacterial action of vinegar against food borne pathogenic bacteria including Escherichia coli O157:H7 Part 1. Examination of Bacteriostatic and Bactericidal Activities. 71: 443-450.



DOI: https://doi.org/10.21059/buletinpeternak.v41i2.13596

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