MICROENCAPSULATION OF INDIGENOUS POULTRY LACTIC ACID BACTERIA PROBIOTIC ON THE COMPETITIVE EXCLUSION AGAINST Salmonella enteritidis AND Escherichia coli IN VITRO

Monica Sonia Indri Pradipta; Sri Harimurti; Widodo Hadisaputro

doi:10.21059/buletinpeternak.v41i2.17017

MICROENCAPSULATION OF INDIGENOUS POULTRY LACTIC ACID BACTERIA PROBIOTIC ON THE COMPETITIVE EXCLUSION AGAINST Salmonella enteritidis AND Escherichia coli IN VITRO

https://doi.org/10.21059/buletinpeternak.v41i2.17017

Monica Sonia Indri Pradipta^(1*), Sri Harimurti⁽²⁾, Widodo Hadisaputro⁽³⁾

(1) Universitas Gadjah Mada
(2) Universitas Gadjah Mada
(3) Universitas Gadjah Mada
(*) Corresponding Author

Abstract

This study was conducted to investigate the effect of microencapsulation of lactic acid bacteria (LAB) probiotic isolated from chickens’ gastrointestinal tract on Salmonella enterica serotype enteritidis ATCC 13076 and Escherichia coli EPEC. Probiotic of LAB used were Streptococcus thermophilus strain Kp-2, Lactobacillus murinus strain Ar-3, and Pediococcus acidilactici strain Kd-6. Microencapsulation were conducted by spray drying with inlet/outlet temperatures of 160/80°C using maltodextrin and skim milk powder (20% w/v) as coating materials. Competitive exclusion test was conducted in vitro using well diffusion method. Variable measured in this study was the clear zone observed. The data of clear zone among treatments were analyzed using analysis of variance (ANOVA) one way followed by Duncan multiple range test (DMRT); except the data of clear zone resulted by probiotic before and after microencapsulation that was analyzed using t-test. The result showed that the ability of each strain against pathogen was decreased after being encapsulated. S. thermophilus before and after microencapsulation had the same antagonistic ability against E. coli and S. enteritidis (P>0.05). Microencapsulation process with spray drying method decreased antagonistic ability of probiotic against pathogenic bacteria both in single and multi strain.

Keywords

Clear zone; In vitro; Microencapsulation; Probiotic; Well diffusion method

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References

Alakomi, H. L., E. Skytta, M. Saarela, T. Mattila-Sandholm, K. Latva-Kala, and I. M. Helander. 2000. Lactic acid permeabilizes Gram-Negative bacteria by disrupting the outer membrane. Appl Environ Microbiol. 66: 2001-2005.

Arias, O. A. B, M. de la Luz Reyes, M. L. Navarro, Y. B. Solis, M. Márquez, G. G. Sanchez, R. Snell, and R. Zuñiga. 2013. Antagonistic effect of probiotic strains against two pathogens: Salmonella typhimurium and E. coli O157:H7 resistant to antibiotics. E-Gnosis. 11: 1-16.

Behboudi-Jobbehdar, S., C. Soukoulis, L. Yonekura, and I. Fisk. 2013. Optimization of spray-drying process condition for the production of maximally viable microencapsulated L. acidophilus NCIMB 701748. Drying Technology. 31:1274-1283.

Berchieri, A., Jr., C. K. Murphy, K. Marston, and P. A. Barrow. 2001. Observation on the persistence and vertical transmission Salmonella enterica serovars pullorum and gallinarum in chickens: Effect of bacterial and host genetic background. Avian Pathol. 30: 221-231.

Burgain, J., C. Gaiani, M. Linder, and J. Scher. 2011. Encapsulation of probiotic living cells: from laboratory scale to industrial application. J. Food Eng. 104: 467-483.

Casey, P. G., G. E. Gardiner, G. Casey, B. Bradshaw, P. G. Lawlor, P. B. Lynch, F. C, Leonard, C. Stanton, R. P. Ross, G. F. Fitzgerald, and C. Hill. 2007. A five-strain probiotic combination reduces pathogen shedding and alleviates disease signs in pigs challenged with Salmonella enterica serovar typhimurium. Appl. Environ Microbiol. 73: 1858-1863.

Charkowski, A. O., J. D. Barak, C. Z. Sarreal, and R. E. Mandrell. 2002. Differences in growth of Salmonella enterica and Escherichia coli O157:H7 on alfalfa sprouts. Appl. Environ Microbiol. 68: 3114-3120.

Darabi, P., M. Goudarzvand, M. M. Natanzi, and Z. Khodaii. 2014. Antibacterial activity of probiotic bacteria isolated from broiler feces and commercial strains. Int. J. Enteric Pathog. 2:1-6.

Fuller, R. 1992. History and Development of Probiotics. In: Probiotics the Scientific Basis, Fuller, R (ed). Chapman & Hall, London, pp: 1-8.

Gardiner, G. E., P. G. Casey, G. Casey, P. B. Lynch, P. G. Lawlor, C. Hill, G. F. Fitzgerald, C. Stanton, and R. P. Ross. 2004. Relative ability of orally administered Lactobacillus murinus to predominate and persist in the porcine gastrointestinal tract. Appl Environ Microbiol. 70: 1895-1906.

Gharsallaoui, A., G. Roudaut, O. Chambin, A. Voilley, and R. Saurel. 2007. Applications of spray-drying in microencapsulation of food ingredients: an overview. Food Research International. 40: 1107-1121.

Gillor, O., A. Etzion, and M. A. Riley. 2008. The dual role of bacteriocins as anti- and probiotics. Appl Environ Microbiol Biotechnol. 81: 591-606.

Haider, M. G., M. G. Hossain, M. S. Hossain, E. H. Chowdhury, P. M. Das, and M. M. Hossain. 2004. Isolation and characterization of enterobacteria associated with health and disease in sonali chickens. Bangl. J. Vet. Med. 2: 15-21.

Hardewi, S. 2000. Potensi Bakteri Asam Laktat sebagai Agensia Probiotik Ayam: Ketahanannya terhadap Bile Salt dan Daya Hambat terhadap Patogen Salmonella sp. Skripsi Fakultas Teknologi Pertanian, Universitas Gadjah Mada, Yogyakarta.

Imagi, J., T. Yamanouchi, K. Okada, M. Tanimoto, and R. Matsuno. 1992. Properties of agents that effectively entrap liquid lipids. Bioscience, Biotechnology, Biochemistry. 56: 477-480.

Kabir, S. M. L. 2010. Avian colibacillosis and salmonellosis: a closer look at epidemiology, pathogenesis, diagnosis, control and public health concerns. Int. J. Environ. Res. Public Health. 7: 89-114.

Kailasapathy, K. 2002. Microencapsulation of probiotic bacteria: technology and potential applications. Curr Issues Intest Microbiol. 3: 39-48.

Kizerwetter-swida, M. and M. Binek. 2005. Selection of potentially probiotic Lactobacillus strains towards their inhibitory activity against poultry enteropathogenic bacteria. Pol. J. Microbiol. 54: 287-294.

Kusumaningsih, A., Supar, M, Sudarwanto, I. W. T. Wirawan, dan T. Ariyanti. 2007. Profil resistensi terhadap antimikroba isolat Salmonella enterica serotipe enteritidis yang diisolasi dari ayam dan produknya. Prosiding Seminar Nasional Hari Pangan Sedunia XXVII. Bogor, Indonesia, 21 November 2007.

Mortazavian, A. M., R. Mohammadi, and S. Sohrabvandi. 2012. New Advances in the Basic and Clinical Gastroenterology: Delivery of Probiotic Microorganisms into Gastrointestinal Tract by Food Products. Iran: Tehran. www.intechopen.com. Diakses pada 7 Januari 2015.

Muttaqin, A. 2005. Mempelajari pembuatan pelet probiotik bakteri asam laktat untuk suplemen pakan ternak ayam. Skripsi. Jurusan Teknologi Pangan dan Hasil Pertanian Fakultas Teknologi Pertanian. Universitas Gadjah Mada, Yogyakarta.

Mutukumira, A. N., J. Ang, and S. J. Lee. 2014. Viability and properties of spray-dried Lactobacillus casei-01. Proceedings of International Conference on Beneficial Microbes. Penang, Malaysia, 27^th-29^th May 2014.

Papaconstantinou, H. T. and S. Thomas. 2007. Bacterial Colitis. Clin Colon Rectal Surg. 20: 18-27.

Ray, B. 2004. Fundamental Food Microbiology. United States of America, CRC Press, Inc., Washington, D.C.

Schillinger, U. and F. K. Lucke. 1989. Antibacterial activity of Lactobacillus sake isolated from meat. Appl Environ Microbiol Biotechnol. 55: 1901-1906.

Serna-Cock, L. and V. Vallejo-Castillo. 2013. Probiotic Encapsulation. Afr. J. Microbiol. Res. 7: 4743-4753.

Setyaningrum, S. 2000. Potensi bakteri asam laktat sebagai agensia probiotik ayam: daya hambat terhadap patogen E. coli dan ketahanannya terhadap kondisi asam. Skripsi Fakultas Teknologi Pertanian, Universitas Gadjah Mada, Yogyakarta.

Shah, D. H., C. Casavant, Q. Hawley, T. Addwebi, D. R. Call, and J. Guard. 2012. Salmonella enteritidis strains from poultry exhibit differential responses to acid stress, oxidative stress, and survival in the egg albumen. Foodborne Pathog Dis. 9: 258-264.

Harimurti, S., E. S. Rahayu, Nasroedin, and S. Kurniasih. 2007. Lactic acid bacteria isolated from the gastro-intestinal tract of chicken. Anim Prod. 9: 82-91.

Harimurti, S. 2009. Study of competitive exclusion of Salmonella pullorum by probiotic strains in the broiler chickens. Proceedings of International Seminar of the 5^th Asian Conference on Lactic Acid Bacteria: Microbes in Disease Prevention and Treatment. Singapore, July 2009.

Harimurti, S. 2011. Probiotik bakteri asam laktat indigenous: pengaruhnya terhadap ekspresi biologis pada ayam broiler. Tesis. Sekolah Pascasarjana, Universitas Gadjah Mada, Yogyakarta.

Walter, J. 2008. Ecological role of lactobacilli in the gastrointestinal tract: implications for fundamental and biomedical research. Appl. Environ Microbiol. 74: 4985-4996.

DOI: https://doi.org/10.21059/buletinpeternak.v41i2.17017