EFFECT OF LIGNOSELULOLITIC FUNGUS TO ENZIMATIC ACTIVITY, FIBER FRCTION, AND DIGESTIBILITY ON FERMENTATION PROCESS OF COCOA POD

https://doi.org/10.21059/buletinpeternak.v41i3.22657

Engkus Ainul Yakin(1*), Zaenal Bachruddin(2), Ristianto Utomo(3), Ria Millati(4)

(1) Departement of Animal Science Faculty of Agriculture Universitas Veteran Bangun Nusantara
(2) Faculty of Animal Sciences, Universitas Gadjah Mada Yogyakarta
(3) Faculty of Animal Sciences, Universitas Gadjah Mada Yogyakarta
(4) Faculty of Food and Agriculture Product Technology, Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author

Abstract


The study was conducted to determine the enzyme activity, fiber fraction and digestibility in the fermentation process of cocoa pod. The substrate was used the cocoa pod while the fungi used Phanerochaete chrysosporium, Pleurotus ostreatus and Schizophyllum commune. Preparation of cocoa pod was chopped, finely ground and then dried. Preparation of fungi by growing fungi in liquid medium. Research methodology was the fermentation conducted with different fungi used four treatments and five replications. T1 = fermentation of cocoa pod without fungi addition, T2 = fermentation of cocoa pod with P. chrysosporium addition, T3 = fermentation of cocoa pod with  P. ostreatus addition, and T4 = fermentation of cocoa pod with S. commune. Fermentation used Erlenmeyer 250 ml and weight of cocoa pod was 100 gram. Fungi added on 5% from substrat weight bassis dry matter. Variables observed was enzyme activity, fiber fraction and digestibility . This study was designed using research design completely randomized design with a unidirectional pattern analysis of variance. Significant variables followed Duncan’s multiple range test. The results showed fermentation used P. chrysosporium has highest lignin peroxidase enzyme activity of 0.52±0.04 U/mL and mangan peroxidase 0.06±0.00 U/mL, neutral detergent fiber 75,54±0,41%, acid detergent fiber 68,10±0,30%, lignin 26,86±0,19%, cellulose 27,17±0,25%, hemicellulose 6,77±0,52%, dry matter digestibility 69,70±0,43% and organic matter digestibility 69,59±1,03%. The conclusion from this research that the fermentation by using fungi P. chrysosporium addition has the best result to degradated lignin. 


Keywords


Cocoa pod, Digestibility, Enzyme activity, Fermentation, Fiber fraction

Full Text:

PDF



DOI: https://doi.org/10.21059/buletinpeternak.v41i3.22657

Article Metrics

Abstract views : 773 | views : 579

Refbacks

  • There are currently no refbacks.




Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Buletin Peternakan (Bulletin of Animal Science) Indexed by:

 Harvard Library
Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.