THE PROPERTIES OF EDIBLE FILM DERIVED FROM BOVINE SPLIT HIDE GELATIN WITH ISOLATED SOY PROTEIN USING VARIOUS LEVELS OF GLYCEROL IN THE PRESENCE OF TRANSGLUTAMINASE

https://doi.org/10.21059/buletinpeternak.v41i3.24329

Dwi Wulandari(1*), Yuny Erwanto(2), Yudi Pranoto(3), Rusman Rusman(4)

(1) Politeknik ATK Yogyakarta
(2) Universitas Gadjah Mada
(3) Universitas Gadjah Mada
(4) Universitas Gadjah Mada
(*) Corresponding Author

Abstract


This study examined the characteristics of the edible film mixture gelatin bovine split hide and soy protein isolate, cross-linked using transglutaminase enzyme with addition level of glycerol as a plasticizer.The material used gelatin  combination (Gelatin bovine split hide: soy proteinisolate = 90: 10 with 30 Utransglutaminase enzyme levels ), and glycerol. Data were analyzed  using completely randomized design each treatment was replicated thrice of glycerol as a treatment level ( 10%, 20% dan 30%). The results showed that the glycerol level significantly affected the thickness, elongation, solubility, degree of cross-linking and WVTR, but not the tensile strength.The addition of glycerol causing the film becomes more elastic and  the surface is more homogeneous and compact. Increasing the level to 30% glycerol affects the mechanical properties of an edible film.

Keywords


Bovine split hide, Edible film, Enzyme transglutaminase, Gelatin, , Soy Protein Isolate

Full Text:

PDF


References

Abdel-Mohzen, A.M, A.S. Aly, R. Hrdina , A.S. Montaser and A. Hebeish. 2001. Eco-Synthesis of PVA/chitosan hydrogels of biomedical aplication. J. Polym. Environ. 19 : 1005 -1012. Anonimous, 2004. Glycerol, Related : Organic Chemistry. http://www. Encyclopedia.com. Accessed on Juny 13, 2014. ASTM. 1995. Standard Test Method For Water Vapor Transmission Of Materials E 96-95. In R. A. Storer (Ed.), Annuals Book Of ASTM Standards.). Philadelphia, PA: American Society for Testing and Materials. pp. 697–704. Bourtoom. 2008. Edible film and coatings characteristics and properties. International J.Food Resour.15 (3) : 1- 9. Carvalho, R. A. and C. R. F. Grosso. 2004. Characterization of gelatin based films modified with transglutaminase, glyoxal and formaldehyde. Food Hydrocolloids. 18 :717–726. Chambi, H. and C. Grosso. 2006. Edible film produced with gelatin and casein cross-linked with transglutaminase. International J. Food Res. 39 : 458- 466. Chen, H. 2002. Formation and properties of casein films and coatings. In A. Gennadios (Ed.), Protein-Based Films And Coatings. Boca Raton, FL: CRC Press, pp. 181–211. Cho, S. Y. and C. Rhee. 2004. Sorption characteristics of soy protein film and the irrelation to mechanica properties. Lebensmittle –Wissenschaft und-Technology. 35 (20):151- 157. De Jong, G. A. H. and S. J. Koppelman. 2002. Transglutaminase catalyzed reactions: impact on food applications. J. Food Sci. 67: 2798–2806. Gennadios, A, J. M. Rhim, A. Handa, CL Weller and MA Hanna. 1998. Ultraviolet radiation affects physical and molecular properties of soy protein films. J.Food Sci. 63 : 559- 563. Gomez-Guillen, M. C, A. I. Sarabia, M. T. Solas and P. Montero. 2001. Effect of microbial transglutaminase on the functional properties of megrim (Lepidorhombus boscii) skin gelatin . J. Sci Food Agric. 81 (7) : 665- 673. Gontard, N, S. Guilbert, and J. L.Cuq. 1993. Water and glyserol as plasticizer afect mechanical and water barrier properties of an edible wheat gluten film. J. Food Sci. 58 (1): 206 - 211. Hasdar. 2011. Characterization of Edible Film Produced From A Combination of Chicken Legs Skin Gelatin and Soy Protein Isolates. Thesis .Faculty of Animal Husbandry of Gadjah Mada University, Yogyakarta. Hastutiningrum, S. 2009. Utilization waste split leather tanning industry to glue the hydrolysis of collagen. J. Technol. 2 (2) : 208-212. He-Tang, C, Y. Jiang, Q. B. Web and X. Q.Yang. 2005. Effect of transglutaminase treatment on the properties of cast film of soy protein isolates. J. Biotechnol. 120 : 296-307. Hennik, W. E. and C. F. van Nostrum. 2002. Novel crosslinking methods to design hydrogels. Advanced dry delivery. Reviews. 15 : 13 – 36. Kim, K. W., C. J. Ko and H. J. Park. 2002. Mechanial properties, water vapor permeabilities and solubilities of highly carboxymethylated starch-based edible films. J.Food Sci. 67 (1) : 218-222. Kim, Y. T. 2006. Development and Characterization of Gelatin Film Asa Active Packaging Layer. Clemson: Clemson University. Mendieta-Taboada, O, P. Jose do, A. Sobral, R. A.Carvalho, A. Monica and B. Q. Habitante.2007. Thermomecanichal properties of biodegradable films based on blends of gelatin and poly (vinyl alcohol). Food Hydrocolloids. 22 ( 8) : 1458-1492. Mariniello, L, P. Di Pierro, C. Esposito, A. Sorrentino, P. Masi and R. Porta. 2003. Preparation and mechanical properties of edible pectin-soy flour films obtained in the absence or presence of transglutaminase. J. Biotechnol. 102: 191–198. Motoki, M., H. Aso, K. Seguro, and N. Nio. 1987. as1-casein film prepared using transglutaminase. Agr Biol Chem. 51: 993–996. Navarro-Taragaza, M. L., R. Sothornvit, and M. B.Perez-Gago. 2008. Effect of plasticizer tyoe and amount on hydroxypropyl methylcellulose-beeswax edible film properties and postharvest quality of coated plums (Cv. Angeleno). ASAP. J. Agr Food Chem. 32 (2) : 223-228. Nieto, M. B. 2009. Structure and fFunction of Polysaccharide Gum-Based Edible Films and Coatings for Food Applications, ed. Me embuscado and kc Huber. 57-112. New York: Springer Orliac, O, A. Roully, F .Silvestre, and L. Rigal. 2002. Effects of additives on the mechanical properties, hydrophobicity and water uptake of thermo-moulded films produced from sun flower protein isolate. Polymer. 43 : 5417–5425. Perez, M, P. Montero, and M. C.Gomez. 2007. Formulation and stability of biodegradable film made from cod gelatin and sun fower oil blends. Food Hidrocolloids. 1- 31. Reed,T, A. H. Barret, J. Briggs, and M. Richardson. 1998. Texture and storage stability of processed beef sticks as affected by glycerol and moisture levels. J. Food Sci. 63 : 84- 87. Rodriquez, M., J. Ose’s, K. Ziani, and J. I.Mate. 2006. Combined effect of plasticizers and surfactans on the physical properties of starch based edible film. Food Res Int . 39 : 840-846. Said, M. I. 2011. Optimization of The Production Process Goat Skin Gelatin Edible Film as A Raw Material for Drug Packaging Material (Capsule). Dissertation. Graduate Program Faculty of Animal Husbandry, Gadjah Mada University, Yogyakarta. Shaw, N. B, A. O. Sullivan, K. Hoffmann, and J. P. Kerry. 2003. Edible film from fish collagen – the effect of species and fishing grounds on their physical properties. http://www.rf.15/taft2003/Poster/20abstracts/shawcollagen.pdf. Accessed on Oktober 8, 2013. Sobral, P. A. J, F. C. Menegalli, M. D. Hubinger and M. A. Roques. 2001. Mechanical water vapor barrier and thermal properties of gelatin based edible film. Food Hidrocolloids. 15 : 423 – 432. Sompie, M. 2014. Characterization of Edible Films Made Basic Pig Skin Gelatin as Pork packers. Dissertation. Post graduate program Faculty of Animal Husbandry, Gadjah Mada University, Yogyakarta. Steel, R. G. and J. H. Torrie. 1993. Principle and Procedure of Statistic. Mc. Graw Hill Book Compani. Inc New York. Suharjito. 2007. Potential and prospects of industrial gelatin from bovine split hide. Journal of Sci Tech Plan. 5 (1) :43-48. Thomazine, M., R. A. Carvalho and P. J. A. Sobral. 2005. Physical properties of gelatin films plasticized by blends of glycerol and sorbitol. J. Food Sci. 70 : 172–176. Wulandari, D, S. Triatmojo, Y. Erwanto and Y. Pranoto. 2016. Physicochemical properties and amino acid and fuctional group profiles of gelatin ectracted from bovine split hide cured by acid. Pakistan J Nut. 15 (7): 655-661. Yi, J. B. , Y. T. Kim, H. J. Bae, W. S. Whiteside, and H. J. Park. (2006). Influenche of transglutaminase-induced cross-linking on properties of fish gelatin film. J.Food Eng Phy Prop. 71 (9) : 376-383. Yildirim, M. and N. S. Hettiarachchy. 1998. Properties of films produced by cross-linking whey proteins and 11S globulin using transglutaminase. J Food Sci. 63: 248–252. Yokoyama, K., N. Nio, and Y. Kikuchi. 2004. Properties and applications of microbial transglutaminase. Appl Microbiol biot. 64: 447-454. Zhang,Y. and J. H. Han. 2006. Plasticization of pea starch film with monosacharides and polyols. J. Food Sci. 71 (6) : 253- 261.



DOI: https://doi.org/10.21059/buletinpeternak.v41i3.24329

Article Metrics

Abstract views : 3284 | views : 2740

Refbacks

  • There are currently no refbacks.




Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Buletin Peternakan (Bulletin of Animal Science) Indexed by:

   
 
Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.