THE EFFECT OF ALPHA LIPOIC ACID ON BLASTOCYST EMBRYO DEVELOPMENT IN VIVO IN MICE (Mus musculus) EXPOSED CIGARETTE SMOKE

https://doi.org/10.21059/buletinpeternak.v41i4.24701

Syahruddin Said(1*), Adi Setyawan Prianto(2), Senri Utama Pramadipta(3)

(1) Research Centre For Biotechnology, Indonesian Institute of Sciences
(2) Faculty of Medical, Jenderal Soedirman University
(3) Faculty of Medical, Jenderal Soedirman University
(*) Corresponding Author

Abstract


This study was conducted to find out effect of alpha lipoic acid (ALA) on the development of in vivo blastocyst embryo in mice (Mus musculus) exposed in cigarettes smoke. This study used 30 mice divided into 6 treatment groups factorial 2x3. Factor A is treatment of ALA (0; 16.5 μM; 49.5 μM) per orally. Factor B is the treatment of cigarette smoke (with and without cigarette smoke). Giving cigarette smoke was done by covering the cage with plastic having two holes for cigarette smoke and airflow. The data obtained were analyzed using a complete randomized design (RAL) 2x3, followed by parametric Analysis of Variance (ANOVA) and independent sample T-Test, and Post Hoc Duncan test. The development of embryo of mice reaching blastocyst stage at ALA with dose 16,5 μM (16.00±7.12) were significantly higher (p<0.05) than negative control (13.50±1.73), but no significant effect with ALA dose 49.5 μM (19.25±4.03). The development of mice blastocyst embryos exposed to cigarette smoke (7.25±2.99) were significantly lower (p<0.05) compared with negative control. When the mice exposed to cigarette smoke were given ALA, the development of the blastocyst embryo returned to normal as in control, where the doses of 16.5 μM and 49.5 μM were not significantly different. It can be concluded that (1) ALA dose 16.5 μM and 49.5 μM have positive effect on blastocyst development in vivo, (2) cigarette smoke have negative effect on blastocyst development, (3) ALA 16.5 μM and 49.5 μM per orally capable counteract the oxidative stress caused by exposure to cigarette smoke.


Keywords


ALA; Blastocyst; Cigarette smoke; Development embryos; In vivo; Mice

Full Text:

PDF


References

, I. B. P. 2012. Perbedan Kadar Serum Glutathione Peroxidase (Gpx) pada Blighted Ovum dan Kehamilan Normal. Universitas Udayana, Denpasar.

Ali, A. A., J. F. Bilodean, and M. A. Sirard. 2003. Antioxidant requirements for bovine oocytes varies during in vitro maturation, fertilization, and development. Theriogenology 59: 939-949.

Anggraini, D. 2006. Pengaruh pemberian vitamin E terhadap motilitas spermatozoa mencit jantan strain BALB/C yang diberi paparan asap rokok. Skripsi. Fakultas Kedokteran Universitas Diponegoro, Semarang.

Bordel, R., M. W. Laschke, M. D. Menger and B. Volmar. 2006. Nicotine does not effect vascularization but inhibit growth of freely transplanted ovarian follicles by Inducing Granulosa Cell Apoptosis. Hum. Reprod. 21 (3)g::enase Bradyrhizobium j: 610-616.

Cakatay, U. 2006. Pro-oxidant Actions of Alpha Lipoic Aid and Dihydriolipoic Acid. Med. Hypothesis 66: 110-117.

Campbell, A., J. B. Neil, Reece and L. G. Mitchell. 2004. Biologi. Edisi kelima Jilid III. Diterjemahkan oleh: Prof. Dr. Ir. Wasmen Manalu. Erlangga, Jakarta.

Card, J. P. and J. A. Mitchell. 1979. The effects of nicotine on implantation rat. Biol. Reprod. 20: 532-539.

Carlson, D. A., A. R. Smith, S. J. Fisher, K. L. Young and L. Packer. 2007. The plasma pharmacikinetics of R(+) lipoic acid administered as sodium (R+) lipoate to healthy human subjects. Alter. Med. Rev. 12: 343-351.

Claudia, V., E. D. Queljoe and L. Tendean. 2013. Perbedaan kualitas spermatozoa mencit jantan (Mus Musclus L) yang diberikan vitamin C setelah pemaparan asap rokok. J. e-Biomedik, 1: 1.

Dahlan, S. 2011. Statistik untuk Kedokteran dan Kesehatan. Edisi 5. Salemba Medika, Jakarta.

Feugang, J. M., O. Camargo-Rodríguez and E. Memili. 2009. Culture systems for bovine embryos. Lives. Sci. 121:141-149.

Gannon, A. M. 2013. Expossure to cigaratte smoke and its impact on the ovarian follicle population: mechanisms of follicle loss. Thesis. Mc Master University, Canada.

Goraca, A., H. Huk–Kolega, A. Piechota, P. Kleniewska, E. Ciejka and B. Skibska. 2011. Lipoic Acid-Biological Actvity and Therapeutic Potential. Pharm. Rep. 63: 849-858.

Gupta, S., L. Sekhon, Y. Kim and A. Agarwak. 2010. The role of antioxidant stress and antioxidant in assisted reproduction. Woman’s Health Rev. 6: 227-238.

Harris, S. 2002. Experimental and clinical investigation into mammalian oocyte metabolism, nutrition, and fertility. Dissertation. University of Leeds.

Hatami, S., S. Zavareh, M. Salehnia, T. Lashkarbolouki, M. T. Ghorbanian and I. Karimi. 2014. The impact of alpha lipoic acid on developmental competence of mouse vitrified pre-antral follicles in comparison to those isolated from vitrified ovaries. Iran J. Reprod. Med. 12: 57-64.

Johnson, M., E. Freeman, D. Gardner and P. Hunt. 2007. Oxidative metabolism of pyruvate is required for meiotic maturation of murine oocyte in vivo. Biol. Reprod. 77: 2-8.

Kim, D. C., D. W. Jun, E. C. Jang, E. Kim, S. P. Lee, K. N. Lee, and H. L. Lee. 2013. Lipoic acid prevents the changes of intracellular lpid partitioning by free fatty acid. Gut. and Liv. 7: 221-227.

Kitagawa, Y., K. Suzuki, A. Yoneda and T. Watanabe. 2004. Effects of oxygen concentration and antioxidants on the in vitro developmental ability, production of reactive oxygen species (ROS) and DNA fragmentation in Porcine embryos. Theriogenology 62: 1186-97.

Marhaeni, G. A. 2009. Paparan Asap Rokok Menghambat Folikulogenesis Dan Perilaku Seksual Mencit. Thesis. Universitas Udayana, Denpasar.

Medicinus. 2011. Anti aging. Scientific J. Pharm.  Develop.  Med. Appl. 2: 5-7.

Moini, H., L. Packer and N. E. Saris. 2002. Antioxidant and prooxidant activities of alpha lipoic acid and dihydrolipoic acid. Toxicol. Appl. Pharmacol. 96: 41-48.

Molyneux, S. L., C. M. Florkowski and P. M. George. 2008. Koenzim q10 an independent predictor of mortality in chronic heart failure. J. Am. Coll. Cardiol. 52: 1435-1441.

Neganova, I. G., G. Sekirina and U. E. Ritter. 2000. Surface-expressed E-cadherin, and mitochondrial and microtubule distribution in rescue of mouse embryos from 2-cell block by Aggregation.  Mol. Hum. Reprod. 6 : 454-46.

Oktavianis. 2011. Efek pemberian asap rokok terhadap kehamilan tikus putih (Rattus norvegicus). Tesis. Program Studi Ilmu Biomedik Program Pascasarjana Universitas Andalas, Palembang.

Paine, M. A., E. H. Ruder, T. J. Hartman, J. Blumberg and M. B. Goldman. 2013. Chapter 4. Oxidatie Stress, Oogenesis and Folliculogenesis, In: Studies on Women’s Health. A. Agarwal, N. Aziz, B. Rizk (Ed.). Oxidative Stress ini Applied Basic Research and Clinical Practice. Springer Science Business Media, New York.

Said, S., O, P. Astirin, dan S. Wahyuningsih. 2011. Tingkat fertilitas dan perkembangan embrio mencit yang diberi ekstrak buah merah. Media Peternakan 6: 112-116.

Shay, K. P., R.F. Moreau, E. J. Smith, A. R. Smithand T.M. Hagen. 2009. Alpha- Lipoic Acid as A Dietary Supplement : Molecular Mechanisms and Therapeutic Potential. Biochem. Biophys. Acta. 1790 (10) : 1149-1160

Shiloh, H., R. L. Baratz, M. Koifman, D. Ishai, D. Bidder, Z. Wener-Meganzi and M. Dirnfeld. 2004. The impact of cigerette smoking on zona pellucida rhicjness of oocyte and embryos prior to transfer into uterine cavity. Hum. Reprod. 19: 157-159.

Sumiati. 2012. Pengaruh nikotin terhadap kadar malonildialdehid (MDA) serum dan keberhasilan fertilitas in vitro pada Rattus norvegicus di FK Unair Surabaya. Embrio Jurnal Kebidanan 1: 1-4.

Talebi, A., S. Zavarch, M. H. Kashani, T. Lashgarbluki and I. Karimi. 2012. The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles. J. Ass. Reprod. Gen. 29: 175-183.

Utami, T. S., R. Arbianti, H. Hermansyah and A. Reza. 2009. Perbandingan aktivitas antioksidan ekstrak etanol daun simpur (Dillenia indica) dari berbagai metode ekstraksi dengan uji ANOVA. Seminar Nasional Teknik Kimia Indonesia, 19-20.

Valavanidis, A., T. Vlachoggiani and K. Fiotakis. 2009. Tobacco smoke: involvement of reactive oxygen species and stable free radicals in mechanisms of oxidative damage carcinogenesis and synergistic effects with other respirable particles. Int.  J. Environ. Res. Public Health 6: 445-462.

Winarsi, H. 2007. Antioksidan Alami dan Radikal Bebas: Potensi dan Aplikasinya dalam Kesehatan. Penerbit Kanisius, Yogyakarta.



DOI: https://doi.org/10.21059/buletinpeternak.v41i4.24701

Article Metrics

Abstract views : 1102 | views : 1016

Refbacks

  • There are currently no refbacks.




Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Buletin Peternakan (Bulletin of Animal Science) Indexed by:

 Harvard Library
Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.