Antioxidant Enzyme Activity and Malondialdehyde Concentration on Broiler Fed Contain Lauric Acid and Areca vestiaria Giseke
Jola Josephien Mariane Roosje Londok(1*), Sumiati Sumiati(2), I Komang Gede Wiryawan(3), Wasmen Manalu(4)
(1) Department of Animal Nutrition and Feed, Faculty of Animal Husbandry, Sam Ratulangi University, Manado, 95115 , Indonesia
(2) Departement of Nutrition and Feed Technology, Bogor Agricultural University, Bogor, 16680, Indonesia
(3) Departement of Nutrition and Feed Technology, Bogor Agricultural University, Bogor, 16680, Indonesia
(4) Department of Anatomy, Physiology, and Pharmacology, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, 16680, Indonesia
(*) Corresponding Author
Abstract
The objective of the study was to determine antioxidant enzyme activity and concentration of malondialdehyde on broiler which consumed feed containing lauric acid and natural antioxidant from Areca vestiaria Giseke. The study used 240 day-old chicks of Lohmann MB 202 P strain placed randomly at 24 experimental cage units (10 chickens each) of the litter system. The average temperature of the cage at the study was 25.80 to 32.080 C with relative air humidity rate of 67.69 to 86.20%. The research method followed a Factorial Completely Randomized Design 2 x 4 with 3 replications. The first factor was the source of lauric acid in feed, i.e. conventional coconut oil (CO) and pure lauric acid (LA). The second factor was the source of antioxidant in the form of dosage of Areca vestiaria (AV) and vitamin E supplementation (TF) with four levels of AV, ie 0, 625 mg.kg-1, 1250 mg.kg-1, and TF at a dose of 200 mg .kg-1 feed. Feed and drinking water were given ad libitum. Meat sampling to measure SOD, catalase and MDA was taken at the end of the study by first chickens were fasted for ± 8 hours (overnight). The sample used was right breast meat of experimental male chicken. The data were average from three experimental chickens. The data obtained were analyzed for the variant with the general linear model on MINITAB (version 16), then tested the differences between treatments using Tukey's honestly significant difference (HSD). The results showed that the source of laurate had a significant effect on SOD, catalase, and MDA, while antioxidant concentration significantly decreased SOD and MDA compared with control. The interaction between lauric sources and antioxidant concentrations affected catalase and MDA. The use of coconut oil as much as 3% and antioxidant supplementation of Areca vestiaria Giseke in feed at a dose of 1250 mg kg-1 could decrease lipid oxidation product of meat to produce a healthy food product.
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Aksu, T., M. I. Aksu, M. A. Yoruk, and M. Karaoglu. 2011. Effects of organically-complexed minerals on meat quality. Br. Poult. Sci. 52: 558-563.
Amos. 2009. Gambir sebagai antibakteri dalam formulasi obat kumur. Jurnal Sains Teknologi Indonesia 11: 188-192.
Ariani, S. R. D., E. Susilowati, E. V. H. Susanti, and Setiyani. 2008. Uji aktivitas ekstrak methanol daun jambu biji (Psidium guajava L.) sebagai antifertilitas kontrasepsi pada tikus putih (Rattus norvegicus). Indo. J. Chem. 8: 264-270.
Dayrit, C. S. 2003. Coconut oil: Atherogenic or not? (What therefore causes atherosclerosis). Phil. J. Cardiol. 31: 97-104.
Delles, R. M., Y. L. Xiong, A. D. True, T. Ao, and K. A. Dawson. 2014. Dietary antioxidant supplementation enhance lipid and protein oxidative stability of chicken broiler meat trough promotion of antioxidant enzyme activity. Poult. Sci. 93: 1571-1570.
Duangyod, T., C. Palanuvej, and N. Ruangrungsi. 2014. (+)-Chatechin and (-)-epicatechin contens and antioxidant activity of commercial black catechu and pale catechu. JOCPR 6: 2225-2232.
Gonzales-Lamothe, R., G. Mitchell, M. Gattuso, M. S. Diarra, F. Malonin, and K. Bouarab. 2009. Plant antimicrobial agents and their effects on plant and human pathogens. Int. J. Mol. Sci. 10: 3400-3419.
Gopala-Krishna, A. G., G. Raj, A. S. Bhatnagar, P. K. Prasanth-Kumar, and P. Chandrashekar. 2010. Coconut oil: chemistry, production and its application-A Review. ICJ, Juli: 15-27.
Gu, X. H., S. S. Li, and H. Lin. 2008. Effects of hot environment and dietary protein level on growth performance and meat quality of broiler chickens. Asian-Aust. J. Anim. Sci. 21: 1616-1623.
Gunawijaya, F.A., R. Gandasentana, dan K. Wahyudi. 1999. Efek pemberian katekin the hijau pada pertumbuhan tumor kelenjar susu mencit strain GR. Jurnal Kedokteran Trisakti 18: 61-67.
Londok, J. J. M. R., W. Manalu, K. G. Wiryawan, and Sumiati. 2017. Growth performance, carcass characteristics and fatty acids profile of broilers supplemented with lauric acid and natural antioxidant from Areca vestiaria Giseke. Pak. J. Nutr. 16: 719-731.
Molineux, P. 2004. The use of stable free radical diphenyl picryl-hydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. Sci. Technol. 26: 211-219.
Nimse, S. B. and D. Pal. 2015. Free radicals, natural antioxidants, and their reaction mechanisms. RSC. Adv. 5: 27986-28006.
Pour-Reza, J. and M. A. Edriss. 1997. Effects of dietary sorghum of different tannin concentrations and tallow supplementation on the performance of broiler chicks. Br. Poult. Sci. 38: 512-517.
Preuss, H. G., B. Echard, M. Enig, I. Brook, and T. B. Elliot. 2005. Minimum inhibitory concentrations of herbal essential oils and monolaurin for gram-positive and gram-negative bacteria. Molecular and Cellular Biochemistry. 272: 29-34.
Salter, A. M. 2013. Impact of consumption of animal products on cardiovascular disease, diabetes, and cancer in developed countries. Animal Frontiers 3: 20-27.
Samosir, A. P., M. R. J. Runtuwene, and G. Citraningtyas. 2012. Uji aktivitas antioksidan dan total flavonoid pada ekstrak etanol pinang Yaki (Areca vestiaria). Pharmacon 1: 1-6.
Santi, M. A., Sumiati, and L. Abdulah. 2015. Cholesterol and malondialdehyde contents of broiler-chicken meat supplemented with Indigofera zolingeriana top leaf meal. Media Peternakan 38: 163-168.
Simbala, E., and T. Tallei. 2010. Ethnobotanical, proximate, and phytochemical studies of Areca vestiaria Giseke (Piang Yaki). International Conference on Medical Plants ICOMP Surabaya Juli 2010.
Siri-Torino, P. W., Q, Sun, F. B. Hu, and R. M. Krauss. 2010. Meta-analysis of prospective cohort studies evaluating the association of saturated fat with cardiovascular disease. Am. J. Clin. Nutr. 91: 535-546.
Skeaff, C. M. and J. Miller. 2009. Dietary fat and coronary heart disease: summary of evidence from prospective cohort and randomised controlled trials. Ann. Nutr. Metab. 55: 173-201.
Surai, P. F. 2003. Natural Antioxidants in Avian Nutrition and Reproduction. Nottingham University Press, Nottingham.
Valenzuela, A. B. and S. K. Nieto. 1996. Synthetic and natural antioxidant: food quality protectors. Grassas y Aceites 47: 186-196.
DOI: https://doi.org/10.21059/buletinpeternak.v42i2.31767
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