Effect of Dietary Rumen Undegraded Protein (RUP) Level on Nutrient Intake and Digestion of Lactating Dairy Cows


Andriyani Astuti(1), Rochijan Rochijan(2), Budi Prasetyo Widyobroto(3*)

(1) Department of Animal Nutrition and Feed Science, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
(2) Department of Animal Production, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
(3) Department of Animal Production, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
(*) Corresponding Author


The objective of this study was to examine the effect of different levels of rumen undegraded protein (RUP) in diets of lactating dairy cows on feed intake and nutrient digestion. The experiment was run according to completely randomized design with three treatments of complete feed rations containing different levels of rumen undegraded protein (RUP) and four replications for each treatment. In a digestibility trial, twelve Friesian Holstein cows were divided into three groups at each group consist of four cows and given three dietary treatments containing three levels of RUP, namely 7.43% of total crude protein /CP (P0), 8.49% of total CP (P1), and 9.45% of total CP (P2). Diets consisted of forages (King grass, Pennisetum hybrid) and concentrates at ratio of 50:50 in dry matter basis. The diets contained 18.25% CP and 61.75% total digestible nutrient (TDN). The cows fed diet (P0) were considered the control group. Drinking water was given ad libitum. This experiment was conducted for 21 days of adaptation period and seven days of collection period. In the collection period, samples of feed, refusal feed, and faecal were collected to get the chemical content includes dry matter (DM), organic matter (OM), ether extract (EE), crude fiber (CF), crude protein (CP), nitrogen-free extract (NFE) and total digestible nutrients (TDN). The data obtained were processed with one-way ANOVA (analysis of variance) and the difference existed between the means (P<0.05) would be analyzed with Duncan’s new multiple range test. The results showed that cows fed diet P2 (9.45% RUP in CP) in had significantly higher feed and nutrient intake (DM, OM, CP, CF, EE, TDN) and higher DM and OM digestibility compared to the other two treatments.


Dairy cows; Lactation; Nutrient digestion; Rumen undegraded protein

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Astuti, A., A. Agus and S.P.S. Budi, 2012. Pengaruh penggunaan high quality feed supplement terhadap konsumsi dan kecernaan nutrien sapi perah awal laktasi. Buletin Peternakan, 33: 81–87. DOI: 10.21059/buletinpeternak.v33i2.120

Bach, A., S. Calsamiglia and M.D. Stern. 2005. Nitrogen metabolism in the rumen. J. Dairy Sci., 88: E9-E21.

Bahrami-Yekdangi, H., M. Khorvash, G. R. Ghorbani, M. Alikhani, R. Jahanian and E. Kamalian. 2014. Effects of decreasing metabolizable protein and rumen-undegradable protein on milk production and composition and blood metabolites of Holssoytein dairy cows in early lactation. J. Dairy Sci. 97: 3707-3714.

Broderick, G. A. 2003. Effects of varying dietary protein and energy levels on the production of lactating dairy cows. J. Dairy Sci. 86: 1370-1381.

Bunnakit, K. and S. Khampa, 2011. Effect of rumen undegradable protein levels on performance of Thai native x Brahman beef cattle. J. Nutr. Pak. 10: 1163-1167. DOI: 10.3923/pjn.2011.1163.1167

Chumpawadee, S., K. Sommart, T. Vongpralub and V. Pattarajinda. 2006. Effects of synchronizing the rate of dietary energy and nitrogen release on ruminal fermentation, microbial protein synthesis, blood urea nitrogen and nutrient digestibility in beef cattle. Asian-Australas. J. Anim. Sci. 19: 181-188.

Decruyenaere, V., A. Buldgen and D. Stilmant, 2009. Factors affecting intake by grazing ruminants and related quantification methods: a review. Biotechnol. Agron. Soc. Environ. 13: 559-573.

Doepel, L., G. E. Lobley, J. F. Bernier, P. Dubreuil and H. Lapierre. 2009. Differences in splanchnic metabolism between late gestation and early lactation dairy cows. J. Dairy Sci. 92: 3233-3243. DOI: 10.3168/jds.2008-1595

Felisberto, N. R. O., M. T. Rodrigues, M. A. D. Bomfim, R. S. Matos and A. G. P. C. Cordeiro. 2011. Effects of different sources of protein on digestive characteristics, microbial, efficiency and nutrient flow in dairy goats. R. Bras. Zootec. 40: 2228-2234. DOI: 10.1590/S1516-35982011001000024

Hadgu, G. Z. 2016. Factors affecting feed intake and its regulation mechanisms in ruminants - A review. International Journal of Livestock Research, 6: 19-40. doi:10.5455/ijlr.20160328085909

Hanifa, A. 2008. Pengaruh pemberian ransum dengan kualitas berbeda terhadap profil darah, produksi susu dan pertambahan bobot badan sapi perah. Sains Peternakan 6: 26-33.

Hartadi, H., S. Reksohadiprodjo, dan A. D. Tillman. 2005. Tabel Komposisi Pakan Untuk Indonesia. Gadjah Mada University Press, Yogyakarta.

Huhtanen, P., E. Poutiainen and T. Mikkols. 1985. The Effect of Supplementation of Silage with rapeseed meal or Gasol-treated barley on the performance of growing cattle. J. Agric. Sci. Finland. 57: 57-84.

Hussein, H. S., R. M. Jordan and J. D. Stern. 1991. Ruminal protein metabolism and intestinal amino acid utilization as affected by dietary protein and carbohydrate sources in sheep. J. Anim. Sci. 69: 2134-2146. DOI: 10.2527/1991.6952134x

Kamalak, A., Ö. Canbolat, Y. Gürbüz and O. Özay, 2005. Protected protein and amino acids in ruminant nutrition. KSU. J. Sci. Eng. 8: 84-88.

Larsen, M. and N. B. Kristensen. 2009. Effect of abomasal glucose infusion on splanchnic amino acid metabolism in periparturient dairy cows. J. Dairy Sci. 92: 3306-3318. DOI: 10.3168/jds.2008-1889

Laudadio, V. and V. Tufarelli. 2010. Effects of pelleted total mixed rations with different rumen degradable protein on milk yield and composition of Jonica dairy goat. Small Rumin. Res. 90: 47-52.

McCarthy, J. R. D., T. H. Klusmeyer, J. L. Vicini, J. H. Clark and D. R. Nelson. 1989. Effects of source of protein and carbohydrate on ruminal fermentation and passage of nutrients to the small intestine of lactating cows. J. Dairy Sci. 72: 2002-2016. DOI: 10.3168/jds.S0022-0302(89)79324-3

McCormick, M. E., J. D. Ward, D. D. Redfearn, D. D. French, D. C. Blouin, A. M. Chapa and J. M. Fernandez. 2001. Supplemental dietary protein for grazing dairy cows: effect on pasture intake and lactation performance. J. Dairy Sci. 84: 896–907. DOI: 10.3168/jds.S0022-0302(01)74547-X

Morgan, D. J., R. F. Cody and P. K. Upton. 1989. Cereal digestion in the ruminant: 2. Effect of formaldehyde treatment of barley upon rumen degradability and nutrient flow to the intestine of sheep. Irish J. Agric. Res. 28: 43-48.

Nisa, M. U., A. Javaid, M. A. Shahzad and M. Sarwar. 2008. Influence of varying ruminally degradable to undegradable protein ratio on nutrient intake, milk yield, nitrogen balance, conception rate and days open in early lactating NiliRavi buffaloes (Bubalus bubalis). Asian-Australas. J. Anim. Sci. 21: 1303-1311.

NRC. 2001. Nutrient Requirements of Dairy Cattle. 7th edn. The National Academies Press. Washington DC.

Rochijan. 2014. Effect of supplementation rumen undegraded protein on production and reproduction dairy cows. Thesis, Universitas Gadjah Mada, Indonesia.

Schwab, C. G. 1995. Protected proteins and amino acids. Pages 115–141 in Biotechnology in Animal Feeds and Animal Feeding. R. J. Wallace and A. Chesson, ed. VCH Press, Weinheim, Germany.

Steel, R. G. D. and J. H. Torrie. 2003. Principles and Procedures of Statistics. 2 edn. Mc. Graw-Hill Book Co. Inc., New York.

Suwandyastuti, S. N. O. 2013. Pengaruh nisbah energi-protein, nitrogen-sulfur dan kalsium-fosfor terhadap produk metabolisme rumen dan kecernaan substrat. J. Agripet. 9: 9–13. DOI: 10.17969/agripet.v13i2.812

Widyobroto, B. P., Rochijan, Ismaya, Adiarto and Y. Y. Suranindyah. 2016. The impact of balanced energy and protein supplementation to milk production and quality in early lactating dairy cows. JITA. 41: 83-90.

DOI: https://doi.org/10.21059/buletinpeternak.v44i4.59155

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