PENGARUH INOKULUM CAMPURAN Lactobacillus plantarum DAN Saccharomyces cerevisiae TERHADAP KUALITAS ORGANOLEPTIK, FISIK, DAN KIMIA SILASE KULIT BUAH KAKAO

https://doi.org/10.21059/buletinpeternak.v39i1.6152

Muhammad Askari Zakariah(1*), Ristanto Utomo(2), Zaenal Bacruddin(3)

(1) 
(2) 
(3) 
(*) Corresponding Author

Abstract


This research was done to determine the effect of L. plantarum and S. cerevisiae innoculated into cocoa pod silage on organoleptic, physical and chemical quality. This experiment consist of four treatments namely silage of fresh harvested cocoa pods without inoculant as control (K); inoculated with L. plantarum (KLp); innoculated with S. cerevisiae (KSc); and innoculated with both L. plantarum and S. cerevisiae mixture (KLp+Sc) was cassava meal added as supplement. Each treatment was replicated 3 time. Ensilage was done for 21 days. Variables observed were properties of organoleptic (i.e. color, odor, texture and existence of fungal), physical (pH), and chemical (i.e. lactic acid content, content of dry matter (DM), organic matter (OM), crude protein (CP), ether extract (EE), crude fiber (CF) and nitrogen free extract (NFE)). Collected data of organoleptic characteristic were analyzed by non-parametric test (Hedonic KruskalWallis), whereas physical and chemical data analyzed by one-way analysis of variance and followed by Duncan’s New Multiple Range Test for significant difference. Result showed the inoculum have effect (P<0.05) on characteristic of organoleptic (color and avaibility fungus) and chemical (dry matter, organic matter, extract ether, and nitrogen free extract content). Silage with inoculation Lp+Sc on silage significantly (P<0.05) had higher DM and OM concentration (22.66% vs 20.38% and 94.43% vs 93.72%), higher NFE (75.46% vs 70.51%) and lower EE (0.15% vs 0.87%, respectively), compared to control. Based on result, it can be concluded that silage with L. plantarum and S. cerevisiae mixture have better quality.

(Key words: Cocoa pod, Lactobacillus plantarum, Saccharomyces cerevisiae, Silage)


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References

Adesoji, A. T., A. A. Ogunjobi, O. Ezekiel Fagade and O. Jacob Babayemi. 2010. Effect of Lactobacillus plantarum Starter Culture on the Microbial Succession, Chemical Composition, Aerobic Stability and Acceptability by

Ruminant of Fermented Panicum maximum Grass. AU J. T 14: 1-24.

Anggraeny, Y. N. and U. Umiyasih. 2009. Pengaruh fermentasi accharomyces cerevisiae terhadap kandungan nutrisi dan kecernaan ampas pati aren (Arenga pinnata). Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner.

Aquilina, G., G. A. Chesson, P. S. Cocconcelli, J. D. Knecht, N. A. Dierick, M. A. Gralak, J. Gropp, I. Halle, C. Hogstrand, R. Kroker, L. Leng, S. L.

Puente, A. L Haldorsen, A. Mantovani, G. Martelli, M. Mezes, D. Renshaw, M. Saarela, K. Sejrsen, and J. Westendorf. 2012. Scientific Opinion on the safety and efficacy of Lactobacillus plantarum (NCIMB 41028) and Lactobacillus plantarum (NCIMB 30148) as silage additives for all animal species EFSA Journal 10: 1-11.

Astuti, M. 1980. Rancangan Percobaan. Fakultas Peternakan, Universitas

Gadjah Mada, Yogyakarta.

Baker, S. B. and W. H. Summerson. 1941. The colorimetric determination of lactic acid in biological material J. Biol. Chem. 138: 546-554.

Barnes, R. F., C. J. Nelson, K. J. Moore and M. Collins. 2007. Forages The Science of Grassland Agriculture. Blackwell Publishing, Iowa.

Cai, S. and X. Dong. 2010. Cellulosilyticum ruminicola gen. nov., sp. nov., isolated from the rumen of yak, and reclassification of Clostridium lentocellum as Cellulosilyticum lentocellum comb. nov. Int. J. Sys. Evol. Microbiol. 60: 845-849.

Cherney, D. J. R., J. H. Cherney and L. E. Chase. 2004. Lactation performance Fries Holstein cows fed fescue, orchardgrass, or alfalfa silage. J. Dairy Sci. 87: 2268-2276.

Contreras-Govea, F. E., R. E. Muck, G. A. Broderick and P. J. Weimer. 2013. Lactobacillus plantarum effect on silage fermentation and in vitro microbial yield. Anim. Feed Sci. 179: 61-68.

Dalié, D. K. D., A. M. Deschamps and F. Richard-Forget. 2010. Lactic acid

bacteria–Potential for control of mould growth and mycotoxins: A review. Food Control 21: 370-380.

Despal, I. G. Permana, S. N. Safarina, dan A. J. Tatra. 2011. Penggunaan berbagai sumber karbohidrat terlarut air untuk meningkatkan kualitas silase daun rami. Media Peternakan 34: 69-76.

Heinritz, S. N., S. D. Martens, P. Avila and S. Hoedtke. 2012. The effect of inoculant and sucrose addition on the silage quality of tropical forage legumes with varying ensilability. Anim. Feed Sci. Tech. 174: 201-210.

Hippen, A. R., D. J. Schingoethe, K. F. Kalscheur, P. L. Linke, D. R. Rennich, M. M. Abdelqader and I. Yoon. 2010. Saccharomyces cerevisiae

fermentation product in dairy cow diets containing dried distillers grains plus solubles. J. Dairy Sci. 93: 2661-2669.

Isnandar. 2011. Silase isi rumen sebagai pakan pengganti hijauan jagung

terhadap produksi susu sapi perah peranakan Frisien Holstein. Disertasi

Fakultas Peternakan, Universitas Gadjah Mada, Yogyakarta. Jatkauskas, J. and V. Vrotniakiene. 2007.

Effect of Lactobacillus plantarum, Pediococcus acidilactici, Enterecoccus

faecium and Lactobacillus lactis microbial supplementation of grass

silage on the fermentation characteristic in rumen dairy cows. Vet.

Zootech 40: 29-34.

Johnson, H. E., R. J. Merry, D. R. Davies, D. B. Kell, M. K. Theodorou and G. W. Griffith. 2005. Vacuum packing: a model system for laboratory‐scale

silage fermentations. J. Appl. Microbiol. 981: 106-113.

Kamal, M. 1997. Kontrol Kualitas Pakan Ternak. Laboratorium Makanan

Ternak. Jurusan Nutrisi Makanan Ternak, Fakultas Peternakan, Universitas Gadjah Mada, Yogyakarta.

Kurnianingtyas, I. B., P. R. Pandasari, I. Astuti, S. D. Widyawati, dan W. P. S. Suprayogi. 2012. Pengaruh macam akselerator terhadap kualitas fisik,

kimiawi, dan biologi silase rumput kolonjono. Trop. Anim. Husb. 1: 7-14.

Lateef, A., J. Oloke, E. B. G. Kana, S. O. Oyeniyi, O. R. Onifade, A. O. Oyeleye, O. C. Oladosu and A. O. Oyelami. 2008. Improving the quality of agrowastes by solid-state fermentation: enhanced antioxidant activities and

nutritional qualities. World J. Microbiol. Biotechnol. 24: 2369-2374.

Li, Y. and N. Nishino. 2011. Monitoring the bacterial community of maize silage stored in a bunker silo inoculated with Enterococcus faecium, Lactobacillus plantarum and Lactobacillus buchneri. J. Appl. Microbiol. 110: 1561-1570.

Mastika, I. M., I. W. Supartha, A. W. Puger and I. K. M. Budiasa. 2011. Aspergilus niger significantly improved nutrient content and quality of cocoa waste (Theobroma cacao L.). The Excellence Research Universitas Udayana 34-38.

McDonald, P., R. A. Edwards and J. F. D.Greenhalgh. 2002. Animal Nutrition. Prentice Hall, London. Nahm, K. H. 1992. Practical Guide to Feed,

Forage and Water Analysis. Yoo Han Publisher, Seoul.

Nelson dan Suparjo. 2011. Penentuan lama fermentasi kulit buah kakao dengan Phanerochaeta crysosporium: evaluasi kualitas nutrisi secara kimiawi. Agrinak 1: 1-10.

Olubajo, F. O., M. M. Asonibare and E. O. Awolumate. 1987. Cocoa pod silage and cocoa pod grass silage in goat and sheep nutrition. Overcoming constraints to the efficient utilization of agricultural by-product as animal feed. Said A. N., B. H. Dzowela, A. A. Adegbola, J. A. Kategile, D. A. Little, M. A. Naga and S. Sibanda (eds). Proceeding of the Fourth Annual Workshop held at the Institute of Animal Research, Mankon station,

Bamenda, Cameroon.

Ranjit, N. K. and L. Kung. 2000. The effect of Lactobacillus buchneri, Lactobacillus plantarum, or a chemical preservative on the fermentation and aerobic stability of corn silage. J. Dairy Sci. 83: 526-535.

Ridla, M., N. Ramli, L. Abdullah and T. Toharmat. 2007. Milk yield quality and safety of dairy cattle fed silage composed of organic components of garbage. J. Ferment. Bioeng. 77: 572-574.

Sianipar, J. and K. Simanihuruk. 2009. Performans kambing sedang tumbuh yang mendapat pakan tambahan mengandung silase kulit buah kakao. Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner.

Sofyan, A., L. M. Yusiati, Y. Widyastuti and R. Utomo. 2011. Microbiological

characteristic and fermentability of king grass (Pennisetum hybrid) silage

treated by lactic acid bacteria-yeast inoculant consortium combined with

rice bran addtion. Journal of the Indonesian Tropical Animal Agriculture

: 265-272.

Soleh, A. Z. 2005. Ilmu Statistika Pendekatan Teoritis dan Aplikatif disertai Contoh Penggunaan SPSS. Penerbit Rakayasa Sains, Bandung.

Van Ranst, G., V. Fievez, J. De Reik and E. Van Bockstaele. 2009.

Influence of ensiling forages at different dry matters and silage additives on lipid metabolism and fatty acid composition. Anim. Feed Sci. Tech. 150: 62-74.

Zakariah, M. A. 2014. Pengaruh penggunaan inokulum campuran Lactobacillus plantarum dan Saccharomyces cereviriae pada silase kulit buah terhadap komposisi kimia dan kecernaan in vitro. Skripsi Fakultas

Peternakan, Universitas Gadjah Mada, Yogyakarta.



DOI: https://doi.org/10.21059/buletinpeternak.v39i1.6152

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