The Hematological and Biochemical Profiles of Wonosobo Sheep Blood in Various Physiological Conditions

Sarmin Sarmin Sarmin(1*), Pudji Astuti(2), Claude Mona Airin(3)

(1) Department of Physiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
(2) Department of Physiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
(3) Department of Physiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
(*) Corresponding Author


The study aimed at examining the hematological and biochemical profiles of Wonosobo sheep blood in various physiological conditions. There were 32 Wonosobo sheep in the various physiological conditions, including 17 ewe lamb of 1-2 months of age, 5 pregnant female sheep of 12-48 months of age at 3 months of pregnancy, 5 ewe of 12-48 months of age in second lactation period of 1-2 months and 5 ram of 12-48 months of age. Each of the sheep was put in individual sheepfold and given Pakchong-1 grass, dried kangkong, and concentrate. Drinking water was given ad libitum. Blood sample was drawn from jugular vein and put into tubes for blood hematological and biochemical analyses. The results of the study showed that total leukocyte and lymphocyte were high in young sheep, while neutrophils was high in pregnant female sheep (P<0.05). There was not any significant physiological impact on hemoglobin, MCV, MCH, MCHC, RDW, hematocrit, platelet, monocyte, eusinophil, and basophil (P>0.05). The highest ALP activity and Pi levels were found in the ewe lamb, while low creatinine was found in the ram (P<0.05). The parameter of the activities of SGPT, SGOT, LDH, calcium, kalium, chloride, sodium, magnesium, TIBC, UIBC, TS, globulin, albumin, total protein, BUN, creatinine, glucose, cholesterol, triglyceride, HDL and LDL was not affected by physiological conditions (P>0.05). It was concluded that the impact of the physiological conditions of the Wonosobo sheep caused high total leukocyte and lymphocyte in ewe lamb and high neutrophil in pregnant female sheep, the increase in the Pi level and ALP activity of the ewe lamb, and high creatinine in the ram


Ewe lamb; Hematology; Physiological conditions; Pregnant; Wonosobo sheep

Full Text:



Abdel-Fattah, M. S., A. L. S. Hashem, Y. M. Shaker, A. M. Ellamei, and H. Z. Amer. 2013. Effect of weaning age on productive performance and some plasma biochemical parameters of Barki lambs in Siwa Oasis, Egypt. Glob. Vet. 10: 189–202.

Ahmadi-hamedani, M., K. Ghazvinian, N. Atyabi, P. Khanalizadeh, M. A. Masoum, and M. S. Ghodrati. 2016. Hematological reference values of healthy adult Sangsari sheep (Iranian fat-tailed sheep) estimated by Reference Value Advisor. Comp. Clin. Path. 25: 459–464.

Al-Bulushi, S., T. Shawaf, and A. Al-Hasani. 2017. Some hematological and biochemical parameters of different goat breeds in Sultanate of Oman “A preliminary study.” Vet. World 10: 461–466.

Alonso, A. J., R. Teresa, M. García, J. R. González, and M. Vallejo. 1997. The Effects of age and reproductive status on serum and blood parameters in Merino breed sheep. J. Vet. Med. A 44: 223–231.

Antunovic, Z., J. Novoselec, H. Sauerwein, M Speranda, and M. Vegara. 2011. Blood metabolic profile and some of hormones concentration in ewes during different physiological status. Bulgarian Journal of Agricultural Science 17: 687–695.

Antunović, Z., M. Šperanda, Đ. Senčić, J. Novoselec, Z. Steiner, and M. Djidara. 2012. Influence of age on some blood parameters of lambs in organic production. Maced. J. Anim. Sci. 1: 11–15.

Antunović, Z., M. Šperanda, and Z. Steiner.. 2004. The influence of age and the reproductive status to the blood indicators of the ewes. Arch. Anim. Breed. 47: 265–273.

Ashour, G., Neama, A. Ashmawy, S. M. Dessouki, and O. H. Shihab. 2015. Blood hematology, metabolites and hormones in newborn sheep and goat from birth to weaning. Int. J. Adv. Res. 3: 1377–1386.

Azimzadeh, K., and and A. Javadi. 2020. Serum biochemistry and haematology of Iranian Red Sheep (Ovis orientalis gmelini) in Sorkhabad Protected Area, Zanjan, Iran: Comparison with age and sex. Iran. J. Vet. Med. 14: 76–84.

Badawi, N. and H. AL-Hadithy. 2014. The Hematological parameters in clinically healthy Iraqi Awassi sheep. World’s Vet. J. 6: 01.

Barsila, S. R., K. Bhatt, B. Devkota, and N. R. Devkota. 2020. Haematological changes in transhumant Baruwal sheep (Ovis aries) grazing in the western Himalayan mountains in Nepal. Pastoralism 10: 1–10.

Bhat, S., M. Mir, A. Reshi, S. Ahmad, I. Husain, S. Bashir, and M. Khan. 2014. Impact of age and gender on some blood biochemical parameters of apparently healthy small ruminants of sheep and goats in Kashmir valley India. International Journal of Agricultural Sciences and Veterinary Medicine 2: 22–27.

Borjesson, D. L., M. M. Christopher, and W. M. Boyce. 2000. Biochemical and hematologic reference intervals for free-ranging desert bighorn sheep. J. Wildl. Dis. 36: 294–300.

Bórnez, R., M. B. Linares. and H. Vergara. 2009. Haematological, hormonal and biochemical blood parameters in lamb: Effect of age and blood sampling time. Livest. Sci. 121: 200–206.

Buddle, B. M., G. Jowett, R. S. Green, P. G. C. Douch, and P. L. Risdon.1992. Association of blood eosinophilia with the expression of resistance in Romney lambs to nematodes. Int. J. Parasitol. 22: 955–960.

Cavender, C. P., S. D. Turley, and J. M. Dietschy. 1995. Sterol metabolism in fetal, newborn, and suckled lambs and their response to cholesterol after weaning. Am. J. Physiol. Endocrinol. Metab. 269: E331–E340.

Carlson, G. P. 1997. Fluid, electrolyte, and acidbase balance. In: Clinical biochemistry of domestic animals. Kaneko, J. J. Harvey, J. W. Bruss, M. L. (Eds). 5th edn. San Diego: Academic. pp. 485-516.

Chaney, S. G. 1997. Principles of nutrition II: Micronutrients. In: Textbook of biochemistry with clinical correlations. 4 edn. Devlin, T. M. (Ed). Wiley Liss, Inc., New York, p.1107-1136.

Cihan, H., E. M. Temizel, Z. Yilmaz, and Y. Ozarda. 2016. Koyunlarda doğum öncesi ve sonrası serum demir durumu ve hematolojik endekslerle İlişkisi. Kafkas Universitesi Veteriner Fakultesi Dergisi.

Cruz, R. E. S. da, F. M. Rocha, C. V. B. Sena, P. G.Noleto, E. C. Guimarães, J. A. Galo, and A. V. Mundim. 2017. Effects of age and sex on blood biochemistry of dorper lambs. Semina: Ciências Agrárias, 38: 3085–3093.

Daramola, J., A. Adeloye, T. Fatoba, and A. Soladoye. 2005. Hematological and biochemical parameters of West African Dwarf goat. Livest. Res. Rural Dev. 17: 1–8.

de Souza, D. F., T. S. S. S. Reijers, S. Gilaverte, T. A. da Cruz, F. Hentz, B. de Q. Castilhos, R. L. Dittrich, and A. L. G. Monteiro. 2020. Dynamics of biochemical parameters in lambs during the first four months of life. Rev. Bras. de Zootec. 49: e20190167.

Demir, A. Ö., K. Irak, H. Mert, N. Mert, N. Ayşin. and I. Sogutlu. 2020. Honamli goats breed in south of Turkey I- serum mineral analysis. Turkish Journal Agriculture Food Science and Technology 8: 1910–1917.

Dias, I. R., C. A. Viegas, A. M. Silva, H. F., Pereira, C. P. Sousa, P. P., Carvalho, A. S. Cabrita, P. J. Fontes, S. R. Silva, and J. M. T. Azevedo. 2010. Haematological and biochemical parameters in Churra-da-Terra-Quente ewes from the northeast of Portugal. Arq. Bras. Med. Vet. Zootec. 62: 265–272.

Elnageeb, M. E. and A. M. Abdelatif. 2013. Growth, thermoregulation and hematological responses of lambs in relation to age and maternal nutritional supplementation. J. Biological Sci. 13: 323–331.

Fava, C., F. Cattazzo, Z.-D. Hu, G. Lippi, and M. Montagnana. 2019. The role of red blood cell distribution width (RDW) in cardiovascular risk assessment: Useful or hype?. Ann. Transl. Med. 7: 581–581.

Feldman, B. F., J. G. Zink, and N. C. Jain. 2002. Schalm’s Veterinary Hematology. Lippincott Williams and Wilkins. Philadelphia, USA.

Fernandes, S. R., A. L. G. Monteiro, R. L. Dittrich, J. A. Salgado, C. J. A. da Silva, M. G. B. da Silva, O. C. Beltrame, and P. H. N. Pinto. 2012. Early weaning and concentrate supplementation on the performance and metabolic profile of grazing lambs. Rev. Bras. de Zootec. 41: 1292–1300.

Habibu, B., A. Abdullahi, L. Yaqub, H. Makun, M. Kawu, and S. Ahmadu. 2017. Variations in platelet count and total protein in relation to differences in sex,age, breed and reproductive status of goats during the cold-dry season (Harmattan). J. Dairy Vet. Anim. Res. 5:

Hakim, F. R., M. Arifin, and E. Rianto. 2019. Growth pattern and productivity of female Wonosobo sheep in Wonosobo District, Central Java Province, Indonesia. IOP Conference Series: Earth and Environmental Science 247: 012-044.

Jackson, P. and P. Cockcroft. 2002. Clinical Examination of Farm Animals. Blackwell Publishing. Osney Mead, Oxford, UK.

Jain, N. 1986. In: Schalm’s Veterinary Hematology. 4th edn. Jain N. C. ed. Lea and Febiger. Philadelphia, USA.

Kaneko, J. J., J. W. Harvey, and M. L. Bruss. 2008. Clinical Biochemistry of Domestic Animals. 6th edn. Academic Press, Inc. New York, USA.

Karthik, D., J. Suresh, Y. R. Reddy, G. R. K. Sharma, J. V. Ramana, G. Gangaraju, P. P. R. Reddy, Y. P. K. Reddy, D. Yasaswini, M. J. Adegbeye, and P. R. K. Reddy. 2021. Adaptive profiles of Nellore sheep with reference to farming system and season: Physiological, hemato-biochemical, hormonal, oxidative-enzymatic and reproductive standpoint. Heliyon: e07117.

Kock, M. D., Clark, R. K. Franti, C. E. Jessup, D. A. and J. D Wehausen. 1987. Effects of capture on biological parameters in free-ranging bighorn sheep (Ovis Canadensis): evaluation of normal, stressed and mortality outcomes and documentation of postcapture survival. J. Wildl. Dis. 23: 652–662.

Kozat, S., N. Yüksek, Y. Göz, and I. Keleş. 2006. Serum iron, total iron-binding capacity, unbound iron-binding capacity, transferrin saturation, serum copper, and hematological parameters in pregnant Akkaraman ewes infected with gastro-intestinal parasites. Turk. J. Vet. Anim. Sci. 30: 601–604.

Kramer, J. W. 2000. Normal Hematology of Cattle, Sheep, and Goats In: Schalm’s Veterinary Hematology. Kramer, B. F., J. G, Zinkl, N. C. Jain (Eds). 5th edn. pp. 1057–1084. Lippincot Williams & Wilkins. Baltimore, USA.

Lepherd, M., P Canfield, Hunt, G. and K. Bosward. 2009. Haematological, biochemical and selected acute phase protein reference intervals for weaned female Merino lambs. Aust. Vet. J. 87: 5–11.

May, J. E., M. B. Marques, V. V. B. Reddy, and R. Gangaraju. 2019. Three neglected numbers in the CBC: The RDW, MPV, and NRBC count. Clevel. Clin. J. Med. 86: 167–172.

Mazzullo, G., C. Rifici, G. Caccamo, Rizzo, M. and G. Piccione. 2014. Effect of different environmental conditions on some haematological parameters in cow. Annals Anim. Sci. 14: 947–954.

Mohammed, A., A. Khan, I. Pargass, P. Bridgemohan, A. E. Edwards, H. S. Stewart, F. G. Youssef, and S. Sieuchand, 2017. Serum mineral levels in goats of various physiological stages in the dry and wet seasons in central trinid. Микроэлементы в Медицине 18: 18–27.

Mostaghni, K., K. Badiei, and M. Emadi 2005. Haematology and serum biochemistry of captive wild sheep (Ovis orientalis esphahanica) in Iran. Comp. Clin. Path. 13: 158–161.

Murphy, W. G. 2014. The sex difference in haemoglobin levels in adults-Mechanisms, causes, and consequences. Blood Rev. 28: 41–47.

Nagy, O., C. Tóthová, and G. Kováč. 2014. Age-related changes in the concentrations of serum proteins in calves. J. Appl. Anim. Res. 42: 451–458.

Okonkwo, J., I. Okonkwo, and G. Ebuh. 2011. Effect of breed, sex and source within breed on the haematological parameters of the nigerian goats. J. Anim. Feed Res. 1: 8–13.

Pernthaner, A., Cole, S. A. Morrison, L. and W. R. Hein. 2005. Increased Expression of Interleukin-5 (IL-5), IL-13, and tumor necrosis factor alpha genes in intestinal lymph cells of sheep selected for enhanced resistance to nematodes during Infection with Trichostrongylus colubriformis. Infect. Immun. 73: 2175–2183.

Piccione, G., Caola, G. Giannetto, C. Grasso, F., Runzo, S. C. Zumbo, A. and P. Pennis 2009. Selected biochemical serum parameters in ewes during pregnancy, post-parturition, lactation and dry period. Anim. Sci. Pap. Reports 27: 321-220

Pizetti, A. J. M., R. O. Sarmiento, L. A. Pintos, G. B. Trova, J. A. Binda, and O. S. Negrette. 2021. Haematological and protein profile of goat rodeo in extensive productions of different regions in the province of Salta, Argentina. J. Appl. Anim. Res. 49: 239–246.

Polizopoulou, Z. S. 2010. Haematological tests in sheep health management. Small Rumin. Res. 92: 88–91.

Pošiváková, T., J. Švajlenka, J. Pošivák, J. Pokorádi, R. Hromada, P. Korim, and L. Molnár. 2019. The influence of age on the activity of selected biochemical parameters of the mouflon (Ovis musimon L.). Animals 9: 242.

Rahman, Md. K., S. Islam, J. Ferdous, Md. H. Uddin, M. B. Hossain, M. M.Hassan, and A. Islam. 2018. Determination of hematological and serum biochemical reference values for indigenous sheep (Ovies aries) in Dhaka and Chittagong Districts of Bangladesh. Vet. World 11: 1089–1093.

Ramos, J. J., M. T. Verde, M. C. Marca, and A. Fernández. 1994. Clinical chemical values and variations in Rasa Aragonesa ewes and lambs. Small Rumin. Res. 13: 133–139.

Ribeiro, N. L., R. G. Costa,. E. C. P. Filho, M. N. Ribeiro, and R. Bozzi. 2018. Effects of the dry and the rainy season on endocrine and physiologic profiles of goats in the Brazilian semi-arid region. Ital. J. Anim. Sci. 17: 454–461.

Russell, K. E. and A. J. Roussel.2007. Evaluation of the ruminant serum chemistry profile. Vet. Clin. North Am. Food Anim. Pract. 23: 403–426.

Salvagno, G. L., F. Sanchis-Gomar, A. Picanza, and G. Lippi. 2015. Red blood cell distribution width: A simple parameter with multiple clinical applications. Crit. Rev. Clin. Lab. Sci. 52: 86–105.

Sarmin, S. Winarsih, A., Hana, P. Astuti, and C. M. Airin. 2021a. Parameters of blood biochemistry in different physiological status of fat-tailed sheep. AIP Conference Proceedings 2353, 030070.

Sarmin, S. Winarsih, A., Hana, P. Astuti, and C. M. Airin. 2021b. Haematological profiles of Indonesian fat-tailed sheep under different physiological conditions. Trop. Anim. Health Prod. 53: 523.

Soliman, E. B. 2014. Effect of physiological status on some hematological and biochemical parameters of ossimi sheep. Egyptian Journal of Sheep & Goat Sciences 9: 33–42.

Suswono. 2011. Keputusan Menteri Pertanian Nomor 2915/Kpts/OT.140/6/2011 tentang Penetapan Rumpun Domba Wonosobo.

Tibbo, M., K. Aragaw, F. Abunna, M. Woldemeskel, A. Deressa, M. L. Dechassa, and J. E. O. Rege. 2005. Factors affecting haematological profiles in three indigenous Ethiopian sheep breeds. Comp. Clin. Path. 13: 119–127.

Weiss, D. and K. Wardrop 2010. Schalms Veterinary Haematology 6th edn. Wiley- Blackwell, Ammes, Iowa USA.

Žura Žaja, I., Vince, S. N. P. Milas, I. R. A. Lobpreis, B. Špoljarić, A. S. Vugrovečki, S. Milinković-Tur, M. Šimpraga, L. Pajurin, T. Mikuš, K. Vlahović, M. Popović, and D. Špoljarić. 2019. A new method of assessing sheep red blood cell types from their morphology. Animals 9: 1130.


Article Metrics

Abstract views : 1237 | views : 693


  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Buletin Peternakan (Bulletin of Animal Science) Indexed by:

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.