Gelatinase Microbial Morphology from Leather Defect
Novita Kurniawati(1), Yuny Erwanto(2), Zaenal Bachrudin(3), Ambar Pertiwiningrum(4*)
(1) Departemen of Animal Product Technology, Faculty of Animal Scince, Universitas Gadjah Mada, Yogyakarta, 55281,
(2) Departemen of Animal Product Technology, Faculty of Animal Scince, Universitas Gadjah Mada, Yogyakarta, 55281,
(3) Departement of Animal Nutrition and Feed Science, Faculty of Animal Scince, Universitas Gadjah Mada, Yogyakarta, 55281,
(4) Departemen of Animal Product Technology, Faculty of Animal Scince, Universitas Gadjah Mada, Yogyakarta, 55281,
(*) Corresponding Author
Abstract
Microbes attach in pickle leather, wet blue leather, and upper shoeleather have been identified to have positive profile to produce extracellular protease enzymes with pH values of acidic to basic on 15% gelatin and 2% commercial skimmed milk as substrates. Defective tanned leather was added to a microbial growth solution enriched with 2% gelatin substrate and incubated for 7 days at room temperature on an open rotary shaker at 120 rpm and then stored at room temperature while waiting for gelatinase testing. There were 5 morphological colonies of microbial gelatinase from defective tanned skin, all of which were white.
Keywords
Full Text:
PDFReferences
Cadirci, B. H., H. Ozgunay, C. Vural, and O. Yilmaz. 2010. A new defect on leather: Microbial bio-film. Journal of the American Leather Chemists Association 105: 129- 133. https://journals.uc.edu/index.php/ JALCA/article/view/3255/2506
Gautam, M. and W. Azmi. 2017. Screening and Isolation of Collagenase Producing Microorganism from Proteins Waste Found in Himalayan Region. J. Appl. Biotechnology Reports 4: 558–565. http://www.biotechrep.ir/article_68978.html
Gioia, M., G. F. Fasciglione, S. Monaco, R. Iundusi, D. Sbardella, S. Marini, U. Tarantino, and M. Coletta. 2010. pH dependence of the enzymatic processing of collagen I by MMP-1 (fibroblast collagenase), MMP-2 (gelatinase A), and MMP-14 ectodomain. JBIC 15: 1219–1232. https://doi.org/10.1007/s00775-010-0680-8
Hermawan, P. 2014. Teknologi Pengolahan Kulit. Akademi Teknologi Kulit Yogyakarta, Indonesia.
Juwon, A. D. and O. F. Emmanuel. 2012. Experimental investigations on the effects of carbon and nitrogen sources on concomitant amylase and polygalacturonase production by Trichoderma viride BITRS-1001 in submerged fermentation. Biotechnology Research International 2012: 1–8. https://doi.org/10.1155/2012/904763
Kohn, J. 1953. A Preliminary report of a new gelatin liquefaction method. Journal of Clinical Pathology 6: 249–249. https://doi.org/10.1136/jcp.6.3.249
Kurniawati, N., E. Lusiana, N. A. Fitriyanto, M. Z. Abidin, S. Rakhmatulloh, Y. Erwanto, Z. Bachruddin, Rusman, L. M. Yusiati, and A. Pertiwiningrum. 2021a. Future benefits of microorganism on leather defects in the industrial production of protease. BIO Web of Conferences 33: 1-13. https://doi.org/10.1051/bioconf/202133020 02
Kurniawati, N., V. Pastawan, R. Yuliatmo, Y. Erwanto, and A. Pertiwiningrum. 2022b. The effect of storage time to the leather microstructure due to collagenolytic bacteria activity. BT - Proceedings of the 9 th International Seminar on Tropical Animal Production (ISTAP 2021). pp.182– 184. https://doi.org/10.2991/absr.k. 220207.038
Kusmaryanti, T., R. Ibrahim, and P. H. Riyadi. 2016. Pengaruh perbedaan bahan penyamak terhadap kualitas kulit ikan pari mondol (Himantura gerrardi) tersamak. Indonesian Journal of Fisheries Science and Technology 11: 140. https://doi.org/10. 14710/ijfst.11.2.140-147
Negi, S. and R. Banerjee. 2010. Optimization of culture parameters to enhance production of amylase and protease from Aspergillus awamori in a single fermentation. Afr. J. Biochem Res. 4: 73-80.
Oruko, R. O., O. J. Odiyo, and N. J. Edokpayi. 2019. The role of leather microbes in human health: In Role of Microbes in Human Health and Diseases. IntechOpen. 2019: 1-17 https://doi.org/10.5772/ intechopen.81125
Orlita, A. 2004. Microbial biodeterioration of leather and its control: a review. International Biodeterioration and Biodegradation 53: 157–163. https://doi. org/10.1016/S0964-8305(03)00089-1
Purnomo, E. 2016. Teknologi Penyamakan Mineral. Akademi Teknologi Kulit Yogyakarta, Indonesia.
Rachmawati, L., T. Novianti, and M. S. Wiyanti. 2018. Perbandingan kualitas kulit sapi Wet Blue dengan masa penyimpanan berbeda. Berkala Penelitian Teknologi Kulit, Sepatu, dan Produk Kulit 77: 21–31.
DOI: https://doi.org/10.21059/buletinpeternak.v46i4.77044
Article Metrics
Abstract views : 811 | views : 530Refbacks
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Buletin Peternakan (Bulletin of Animal Science) Indexed by:
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.