Pengaruh Suplementasi Probiotik Bakteri Asam Laktat Terhadap Histomorfologi Usus Dan Performan Puyuh Jantan

Sapta Chandra Marnadi Hidayat(1*), Sri Harimurti(2), Lies Mira yusiati(3)

(*) Corresponding Author


The present study was conducted to investigate the effects of lactic acid bacteria (LAB) probiotics supplementation on intestinal histomorpholgy and performance of male quail. The LAB probiotics supplement contained of three strains, those are Lactobacillus murinus, Streptococcus thermophilus, and Pediococcus acidilactici. A total of 96 day-old  male quails (DOQ) were randomly divided into four groups of LAB probiotic supplementation reared for 42 days. Oral supplementation of LAB probiotic were 0 (P0), 107(P1), 108 (P2), and 109(P3)CFU/mL/bird/day. Data were analyzed by analysis of variance CRD One-Way Anova continued with Duncan’s New Multiple Range Test (DMRT). The results showed that LAB probiotic treatment affected on the intestinal morphology, body weight gain, and feed conversion of male quail (P <0.05). Height of intestinal villi were 288.45±8.18, 322.72±1.84, 324.82±2.06, and 390.80±13.24 (µm) respectively. Width of intestinal villi were 112.82±2.35, 138.62±2.35, 162.57±17.15 and 130.72±13.45 (µm) respectively. Depth of intestinal crypts were 61.25±1.49, 72.50±6.25, 69.85±4.55, and 69.05±4.57 (µm) respectively. Feed intake were not different, but resulted difference body weight gain, which were 102.85±4.72, 109.20±4.13, 115.07±7.61, and 118.75±2.54 (g), respectively. Feed conversion were 4.30±0.43, 4.26±0.17, 3.91±0.30, and 3.79±0.27, respectively. This study indicated that supplementation of 109 CFU/mL/bird/day of LAB probiotics showed the best result.


Histomorphology; Male quail; Performance; Probiotics LAB


Abdel-Raheem, S. M., M. S. S. Abd-Allah , and M. A. K. Hassanein. 2012. The Effects of Prebiotic, Probiotic and Synbiotic Supplementation on Intestinal Microbial Ecology and Histomorphology of Broiler Chickens. IJAVMS 6 (4): 277-289.

Ahrens, K. E. S., P. Ade, B. Marten, P. Weber, W. Timm, Y. Asil, C. C. Gluer, and J. Schrezenmeir. 2007. Prebiotics, Probiotics, and Synbiotics Affect Mineral Absorption, Bone Mineral Content, and Bone Structure. J. Nutr. 137: 838S–846S.

Antoine, J. M. 2010. Probiotics in the defence and metabolic balance of the organism Probiotics: beneficial factors of the defence system. The 3rd International Immunonutrition Workshop. Proceedings of the Nutr. Soc. 69: 429-433.

Arslan, C. and M. Saattci. 2004. Effect of Probiotics admininstation either as feed additive or by drinking water on performance and blood parameters of japanesse quail. Arch. Geflugelk. 68: 160 – 163.

Besten, G. D., K. V. Eunen , A. K. Groen, K. Venema, D. J. Reijngoud, and B. M. Bakker. 2013. The role of short-chain fatty acids in the interplay between diet, gut microbiota, and host energy metabolism. J. Lipid Res. (54): 2325–2340.

Castanon, J. I. R. 2007. History of the use of antibiotic as growth promoters in European poultry feeds. J. Poult. Sci. 86: 2466-2471

Donoghue, D. J., 2003. Antibiotic residues in poultry tissues and eggs: Human health concerns. J. Poult. Sci. 82: 618–621.

Fioramonti, J., V. Theodorou, and L. Bueno. 2003. Probiotics: what are they? What are their effects on gut physiology?. Best Practice & Research Clinical Gastroenterology Vol. 17 No. 5 pp. 711–724.

Gómez, S., M. L. Angeles, M. C. Mojica, and S. Jalukar. 2012. Combination of an Enzymatically Hydrolyzed Yeast and Yeast Culture with a Direct-fed Microbal in the feeds of Broiler Chickens. Asian-Aust. J. Anim. Sci. 25 (5) : 665-673.

Ghosh, H. K., G. Halder, G. Samanta, S. K. Paul, and S. K. Pyne. 2007. Effect of Dietary Supplementation of organic Acid and Mannan Oligosaccharide on the Performance and Gut Health of Japanese Quail (Cortunix cortunix japonica). Asian J. Poult. Sci. 1 (1): 1-7.

Harimurti, S., J. P. H. Sidadolog, Wihandoyo, T. Yuwanta, S. Sudaryati, H. Sasongko, dan B. Ariyadi. 2014. Dinamika Probiotik Indigenous Bakteri Asam Laktat Pada Kinerja Pertumbuhan, Produksi Asam Lemak Rantai Pendek, Dan Total Bakteri Aderensi Dalam Usus Puyuh Jantan. Laporan Penelitian Hibah Penelitian Tematik Laboratorium. Fakultas Peternakan UGM. Yogyakarta.

Harimurti, S. and Widodo. 2014. Effect of indigenous probiotics lactic acid bacteria on performance, intestinal length and weight of internal organs of broiler chicken. ICOBM 2014 D-8.

Herich, R. and M. Levkut. 2002. Lactic acid bacteria, probiotics and immune system. Vet. Med. Czech. 47 (6): 169-180.

Ichikawa, H., T. Kuroiwa, A. Inagaki, R. Shineha, T. Nishihira, S. Satomi, and T. Sakata. 1999. Probiotic bacteria stimulate gut epithelial cell proliferation in rat. Dig. Dis. Sci. 44 (10): 2119-2123.

Jeppsson, B., P. Mongell, D. Adawi, and G. Molin. 2004. Bacterial translocation: impact of probiotics. Scandinavian J. Nutr. 48 (1): 37-41.

Jin, L. Z., Y. W. Ho, N. Abdullah, and S. Jalaludin. 2000. Digestive and bacterial enzyme activities in broilers fed diets supplemented with lactobacillus cultures. Poult. Sci. 79:886-891.

Kabir, S. M. L. 2009. The role of probiotics in the poultry industry. Int. J. Mol. Sci. 10: 3531-3546.

Mroz, Z. 2005. Organic Acids as Potential Alternatives to Antibiotic Growth Promoters for Pigs. Advances in Pork Production 16:169.

Ohashi, Y. and K. Ushida. 2009. Health-beneficial effects of probiotics: Its mode of action. J. Anim. Sci. 80: 361-371.

Pelicano, E. R. L., P. A. Souza, H. B. A. Souza, D. F. Figueiredo, M. M. Boiago, S. R. Carvalho, and V. F. Bordon. 2005. Intestinal Mucosa Development in Broiler Chickens Fed Natural Growth Promoters. Braz. J. Poult. Sci. 7 (4): 221- 229.

Perić, L., N. Milošević, D. Zikić, S. Bjedov, D. Cvetković, S. Markov, M. Mohnl and T. Steiner. 2010. Effects of probiotic and phytogenic products on performance, gut morphology and cecal microflora of broiler chickens. Archiv. Tierzucht 53(3): 350-359.

Pym, R. A. E. 2005. Genetic aspects of food intake and food utilisation efficiency for growth in chickens. Aust. Poult. Sci. Symp. (17):153–62.

Resta, S. C. 2009. Effects of Probiotics and Commensals on Intestinal Epithelial Physiology: Implications for Nutrient Handling. J. Physiol. 587 (17): 4169-4174.

Roto, S. M., P. M. Rubinelli and S.C. Ricke. 2015. An introduction to the avian gut microbiota and the effects of yeast-based prebiotic-type compounds as potential feed additives. Front. Vet. Sci. 2:28.

Scott, T. A. 2005. Variation in feed intake of broiler chickens. Recent Advances in Animal Nutrition in Australia 15:237-244

Vali, N. 2009. Probiotic in quail nutrition: a review. Int. J. Mol. Sci. 8 (12): 1218-1222.

Van Immerseel, J. Russel, M. Flythe, I. Gantois, L. Timbermont, F. Pasmans, F. Haesebrouck, and R. Ductalle. 2006. The use organic acids to combat salmonella in poultry: a mechanistic explanation of the efficacy. Avian pathol. 35 (03): 182-188.


Article Metrics

Abstract views : 2566 | views : 4728


  • There are currently no refbacks.

License URL:

Buletin Peternakan (Bulletin of Animal Science) Indexed by:

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.