Agus Hadi Prayitno(1*)

(*) Corresponding Author


The experiment was conducted to analysis the effect of nanoparticle eggshell calcium lactate fortification on chemical and physical properties of chicken meatballs. The materials were chicken meat, filler, spices, salt, eggshell calcium and nanoparticle calcium lactate. Fortification level of eggshell calcium and nanoparticle calcium lactate on making meatballs was 0.3% of the total meatball dough. Each treatment consisted of five replications. The data of chemical and physical properties of meatballs were analyzed statistically using variance analysis. The differences between means were tested by Duncan's new Multiple Ranges Test. The meatball fortified with eggshell calcium showed lower moisture content 67.92%, and showed higher fat content 6.92% and ash 2.56% than those of nanoparticle eggshell calcium lactate. The meatballs fortified with eggshell calcium had higher pH value 7.34 and water-holding capacity 58.53% and more firmness than those of fortified with eggshell calcium lactate.

(Keywords: Chemical and physical properties, Chicken meatballs, Eggshell, Fortification, Nanoparticle calcium lactate)

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Adak, M. D. and K. M. Purohit. 2011. Synthesis of nano-crystalline hydroxyapatite from dead snail shells for biological implantation. Trends Biomater. Artif. Organs. 25: 101-106.

Alvarado, C. and S. McKee. 2007. Marination to improve functional properties and safety of poultry meat. J. Appl. Poult. Res. 16: 113-120.

Amu, O. O., A. B. Fajobi and B. O. Oke. 2005. Effect of eggshell powder on the stabilizing potential on lime on an expansive clay soil. J. Applied Sci. 5: 1474-1478.

AOAC. 2005. Official Methods of Analysis of the Association of Analytical Chemist. 18th edn. Association of Official Analytical Chemist, Washington DC.

Bouton, P. E., P. V. Harris and W. R. Shorthose. 1971. Effect of ultimate pH upon the water-holding capacity and tenderness of mutton. J. Food Sci. 36: 435-439.

Bouton, P. E., P. V. Harris and W. R. Shorthose. 1972. The effect of ultimate pH on ovine muscle: water holding capacity. J. Food. Sci. 37: 351-355.

Boyle, E. A. E., P. B. Addis and R. J. Epley. 1994. Calcium fortified, reduced fat beef emulsion product. J. Food Sci. 59: 928-932.

Buckle, K. A., R. A. Edwards, G. H. Fleet and M. Wooton. 1985. Ilmu Pangan. Diterjemahkan oleh Purnomo, H dan Adiono. Cetakan Ke-1. UI Press, Jakarta.

Candogan, K. and N. Kolsarıcı. 2003. The effects of carrageenan and pectin on some quality characteristics of low – fat beef frankfurters. Meat Sci. 64: 199-203.

Chemaly, Z., H. Muhr and M. Fick. 1999. Crystallization kinetics of calcium lactate in a mixed-suspension-mixed-product removal crystallizer. Ind. Eng. Chem. Res. 38: 2803-2808.

Code of Federal Regulations, Food and Drug Administration. 1997. Calcium lactate; Title 21, 184.1207. US Government Printing Office, Washington, DC.

Daengprok, W., W. Garnjanagoonchorna and Y. Mine. 2002. Fermented pork sausage fortified with commercial or hen eggshell calcium lactate. Meat Sci. 62: 199-204.

Daengprok, W., W. Garnjanagoonchorn, O. Naivikul, P. Pornsinlpatip, K Issigonis, and Y. Mine. 2003. Chicken eggshell matrix proteins enhance calcium transport in the human intestinal epithelial cells, Caco-2. J. Agric. Food Chem. 51: 6056-6061.

Direktorat Jenderal Peternakan dan Kesehatan Hewan. 2013. Statistik Peternakan dan Kesheatan Hewan 2013. Direktorat Jenderal Peternakan dan Kesehatan Hewan Kementerian Pertanian RI, Jakarta.

Edwards, H. M. Jr. 1981. Carcass composition studies. 3. Influence of age, sex and calorie protein contents of the diet on carcass composition of Japanese quail. J. Poult. Sci. 60: 2506-2512.

Gongruttananun, H. 2011. Effects of eggshell calcium on productive performance, plasma calcium, bone mineralization, and gonadal characteristics in laying hens. Poult. Sci. 90: 524-529.

Hadi, Wisnu. S. 2005. Sifat fisik dan organoleptik minuman instan madu bubuk dengan penambahan efek effervescent dari tepung kerabang telur. Skripsi, Institut Pertanian Bogor, Bogor.

Hamm, R. 1986. Functional properties of the myofibrillar system and their measurement. In: Muscle as Food. Bechtel, P. J. (ed). Academic Press, New York. pp. 135-199.

Hanzlik, R. P., S. C. Fowler and D. H. Fisher. 2005. Relative bioavailability of calcium from calcium formate, calcium citrate, and calcium carbonate. J. Pharmacol. Exp. Ther. 313: 1217-1222.

Hervera, M., C. Castrillo, E. Albanell and M. D. Baucells. 2012. Use of near-infrared spectroscopy to predict energy content of commercial dog food. J. Anim. Sci. 90: 4401-4407.

Hunton, P. 2005. Research on eggshell structure and quality: An historical overview. Braz. J. Poult. Sci. 7: 67-71.

Lawrie, R. A. 2003. Ilmu Daging. Edisi Ke-5. Penerjemah: Parakkasi, A. Universitas Indonesia Press, Jakarta.

Lestari, S. 1991. Bisnis Bakso yang Menguntungkan. Media Pertanian DKI, Jakarta.

Li, Z., Y. Zhang and T. Tan. 2009. Preparation of edible nano calcium lactate crystal from crude L-lactic acid via chemical precipitation method. J. Biosci. Bioeng. 108: S138.

Malsch, N. H. 2005. Biomedical Nanotechnology. CRC Press, Boca Raton.

Naruki, S. dan S. Kanoni. 1992. Kimia dan Teknologi Pengolahan Hasil Hewani. Pusat Antar Universitas Pangan dan Gizi, Universitas Gadjah Mada, Yogyakarta.

Niewoehner, C. 1988. Calcium and osteoporosis. Cereal Foods World, 33: 784-787.

Nurlela, A., S.U. Dewi, K. Dahlan, dan D.S. Soejoko. 2013. Pemanfaatan Limbah Cangkang Telur Ayam dan Bebek sebagai Sumber Kalsium untuk Sintesis Mineral Tulang. Jurnal Pendidikan Fisika Indonesia 10: 81-85.

Ockerman, H. W. and C. L. Hansen. 2000. Animal By-Product Processing and Utilization. CRC Press, Boca Raton.

Omi, N. and I. Ezawa. 1998. Effect of eggshell Ca on preventing of bone loss after ovariectomy. J. Home Econ. Jpn. 49: 277-282.

Pearson, A. M. and R. B. Young. 1989. Meat and Biochemistry. Academy Press Inc., California.

Phil, G. and M. Zhihong, 2009. High value products from hatchery waste. RIRDC publication no. 09/061. preparations.

Pigott, R. S., P. B. Kenney, S. Slider and M. K. Head. 2000. Formulation protocol and dicationic salts affect protein functionality of model system beef batters. J. Food Sci. 65: 1151-1154.

Poulain, N. and E. Nakache. 1998. Nanoparticles from vesicles polymerization. II. Evaluation of their encapsulation capacity. J. Polym. Sci. 36: 3035-3043.

Purnomo, H. and D. Rahardiyan. 2008. Review Article: Indonesian traditional meatball. Int. Food Res. J. 15: 101-108.

Rohman, A., Sismindari, Y. Erwanto and Y. B. C. Man. 2011. Analysis of pork adulteration in beef meatball using Fourier transform infrared (FTIR) spectroscopy. Meat Sci. 88: 91-95.

Schaafsma, A. and I. Pakan. 1999. Short-term effects of a chicken egg shell powder enriched dairy-based products on bone mineral density in persons with osteoporosis or osteopenia. Bratisl. Lek. Listy. 100: 651-656.

Schaafsma, A. and G. M. Beelen. 1999. Eggshell powder, a comparable or better source of calcium than purified calcium carbonate: piglet studies. J. Sci. Food Agric. 79: 1596-1600.

Schaafsma, A., I. Pakan, G. J. H. Hofstede, F. A. J. Muskiet, E. Van Der Veer and P. J. F. De Vries. 2000. Mineral, amino acid, and hormonal composition of chicken eggshell powder and the evaluation of its use in human nutrition. Poult. Sci. 79: 1833-1838.

Setiyono. 2008. Rekstrukturisasi Daging Sapi untuk Pangan Kesehatan : Studi pada Ratus Norvegicus L. Disertasi. Program Studi Ilmu dan Industri Peternakan, Sekolah Pascasarjana Universitas Gadjah Mada, Yogyakarta.

Shelef, L. A. and V. Potluri. 1995. Behaviour of foodborne pathogens in cooked liver sausage containing lactates. Food Microbiol. 12: 221-227.

Soeparno. 2009. Ilmu dan Teknologi Daging. Cetakan Ke-5. Gadjah Mada Press, Yogyakarta.

Stadelman, W. J. 2000. Eggs and Egg Products: Encyclopedia of Food Science and Technology John Wiley & Sons, New York. pp. 593-599.

Standar Nasional Indonesia. 1995. Bakso Daging Sapi. (SNI 01-3818-1995). Badan Standardisasi Nasional, Jakarta.

Steel, R. G. D. dan J. H. Torrie. 1993. Prinsip dan Prosedur Statistik. Suatu Pendekatan Biometrik. Diterjemahkan oleh M. Syah. PT Gramedia Pustaka Utama, Jakarta.

Tsugawa, N., T. Okano, R. Higashino, T. Kimura, Y. Oshio, Y. Teraoka, C. Igarashi, I. Ezawa and T. Kobayashi. 1995. Bioavailability of calcium carbonate, DL-calcium lactate, L-calcium lactate, and powdered oyster shell calcium in vitamin D deficient or -replete rats. Biol. Pharm. Bull. 18: 677-682.

Walton, H. V., O. J. Cotterill and J. M. Vandepopuliere. 1973. Composition of shell waste from egg breaking plants. Poult. Sci. 52: 1836-1841.

Wang, L., I. Sondi and E. Matijević. 1999. Preparation of uniform needle-like aragonite particles by homogeneous precipitation. J. Colloid Interface Sci. 218: 545-553.

Wang, Y., L. Huang and J. Wu. 2012. Optimization of conditions for calcium lactate nano-particle production. Adv. Materials Res. 479: 314-317.

Weaver, C. 1998. Calcium in food fortification strategies. Int. Dairy J. 8: 443-449.


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