Evaluation of Kleinhovia hospita Metabolite Profiles Exposed to Diverse Gamma-Irradiation Doses by 1H-NMR Combined with Chemometric Modeling

https://doi.org/10.22146/ijc.103509

Islamudin Ahmad(1), Lizma Febrina(2*), Herman Herman(3), Baso Didik Hikmawan(4), Amal Rezka Putra(5), Junaidin Junaidin(6), Arman Rusman(7), Arsyik Ibrahim(8), Hifdzur Rashif Rija'i(9), Erwin Samsul(10), Muhammad Arifuddin(11), Supriatno Salam(12), Iswahyudi Iswahyudi(13), Gemini Alam(14), Abdul Mun'im(15)

(1) Pharmaceutical Research and Development Laboratory of FARMAKA TROPIS, Faculty of Pharmacy, Universitas Mulawarman, Kampus Gunung Kelua, Samarinda 75119, Indonesia; Department of Research and Development, PT. Borneo Riseta Naturafarm, Jl. Simpur No. 47, Tenggarong Sebrang, Kutai Kertanegara 75572, Indonesia
(2) Pharmaceutical Research and Development Laboratory of FARMAKA TROPIS, Faculty of Pharmacy, Universitas Mulawarman, Kampus Gunung Kelua, Samarinda 75119, Indonesia; Department of Research and Development, PT. Borneo Riseta Naturafarm, Jl. Simpur No. 47, Tenggarong Sebrang, Kutai Kertanegara 75572, Indonesia
(3) Pharmaceutical Research and Development Laboratory of FARMAKA TROPIS, Faculty of Pharmacy, Universitas Mulawarman, Kampus Gunung Kelua, Samarinda 75119, Indonesia; Department of Research and Development, PT. Borneo Riseta Naturafarm, Jl. Simpur No. 47, Tenggarong Sebrang, Kutai Kertanegara 75572, Indonesia
(4) Pharmaceutical Research and Development Laboratory of FARMAKA TROPIS, Faculty of Pharmacy, Universitas Mulawarman, Kampus Gunung Kelua, Samarinda 75119, Indonesia; Department of Research and Development, PT. Borneo Riseta Naturafarm, Jl. Simpur No. 47, Tenggarong Sebrang, Kutai Kertanegara 75572, Indonesia
(5) Research Center for Radioisotope, Radiopharmaceutical, and Biodosimetry Technology, Research Organization for Nuclear Energy, National Research and Innovation Agency (BRIN), Kawasan Puspiptek, Building 720, Serpong, Banten 15314, Indonesia
(6) Pharmaceutical Research and Development Laboratory of FARMAKA TROPIS, Faculty of Pharmacy, Universitas Mulawarman, Kampus Gunung Kelua, Samarinda 75119, Indonesia; Department of Research and Development, PT. Borneo Riseta Naturafarm, Jl. Simpur No. 47, Tenggarong Sebrang, Kutai Kertanegara 75572, Indonesia
(7) Pharmaceutical Research and Development Laboratory of FARMAKA TROPIS, Faculty of Pharmacy, Universitas Mulawarman, Kampus Gunung Kelua, Samarinda 75119, Indonesia; Department of Research and Development, PT. Borneo Riseta Naturafarm, Jl. Simpur No. 47, Tenggarong Sebrang, Kutai Kertanegara 75572, Indonesia
(8) Pharmaceutical Research and Development Laboratory of FARMAKA TROPIS, Faculty of Pharmacy, Universitas Mulawarman, Kampus Gunung Kelua, Samarinda 75119, Indonesia; Department of Research and Development, PT. Borneo Riseta Naturafarm, Jl. Simpur No. 47, Tenggarong Sebrang, Kutai Kertanegara 75572, Indonesia
(9) Pharmaceutical Research and Development Laboratory of FARMAKA TROPIS, Faculty of Pharmacy, Universitas Mulawarman, Kampus Gunung Kelua, Samarinda 75119, Indonesia; Department of Research and Development, PT. Borneo Riseta Naturafarm, Jl. Simpur No. 47, Tenggarong Sebrang, Kutai Kertanegara 75572, Indonesia
(10) Pharmaceutical Research and Development Laboratory of FARMAKA TROPIS, Faculty of Pharmacy, Universitas Mulawarman, Kampus Gunung Kelua, Samarinda 75119, Indonesia; Department of Research and Development, PT. Borneo Riseta Naturafarm, Jl. Simpur No. 47, Tenggarong Sebrang, Kutai Kertanegara 75572, Indonesia
(11) Pharmaceutical Research and Development Laboratory of FARMAKA TROPIS, Faculty of Pharmacy, Universitas Mulawarman, Kampus Gunung Kelua, Samarinda 75119, Indonesia; Department of Research and Development, PT. Borneo Riseta Naturafarm, Jl. Simpur No. 47, Tenggarong Sebrang, Kutai Kertanegara 75572, Indonesia
(12) Pharmaceutical Research and Development Laboratory of FARMAKA TROPIS, Faculty of Pharmacy, Universitas Mulawarman, Kampus Gunung Kelua, Samarinda 75119, Indonesia; Department of Research and Development, PT. Borneo Riseta Naturafarm, Jl. Simpur No. 47, Tenggarong Sebrang, Kutai Kertanegara 75572, Indonesia
(13) Department of Research and Development, PT. Borneo Riseta Naturafarm, Jl. Simpur No. 47, Tenggarong Sebrang, Kutai Kertanegara 75572, Indonesia
(14) Department of Pharmacognosy, Faculty of Pharmacy, Hasanuddin University, Jl. Perintis Kemerdekaan Km. 10 Kampus UNHAS Tamalanrea, Makassar, 90245, Indonesia
(15) Department of Pharmacognosy-Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Jl. Kukusan Beji, Depok 16424, Indonesia
(*) Corresponding Author

Abstract


Gamma irradiation is a widely used method for decontaminating medicinal plants, including Kleinhovia hospita (Tahongai). However, the high energy inherent in this process, coupled with the paucity of scientific reports detailing changes in the K. hospita metabolite profile, necessitates an investigation into its effects. Hence, this study examined the effects of gamma irradiation at varying doses (0–25 kGy) on the metabolite profile of the ethanolic extract of K. hospita utilizing 1H-NMR combined with a chemometric approach. The concentrations of some identified metabolites present in the sample were determined by the quantitative 1H-NMR technique. Our investigation successfully identified 20 metabolites in the K. hospita sample. Both the quantitative analysis and the PLS-DA modeling demonstrated that gamma irradiation doses up to 5 kGy induced no significant differences in the metabolite profiles (p-value > 0.05), indicating high similarity with the control group. Conversely, a significant alteration in the metabolite profile emerged only at an exposure dose of 10 kGy or higher, characterized by the loss of characteristic amino acid and cycloartane triterpenoid signals. Consequently, the 10 kGy level signifies the point at which substantial alterations to the K. hospita metabolome are initiated, thereby demanding prudence in its application.

Keywords


1H-NMR; metabolomic; Tahongai; gamma-irradiation; PLSDA



References

[1] Damayanti, S., Fabelle, N.R., Yooin, W., Insanu, M., Jiranusornkul, S., and Wongrattanakamon, P., 2021, Molecular modeling for potential cathepsin L inhibitor identification as new anti-photoaging agents from tropical medicinal plants, J. Bioenerg. Biomembr., 53 (3), 259–274

[2] Frankova, A., Vistejnova, L., Merinas-Amo, T., Leheckova, Z., Doskocil, I., Wong Soon, J., Kudera, T., Laupua, F., Alonso-Moraga, A., and Kokoska, L., 2021, In vitro antibacterial activity of extracts from Samoan medicinal plants and their effect on proliferation and migration of human fibroblasts, J. Ethnopharmacol., 264, 113220.

[3] Ariefta, N.R., Sofian, F.F., Aboshi, T., Kuncoro, H., Dinata, D.I., Shiono, Y., and Nishikaw, Y., 2024, Evaluation of the antiplasmodial and anti-Toxoplasma activities of several Indonesian medicinal plant extracts, J. Ethnopharmacol., 331, 118269

[4] Rahim, A., Saito, Y., Miyake, K., Goto, M., Chen, C.H., Alam, G., Morris-Natschke, S., Lee, K.H., and Nakagawa-Goto, K., 2018, Kleinhospitine E and cycloartane triterpenoids from Kleinhovia hospita, J. Nat. Prod., 81 (7), 1619–1627.

[5] Alani, F.W., Djabir, Y.Y., and Arsyad, M.A., 2023, Kleinhovia hospita leaf extract protects the heart against infarction by isoproterenol, Iran. J. Toxicol., 17 (2), 87–94.

[6] Tayeb, R., Alam, G., Pakki, E., and Djabir, Y.Y., 2019, Paliasa (Kleinhovia hospita L.) hepatoprotector ‘tea bag’ preparation as supporting therapy in the use of fixed-dose combination of antituberculosis drugs, J. Phys.: Conf. Ser., 1341 (7), 072016.

[7] Opuni, K.F.M., Kretchy, J.P., Agyabeng, K., Boadu, J.A., Adanu, T., Ankamah, S., Appiah, A., Amoah, G.B., Baidoo, M., and Kretchy, I.A., 2023, Contamination of herbal medicinal products in low-and-middle-income countries: A systematic review, Heliyon, 9 (9), e19370.

[8] Casu, A., Camardo Leggieri, M., Toscano, P., and Battilani, P., 2024, Changing climate, shifting mycotoxins: A comprehensive review of climate change impact on mycotoxin contamination, Compr. Rev. Food Sci. Food Saf., 23 (2), e13323.

[9] Molnár, K., Rácz, C., Dövényi-Nagy, T., Bakó, K., Pusztahelyi, T., Kovács, S., Adácsi, C., Pócsi, I., and Dobos, A., 2023, The effect of environmental factors on mould counts and AFB1 toxin production by Aspergillus flavus in maize, Toxins, 15 (3), 227.

[10] Ansari, F.A., Perazzolli, M., Husain, F.M., Khan, A.S., Ahmed, N.Z., and Meena, R.P., 2024, Novel decontamination approaches for stability and shelf-life improvement of herbal drugs: A concise review, Microbe, 3, 100070.

[11] Rahmati, E., Khoshtaghaza, M., Banakar, A., and Ebadi, M.T., 2022, Decontamination technologies for medicinal and aromatic plants: A review, Food Sci. Nutr., 10 (3), 784–799.

[12] Schottroff, F., Lasarus, T., Stupak, M., Hajslova, J., Fauster, T., and Jäger, H., 2021, Decontamination of herbs and spices by gamma irradiation and low-energy electron beam treatments and influence on product characteristics upon storage, J. Radiat. Res. Appl. Sci., 14 (1), 380–395.

[13] Hadiati, S.W., Winarno, H., and Pramono, S., 2021, Gamma irradiation as suitable preservation method on herbal medicine, Food Res., 5 (5), 33–42.

[14] Riviello-Flores, M.L., Cadena-Iñiguez, J., Ruiz-Posadas, L.D., Arévalo-Galarza, M.D., Castillo-Juárez, I., Soto Hernández, M., and Castillo-Martínez, C.R., 2022, Use of gamma radiation for the genetic improvement of underutilized plant varieties, Plants, 11 (9), 1161.

[15] Komara, I., Susanto, A., Amaliya, A., Abbas, B., Warastuti, Y., Hendiani, I., Mirada, A., and Erliani, A.P., 2023, The effect of gamma-ray irradiation on the physical, mechanical, and morphological characteristics of PVA-collagen-chitosan as a guided tissue regeneration (GTR) membrane material, Eur. J. Dent., 17 (2), 530–538.

[16] Aleksieva, K., Mladenova, R., Taneva, S., Denev, P., and Karakirova, Y., 2025, Effect of gamma irradiation on free radicals and the antioxidant properties of walnuts, Chemistry, 7 (2), 52.

[17] Aleksieva, K.I., Mladenova, R.B., Solakov, N.Y., Loginovska, K.K., and Dimov, K.G., 2025, Gamma radiation effects on free radicals, antioxidant activity, phenolic and flavonoid content in royal jelly, Radiat. Phys. Chem., 226, 112231.

[18] Galeaz, C., Totis, C., and Bisio, A., 2021, Radiation resistance: A matter of transcription factors, Front. Oncol., 11, 662840.

[19] Katiyar, P., Pandey, N., and Keshavkant, S., 2022, Gamma radiation: A potential tool for abiotic stress mitigation and management of agroecosystem, Plant Stress, 5, 100089.

[20] Choi, H.I., Han, S.M., Jo, Y.D., Hong, M.J., Kim, S.H., and Kim, J.B., 2021, Effects of acute and chronic gamma irradiation on the cell biology and physiology of rice plants, Plants, 10 (3), 439.

[21] Ahmad, I., Febrina, L., Hikmawan, B.D., Junaidin, J., Herman, H., Faisal, M., Samsul, E., Iswahyudi, I., Ibrahim, A., Purwoko, R.Y., Okselni, T., Angelina, M., Putra, A.R., Alwi, R.S., and Mun’im, A., 2024, NMR-based metabolic profiling and antioxidant activity of Eleutherine bulbosa bulb ethanol extract, Nat. Prod. Res., 39 (24), 7096–7103.

[22] Netrprachit, P., Nuangchamnong, N., Ramli, N.S., Maulidiani, M., Mediani, A., Zolkeflee, N.K.Z., Jiamyangyuen, S., and Abas, F., 2023, Bioactive compounds of pigmented rice extracts reveal by NMR-based metabolomics, CyTA – J. Food, 21 (1), 760–770.

[23] Febrina, L., Happyana, N., and Syah, Y.M., 2023, Metabolic profiling, antioxidant activity, and alpha-glucosidase inhibitory activity of the roasted beans of luwak (civet) coffee, ACS Food Sci. Technol., 3 (11), 1864–1876.

[24] Satmoko, A., Gunawan, H.A., Kardos, R., Rozali, B., and Purwadi, M.D, 2020, Commissioning test of the iradiator gamma merah putih, J. Phys.: Conf. Ser., 1436 (1), 012017.

[25] Gao, X., Niu, J., Xiao, Z., Rong, D., Yu, M., Sy, B., Wang, C., and Lv, Z., 2025, The application of quantitative 1H-NMR for the determination of melatonin and vitamin B6 in commercial melatonin products, Molecules, 30 (14), 2942.

[26] Dona A.C., Kyriakides, M., Scott, F., Shephard, E.A., Varshavi, D., Veselkov, K., and Everett, J.R., 2016, A guide to the identification of metabolites in NMR-based metabonomics/metabolomics experiments, Comput. Struct. Biotechnol. J., 14, 135–153.

[27] Alexandersson, E., and Nestor, G., 2022, Complete 1H and 13C spectral assignment of D-glucofuranose, Carbohydr. Res., 511, 108477.

[28] Febrina, L., Happyana, N., and Syah, Y.M., 2022, Identifying metabolites in complex extract of sigararutang coffee beans with NMR spectroscopy method, Moroccan J. Chem., 10 (2), 298–307.

[29] Devi, K., and Awasthi, P., 2022, Isoleucine with secondary sulfonamide functionality as anticancer, antibacterial and antifungal agents, J. Biomol. Struct. Dyn., 40 (15), 7052–7069.

[30] Chaloupecká, E., Tyrpekl, V., Bártová, K., Nishiyama, Y., and Dračínský, M., 2024, NMR crystallography of amino acids, Solid State Nucl. Magn. Reson., 130, 101921.

[31] Asmarani, R.R., Ujilestari, T., Solikin, M.M., Wulandari, W., Damayanti, E., Anwar, M., Aditya, S., Karimy, M.F., Wahono, S.K., Triyannanto, E., Adli, D.N., Surjawanta, S.O., and Wahyono, T., 2024, Meta-analysis of the effects of gamma irradiation on chicken meat and meat product quality, Vet World, 17 (5), 1084–1097.

[32] Hota, S., Shams, R., Dash, K.K., Pawase, P.A., Mukarram, S.A., and Kovács, B., 2025, A comprehensive review on the potential role of irradiation technique on techno-functional properties of plant-based proteins, J. Agric. Food Res., 23, 102095.

[33] da Silva, M.M., Diniz, E.C., Maia Neto, L.S., Araujo, L.A., Magnata, S.S.L.P., de Jesus Amaral, A., Maciel Netto, A., and da Silva, E.B., 2025, Analysis of the relationship between absorbed dose and its effect on plant materials: A literature review, Braz. J. Radiat. Sci, 13 (3A), 1–24.

[34] Li, X., Wang, B., Hu, W., Chen, H., Sheng, Z., Yang, B., and Yu, L., 2022, Effect of γ-irradiation on structure, physicochemical property and bioactivity of soluble dietary fiber in navel orange peel, Food Chem.: X, 14, 100274.

[35] Majumdar, C.G., ElSohly, M.A., Ibrahim, E.A., Elhendawy, M.A., Stanford, D., Chandra, S., Wanas, A.S., and Radwan, M.M., 2023, Effect of gamma irradiation on cannabinoid, terpene, and moisture content of cannabis biomass, Molecules, 28 (23), 7710.



DOI: https://doi.org/10.22146/ijc.103509

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