Antihypercholesterolemic Activity of Ethanol Extract from  Manilkara kauki  Leaves:  In Vitro  and  In Silico  Studies

Renita Meilasari; Tukiran Tukiran

doi:10.22146/ijc.110256

Antihypercholesterolemic Activity of Ethanol Extract from Manilkara kauki Leaves: In Vitro and In Silico Studies

https://doi.org/10.22146/ijc.110256

Renita Meilasari⁽¹⁾, Tukiran Tukiran^(2*)

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Surabaya, Jl. Ketintang, Surabaya 60231, Indonesia
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Surabaya, Jl. Ketintang, Surabaya 60231, Indonesia
(*) Corresponding Author

Abstract

Current food consumption patterns are a significant concern in the field of health. For example, the habit of consuming high-calorie, high-saturated-fat, and high-sugar junk food contributes to various health problems. Fat, as a molecule required by the body as a source of energy, has adverse effects if its levels exceed the amount needed by the body. One of these effects is an increase in cholesterol levels, a condition known as hypercholesterolemia. Manilkara kauki is a plant that has the potential as an antihypercholesterolemic agent. The objective of this study is to evaluate their antihypercholesterolemic activity both in vitro, using the Liebermann-Burchard method, and in silico, by assessing the inhibition of the HMG-CoA reductase enzyme (PDB ID: 1HW9), which plays a key role in cholesterol synthesis. The antihypercholesterolemic activity assessed via the Liebermann-Burchard assay revealed that the ethanol extract exhibited significant activity, with an IC₅₀ value of 108.91 ppm. Furthermore, LC-MS analysis identified 117 compounds in the extract, which were subjected to molecular docking studies. Three flavonoid compounds, lanaroflavone (−8.69 kcal/mol), sequoiaflavone (−9.28 kcal/mol), and sumaflavone (−8.55 kcal/mol), demonstrated higher binding affinities to HMG-CoA reductase than the positive control, simvastatin (−8.53 kcal/mol).

Keywords

Manilkara kauki; antihypercholesterolemic; Liebermann-Burchard; HMG-CoA reductase

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DOI: https://doi.org/10.22146/ijc.110256