The Cytotoxicity of Phenolic Sesquiterpenes from  Dysoxylum parasiticum  Leaves Against MCF-7 Human Breast Cancer Cells

Ferry Ferdiansyah Sofian; Amrina Virliana; Ahda Salsabila Izzaturrahmi; Ellin Febrina; Eri Bachtiar; Tjandrawati Mozef; Aiyi Asnawi; Kindi Farabi; Nurlelasari Nurlelasari; Yasmiwar Susilawati; Anas Subarnas; Unang Supratman; Yoshihito Shiono

doi:10.22146/ijc.110617

The Cytotoxicity of Phenolic Sesquiterpenes from Dysoxylum parasiticum Leaves Against MCF-7 Human Breast Cancer Cells

https://doi.org/10.22146/ijc.110617

Ferry Ferdiansyah Sofian^(1*), Amrina Virliana⁽²⁾, Ahda Salsabila Izzaturrahmi⁽³⁾, Ellin Febrina⁽⁴⁾, Eri Bachtiar⁽⁵⁾, Tjandrawati Mozef⁽⁶⁾, Aiyi Asnawi⁽⁷⁾, Kindi Farabi⁽⁸⁾, Nurlelasari Nurlelasari⁽⁹⁾, Yasmiwar Susilawati⁽¹⁰⁾, Anas Subarnas⁽¹¹⁾, Unang Supratman⁽¹²⁾, Yoshihito Shiono⁽¹³⁾

(1) Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia; Study Center of Herb, Faculty of Pharmacy, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia; Central Laboratory of Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia
(2) Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia
(3) Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia
(4) Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia
(5) Department of Marine Science, Faculty of Fishery and Marine Science, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia
(6) Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency (BRIN), Jl. Raya Bogor Km. 46, Cibinong 16911, Indonesia
(7) Department of Pharmacochemistry, Faculty of Pharmacy, Universitas Bhakti Kencana, Jl. Soekarno-Hatta No. 754, Bandung 40617, Indonesia
(8) Central Laboratory of Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia
(9) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia
(10) Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia; Study Center of Herb, Faculty of Pharmacy, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia
(11) Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia
(12) Central Laboratory of Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia
(13) Department of Food, Life, and Environmental Science, Faculty of Agriculture, Yamagata University, 1-23 Wakaba-machi, Tsuruoka City, Yamagata 997-8555, Japan
(*) Corresponding Author

Abstract

The sesquiterpene phenols from Dysoxylum parasiticum leaves have received limited study, particularly regarding their mechanisms of action against breast cancer and their associated molecular targets. Thus, this study isolated bioactive secondary metabolites from D. parasiticum leaves and evaluated their cytotoxicity and molecular interactions with breast cancer targets. Two previously characterized sesquiterpene phenol derivatives, dysoxyphenol (1) and 7-hydroxycalamenene (2), were isolated from D. parasiticum leaf extracts. The chemical characterization was established through comprehensive spectral analyses and confirmed by optical rotation data. Cytotoxic evaluation demonstrated notable bioactivity against MCF-7 cells, with half-maximal inhibitory concentrations of 196.02 and 94.44 μM for compounds 1 and 2, respectively. Computationally, molecular docking and molecular dynamics simulations revealed that both compounds 1 and 2 exhibit stable interactions with a breast cancer-human estrogen receptor (PDB ID: 3ERT). The results suggest that these findings contribute to understanding the anticancer potential of sesquiterpene phenol derivatives from D. parasiticum, making them promising candidates for developing novel cancer drugs.

Keywords

sesquiterpene phenols; Dysoxylum parasiticum; Meliaceae; cytotoxicity; MCF-7 cells

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DOI: https://doi.org/10.22146/ijc.110617