Comparative Analysis of Dexamethasone Adulteration in Herbal Anti-Rheumatic Products Using HPLC, UV-Vis Spectrophotometer, and FTIR Coupled with Chemometrics

Anggita Rosiana Putri; Ni Made Ajeng Gayatri Maitri Paramitha; Bachtiar Rifai Pratita Ihsan; Mazlina binti Mohd Said

doi:10.22146/ijc.111442

Comparative Analysis of Dexamethasone Adulteration in Herbal Anti-Rheumatic Products Using HPLC, UV-Vis Spectrophotometer, and FTIR Coupled with Chemometrics

https://doi.org/10.22146/ijc.111442

Anggita Rosiana Putri^(1*), Ni Made Ajeng Gayatri Maitri Paramitha⁽²⁾, Bachtiar Rifai Pratita Ihsan⁽³⁾, Mazlina binti Mohd Said⁽⁴⁾

(1) Department of Pharmacy, Faculty of Medicine, Universitas Brawijaya, Jl. Veteran, Malang 65145, Indonesia; Drug Development and Analytical Methods Research Group, Faculty of Medicine, Universitas Brawijaya, Jl. Veteran, Malang 65145, Indonesia
(2) Department of Pharmacy, Faculty of Medicine, Universitas Brawijaya, Jl. Veteran, Malang 65145, Indonesia
(3) Department of Pharmacy, Faculty of Medicine, Universitas Brawijaya, Jl. Veteran, Malang 65145, Indonesia; Drug Development and Analytical Methods Research Group, Faculty of Medicine, Universitas Brawijaya, Jl. Veteran, Malang 65145, Indonesia
(4) Center of Drug and Herbal Research, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, Malaysia
(*) Corresponding Author

Abstract

Herbal anti-rheumatic products are widely consumed in Indonesia. Despite regulations prohibiting the use of active pharmaceutical ingredients (APIs) in traditional medicines, cases of adulteration are still reported nowadays. This study aimed to evaluate the applicability of high-performance liquid chromatography (HPLC), UV-vis spectrophotometry, and Fourier transform infrared (FTIR) spectroscopy combined with chemometrics analysis for the detection of dexamethasone in herbal anti-rheumatic products from Malang City. All analytical methods were validated and met the required performance criteria. The results showed that none of the analyzed herbal products contained dexamethasone. These findings demonstrate that the developed analytical approaches are reliable for detecting adulteration in herbal preparations and may serve as effective screening tools for routine quality control of herbal products.

Keywords

dexamethasone; herbal products; HPLC; UV-vis spectrophotometry; FTIR; chemometrics

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DOI: https://doi.org/10.22146/ijc.111442