Validation of UV-Vis Spectrophotometric Method for Catechin Determination in Kawangkoan Peanut Skin Extract Nanoemulsion as an Antioxidant

Irma Antasionasti; Surya Sumantri Abdullah; Utami Sasmita Lestari

doi:10.22146/ijc.115349

Validation of UV-Vis Spectrophotometric Method for Catechin Determination in Kawangkoan Peanut Skin Extract Nanoemulsion as an Antioxidant

https://doi.org/10.22146/ijc.115349

Irma Antasionasti^(1*), Surya Sumantri Abdullah⁽²⁾, Utami Sasmita Lestari⁽³⁾

(1) Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Sam Ratulangi, Jl. Kampus Unsrat Kleak, Manado 95115, Indonesia
(2) Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Sam Ratulangi, Jl. Kampus Unsrat Kleak, Manado 95115, Indonesia
(3) Department of Medicine, Faculty of Medicine, Universitas Sam Ratulangi, Jl. Kampus Unsrat Kleak, Manado 95115, Indonesia
(*) Corresponding Author

Abstract

Kawangkoan peanut skin is a rich source of catechins, potent natural antioxidants with potential applications in pharmaceutical and cosmetic formulations. Reliable analytical methods are required to quantify catechin in complex delivery systems such as nanoemulsions. This study aimed to validate a UV-vis spectrophotometric method for catechin determination using a vanillin–H₂SO₄ reagent and to evaluate the antioxidant activity and physicochemical properties of a nanoemulsion containing Kawangkoan peanut skin extract. Method validation included linearity, accuracy, precision, limit of detection (LOD), and limit of quantification (LOQ). Antioxidant activity was evaluated using DPPH and ABTS assays. The method showed good linearity over the concentration range of 3–15 µg/mL (R = 0.9991), with LOD and LOQ of 0.4800 and 1.600 µg/mL, respectively. The nanoemulsion was clear, stable, and nanosized, with a particle size of 12.3 nm, zeta potential of −35 mV, and catechin entrapment efficiency of 74.77%. The nanoemulsion exhibited strong antioxidant activity. These results demonstrate that the validated UV-vis method is suitable for routine catechin analysis and support the potential application of catechin-loaded nanoemulsions as functional antioxidant ingredients.

Keywords

Kawangkoan peanut skin; catechin; nanoemulsion; antioxidant activity; UV-vis method validation

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DOI: https://doi.org/10.22146/ijc.115349