Antioxidant Activity of Moringa oleifera Extracts

Wiwit Denny Fitriana(1), Taslim Ersam(2), Kuniyoshi Shimizu(3), Sri Fatmawati(4*)

(1) Department of Chemistry, Sepuluh Nopember Institute of Technology (ITS), Jl. Arief Rahman Hakim, Sukolilo, Surabaya 60111 Faculty of Mathematics and Natural Sciences, Universitas Pesantren Tinggi Darul Ulum, Jombang 61481
(2) Department of Chemistry, Sepuluh Nopember Institute of Technology (ITS), Jl. Arief Rahman Hakim, Sukolilo, Surabaya 60111
(3) Department of Agro-environmental Science, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581
(4) Department of Chemistry, Sepuluh Nopember Institute of Technology (ITS), Jl. Arief Rahman Hakim, Sukolilo, Surabaya 60111
(*) Corresponding Author


Moringa oleifera have been evaluated for its antioxidant activity. M. oleifera leaves were extracted with methanol, ethyl acetate, dichloromethane and n-hexane. The antioxidant activity of extracts were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity assay and an improved 2,2’-azino-bis-[3-ethylbenzothiazoline sulphonate] (ABTS) radical cation decolorization assay in vitro. Trolox was used as standard with IC50 5.89 μg/mL in DPPH assay and 3.06 μg/mL in ABTS assay. The methanol extract showed the highest free radical scavenging activity with IC50 value of 49.30 μg/mL in DPPH assay and 11.73 μg/mL in ABTS assay. This study provided that M. oleifera leaves possess antioxidant.


Moringa oleifera leaves; antioxidant; DPPH; ABTS

Full Text:

Full Text Pdf


[1] Halliwell, B., 2008, Arch. Biochem. Biophys., 476 (2), 107–112.

[2] Johnson, I.T., 2007, Proc. Nutr. Soc., 66 (2), 207–215.

[3] Tomaino, A., Cimino, F., Zimbalatti, V., Venuti, V., Sulfaro, V., De Pasquale, A., and Saija, A., 2005, Food Chem., 89 (4), 549–554.

[4] Karadag, A., Ozcelik, B., and Saner, S., 2009, Food Anal. Methods, 2, 41–60.

[5] Martysiak-Żurowska, D., and Wenta, W., 2012, Acta Sci. Pol. Technol. Aliment., 11 (1), 83–89.

[6] Mbikay, M., 2012, Front. Pharmacol., 3, 1–12.

[7] Farooq, F., Rai, M., Tiwari, A., Khan, A.A., and Farooq, S., 2012, J. Med. Plants Res., 6 (27), 4368–4374.

[8] Arabshahi, D.S., Devi, D.V., and Urooj, A., 2007, Food Chem., 100 (3), 100–1105.

[9] Fuglie, L.J., 2001, “The Miracle Tree: Moringa oleifera: Natural Nutrition for the Tropics” in The Miracle Tree: The Multiple Attributes of Moringa, CTA Publisher, 172.

[10] Charoensin, S., 2014, J. Med. Plant Res., 8 (7), 318–325.

[11] Brand-Williams, W., Cuvelier, M. E. and Berset, C., 1995, LWT Food Sci. Technol., 28 (1), 25–30.

[12] Dudonńe, S., Vitrac, X., Coutière, P., Woillez, M. and Mérillon, J. M., 2009, J. Agric. Food Chem., 57 (5), 1768–1774.

[13] Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., and Rice-Evans, C., 1999, Free Radic. Biol. Med., 26 (9-10), 1231–1237.

[14] Santos, A.F., Argolo, A.C., Paiva, P.M., and Coelho, L.C., 2012, Phytother. Res., 26 (9), 1366–1370.

[15] Lisdawati, V., and Kardono, B.S., 2006, Media Litbang Kesehatan, 16 (4), 1–7.

[16] Chu, Y.H., Chang, C.L., and Hsu, H.F., 2000, J. Sci. Food Agric., 80 (5), 561–566.

[17] Bhoyar, M.S., Mishra, G.P., Naik, P.K., and Srivastava, R.B., 2011, Aust. J. Crop Sci., 5 (7), 912–919.


Article Metrics

Abstract views : 16527 | views : 27335

Copyright (c) 2016 Indonesian Journal of Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.


Indonesian Journal of Chemistry (ISSN 1411-9420 /e-ISSN 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

Analytics View The Statistics of Indones. J. Chem.