Major Anthocyanin Pigments in the  Ficus padana  Fruits: HPLC-DAD-ESI-MS Identification and Antioxidant Activity

Daimon Syukri; Djaswir Darwis; Adlis Santoni

doi:10.22146/ijc.21242

Major Anthocyanin Pigments in the Ficus padana Fruits: HPLC-DAD-ESI-MS Identification and Antioxidant Activity

https://doi.org/10.22146/ijc.21242

Daimon Syukri^(1*), Djaswir Darwis⁽²⁾, Adlis Santoni⁽³⁾

(1) Department of Agricultural Product Technology, Andalas University, Limau Manis, Padang 25163, West Sumatra
(2) Department of Chemistry, Andalas University, Limau Manis, Padang 25163, West Sumatra
(3) Department of Chemistry, Andalas University, Limau Manis, Padang 25163, West Sumatra
(*) Corresponding Author

Abstract

The anthocyanins in Ficus padana were extracted and identified by using high-performance liquid chromatography/diode array detection and electrospray ionization/mass spectrometry (HPLC-DAD-ESI-MS). The individual anthocyanins were identified by comparing between their mass spectral data and published data. The first compound (peak 1) was identify as a pelargonidin 3-(6”-p-coumarylglucoside)-5-(4”’-Malonylglucoside) and the second compound was identify as a pelargonidin 3-(6’’-Malonylglucoside). The second one is a major compound that taking up to 91% of the total anthocyanin content in F. padana extract. The antioxidant activity was determined with 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays and results showed that extract possessed high antioxidant capacity.

Keywords

Ficus padana; anthocyanin; HPLC-DAD-ESI-MS; antioxidant activity

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DOI: https://doi.org/10.22146/ijc.21242