THE POTENTIOMETRIC UREA BIOSENSOR USING CHITOSAN MEMBRANE

Ani Mulyasuryani; Anna Roosdiana; Arie Srihardyastutie

doi:10.22146/ijc.21454

THE POTENTIOMETRIC UREA BIOSENSOR USING CHITOSAN MEMBRANE

https://doi.org/10.22146/ijc.21454

Ani Mulyasuryani^(1*), Anna Roosdiana⁽²⁾, Arie Srihardyastutie⁽³⁾

(1) Chemistry Department, Brawijaya University Jalan Veteran, Malang
(2) Chemistry Department, Brawijaya University Jalan Veteran, Malang
(3) Chemistry Department, Brawijaya University Jalan Veteran, Malang
(*) Corresponding Author

Abstract

Potentiometric urea biosensor development is based on urea hydrolysis by urease resulted CO₂. The biosensor is used chitosan membrane and the H₃O⁺ electrode as a transducer. The research was studied of effecting pH and membrane thickness to the biosensor performance. The best biosensor performance resulted at pH = 7.3 and 0.2 mm of membrane thickness. The biosensor has a Nerntian factor 28.47 mV/decade; the concentration range is 0.1 up to 6.00 ppm; and the limit of detection is 0.073 ppm. The response time of this biosensor is 280 seconds, efficiency 32 samples and accuracy 94% up to 99%.

Keywords

biosensor; potentiometry; urea; chitosan membrane

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DOI: https://doi.org/10.22146/ijc.21454