Ani Mulyasuryani(1*), Anna Roosdiana(2), Arie Srihardyastutie(3)

(1) Chemistry Department, Brawijaya University Jalan Veteran, Malang
(2) Chemistry Department, Brawijaya University Jalan Veteran, Malang
(3) Chemistry Department, Brawijaya University Jalan Veteran, Malang
(*) Corresponding Author


Potentiometric urea biosensor development is based on urea hydrolysis by urease resulted CO2. The biosensor is used chitosan membrane and the H3O+ electrode as a transducer. The research was studied of effecting pH and membrane thickness to the biosensor performance. The best biosensor performance resulted at pH = 7.3 and 0.2 mm of membrane thickness. The biosensor has a Nerntian factor 28.47 mV/decade; the concentration range is 0.1 up to 6.00 ppm; and the limit of detection is 0.073 ppm. The response time of this biosensor is 280 seconds, efficiency 32 samples and accuracy 94% up to 99%.


biosensor; potentiometry; urea; chitosan membrane

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[1] Bennington, J.L., 1984, Dictionary and Encyclopedia of Laboratory Medicine and Technology, W.B. Saunders Co., Philadelphia.

[2] Egins, B.R., 2002, Chemical Sensors and Biosensors, John Wiley & Sons, LTD, Singapore.

[3] Chaplin, M., 2004, Potentiometric Biosensors, Faculty of Engineering, Science and The Built Environment, South Bank University, London.

[4] Wang, Y., Xu, H., Zhang, J., and Li, G., 2008, Sensors , 8, 2043–2081.

[5] Eggenstein, C., Borchardt, M., Diekmann, C., Gründig, B., Dumschat, C., Cammann, K., Knoll, M., and Spener, F., 1999, Biosens. Bioelectron, 14, 33–41.

[6] Pijanowska, D.G., Dawgul, M., and Torbich, W., 2003, Sensors, 3, 160–165.

[7] Grieshaber, D., MacKenzie, R., Vörös, J., and Reimhult, E., 2008, Sensors, 8, 1400–1458.

[8] Yahya, P.K.I., Julianto, A., Mulyasuryani, A., and Roosdiana, A., 2007, International Conference on Chemistry, UGM, Yogyakarta, Indonesia.

[9] Carroll, J.J., and Mather, A.E., 1992, J. Solution Chem., 21, 607–621.

[10] Ito, A., Sato, M., and Anma, T., 1997, Permeability of CO2 Through Chitosan Membrane Swollen by Water Vapor in Feed Gas, Department of Material and Chemical Engineering, Niigata University, Japan.

[11] Mulyasuryani, A., Roosdiana, A., Sutrisno, and Srihardyastutie, A., 2008, The 2nd Penang International Conference for Young Chemists, Penang, Malaysia.

[12] Krajewska, B., Leszko, M., and Zaborska, W., 1990, Urease Immobilized on Chitosan Membrane: Preparation and Properties,

[13] Wang, J., 2001, Analytical Electrochemistry, 2rd ed., VCH Publisher, Inc., 133.

[14] Gu, Z.Y., Xue, and Li,, 2001, Polym. Adv. Tech., 53, 665-669.

[15] Magalhães, J.M.C.S., and Machado, A.A.S.C., 2002, Analyst, 127, 1400–1458.

[16] Srivastava, P.K., Arvind, M.K., and Srinivasan, 2001, Biotechnol. Appl. Biochem., 34, 55–62.


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