IMMOBILIZATION OF PAPAIN ON CHITOSAN

https://doi.org/10.22146/ijc.21609

Sari Edi Cahyaningrum(1*), Narsito Narsito(2), Sri Juari Santoso(3), Rudiana Agustini(4)

(1) Chemistry Department, Faculty of Mathematics and Natural Sciences Surabaya State University, Jl. Ketintang, Surabaya 60231
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281
(3) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281
(4) Chemistry Department, Faculty of Mathematics and Natural Sciences Surabaya State University, Jl. Ketintang, Surabaya 60231
(*) Corresponding Author

Abstract


In this study, papain was immobilized on chitosan with Mg(II) cosslinked agent. Studies on free and immobilized papain systems for determination of optimum pH, optimum temperatur, thermal stability and reusability were carried out. The results showed that free papain had optimum pH 6.5 and optimum temperature 55 °C while the immobile papain hadoptimum pH 8 and optimum temperature 80 °C. The thermal stability of the immobilized papain, relative to that of the free papain, was markedly increased. The immobilized papain can be reused for at least six times.


Keywords


papain; immobilization; chitosan

Full Text:

Full Text Pdf


References

[1] Godfrey, T., and Reichet, J., 1986, Industrial Enzymology, The Application of Enzyme in Industry, Great Britain, Stockton Press.

[2] Bradford, M., 1976, Anal. Biochem., 72, 248-254.

[3] Chibata, I., 1978, Immobilixe Enzymes Research and Development, John and Wiley and Sons, New York.

[4] Afaq, S., 2001, Electronic J. Biotechnology, Catolica de Velparaaiso Chile, 6, 234-237.

[5] Yagar, Y., 2002, J. Process Biochem., 31l, 287-289.

[6] Jiannmin, J., 2006, Biological Macromolecular, 39, 185-191.

[7] Hong-Meyers, S.P., 1989, J. Agric. Food. Chem., 37, 580-583.

[8] Taboada, L., Cabrera, G., and Cardenas, G., 2002, J. Chil. Chem. Soc., 48, 1-15

[9] Tien, C.L., Millette, M., Mateescu, M.A., and Lacroix, M., 2004, Biotechnol. Appl. Biochem., 39, 347-354



DOI: https://doi.org/10.22146/ijc.21609

Article Metrics

Abstract views : 2237 | views : 5010


Copyright (c) 2010 Indonesian Journal of Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 


Indonesian Journal of Chemistry (ISSN 1411-9420 /e-ISSN 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

Web
Analytics View The Statistics of Indones. J. Chem.