Teuku Beuna Bardant(1*), Kiky Corneliasari Sembiring(2), Achmad Hanafi Setiawan(3)

(1) Research Center for Chemistry – Indonesian Institute of Sciences Kawasan PUSPIPTEK Serpong, Tangerang Indonesia 15314
(2) Research Center for Chemistry – Indonesian Institute of Sciences Kawasan PUSPIPTEK Serpong, Tangerang Indonesia 15314
(3) Research Center for Chemistry – Indonesian Institute of Sciences Kawasan PUSPIPTEK Serpong, Tangerang Indonesia 15314
(*) Corresponding Author


The goal of these preliminary studies is to support Indonesian program for increasing palm oil added value through independent production technology based on Indonesian natural resources. Various palm oil derivatives could be synthesized enzymatically using lipase from microbes that available in Indonesia. Tempe's mould is available in abundance in Indonesia and had already been proved for producing lipase. This paper provides information about producing crude lipase from Tempe's mould cultivated in rice-husk-based solid media using palm oil as carbon source. Observed variables include solid media composition, optimum fermentation time, extraction and enriching process of crude lipase. The crude lipase was analyzed its hydrolysis activity on coconut oil and palm oil. The result of these preliminary studies shows that this production process is a simple and tough process and very potential to be developed.


lipase; Tempe's mould; palm oil; solid fermentation; rice husk

Full Text:

Full Text PDF


[1] Iwai, M., Tsujisaka, Y., Itaya, K., Okamoto, Y. and Fukamoto, J. 1966, Kagaku to Kogyo, 40, 80-89.

[2] Iwai, M. 1963, Bulletin of the Osaka Municipal Technical research Institute. 39, 63-70.

[3] Goodman, J.J. 1950, Science. 112, 176-179.

[4] Iwai, M. and Tsujisaka, Y. 1966, The Purification and The Properties of The Three Kinds of Lipase from Rhizopus delemar. Agric. Biol. Chem. 38, 1241-1247.

[5] Beisson, F., Tiss, A., Riviere, C., Verger, R. 2000, Eur. J Lipid Sci. & Tech. 133-153.

[6] Nuraida, L., Hariyadi, P., Dewanti, R., and Budiyanto, S. 2000, Production of Lipase with Esterification Activity from Moulds Isolated from Indonesian Fermented Foods. IFT Annual Meeting - New Orleans, Louisiana.

[7] Abbas, H., Hiol A., Deyris V., and Comeau L., 2002, Enzyme & Microbial Tech., 31, 968–975.

[8] Iwai, M., Tsujisaka, Y., Okumura, S., and Katsumoto H., 1966, Yukagaku, 29, 587-591.

[9] Rajasekharan, R. and Kanchan, B., 2002, A Process of Isolation and Utilization of Rice Bran Lipase. Patent number(s): WO02101033 A1. Patent assignee(s): Indian Institute of Science;CB Bio Products India Pvt. Ltd.

[10] Razak C.N.A., Salleh A.B., Musani R., Samad M.Y., and Basri M., 1997, J Molec. Catal. B: Enzymatic. 3, 153-159

[11] Hiol, A., Marie, J. R. Nathalie, Druet, D., Sarda L., and Claude L., 2000, Enzyme & Microbial Tech., 26, 421–430.

[12] Koblitz, G. M. B., Pastore, G. M. 2006, Ciênc. agrotec., Lavras. 30, 3, 494-502.

[13] Lee, D.W., 2001, Enzyme & Microbial Tech. 29, 363–371.

[14] Hatzinikolaou, D. G., Kourentzi, E., Stamatis, H., Christakopoulos, P., Kolisis, R.N., Kekos, D., and Macris, B. J. 1999. J. Biosci. & Bioeng. 88, 1, 53-56.


Article Metrics

Abstract views : 1062 | views : 1023

Copyright (c) 2010 Indonesian Journal of Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.


Indonesian Journal of Chemistry (ISSN 1411-9420 / 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

Analytics View The Statistics of Indones. J. Chem.