Pipin T. Kurniawati(1*), H. Soetjipto(2), Leenawati Limantara(3)

(1) Satya Wacana Christian University, Jl. Diponegoro 52-61 Salatiga 50711
(2) Satya Wacana Christian University, Jl. Diponegoro 52-61 Salatiga 50711
(3) Workstation of Mochtar Riady Institute for Nanotechnology, Lippo Karawaci
(*) Corresponding Author


Research on Bixa orellana L. have been done to isolate, identify and determine bixin percentage, the antioxidant and antibacterial activities of bixin from B. orellana seed.  Isolation and identification of bixin was done by thin layer chromatography (TLC), column chromatography, chemical test of bixin and UV-Vis double beam spectroscopy. Percentage of bixin was calculated by JECFA method, the antioxidant activity was determined by DPPH (1-1 diphynilpicrylhidrazil) method while antibacterial activity was analyzed by the use of agar diffusion method. Thin layer chromatography (TLC) for the crude extract contained 5 spot, where spot 5th was bixin. Bixa orellana has 75±3% of bixin. Antioxidant activity of bixin had IC50 548.5±20.0 ppm. Whereas the antibacterial activity of bixin against the Escherichia coli and Staphylococus aureus could be classified as weak inhibition category at 500-750 μg and medium inhibition category at 1500 μg.


Bixa orellana L.; bixin; antioxidant; antibacteria

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[1] Djarismawati, Sugiharti, and Naingolan, 2004, Jurnal Ekologi Kesehatan., 3, 7-12

[2] Mckeown, G.G., 1963, J. Assoc. Offic. Agr. Chemists, 46(5), 6-790

[3] Wurts, M.L and Torreblanca, R.A., 1983, Archivos Latinoamericanos de Nutricion, 33(3), 19-606

[4] Francis, F.J., 2000, Cereal Foods World, 45, 198-203

[5] Patil, J. and Balasubramaniyan,V., 2001, Int. J. of Pharma. Excipients, 3(4), 89-93

[6] Nishizawa, M., Chonan, T., Sekijo, I., and Suggi, T., 1983, Hokkaidoritsu Eisei kenkyushoho, 33, 28-34

[7] Hirata, K., Hirokado, M., Uematsu, Y., Nakajima, K., and Kazama, M., 1989, Tokyo-toritsu Eisei Kenkyusho kenkyu Nenpo, 40, 82-178

[8] Tricard, C., Cazabeil, J.M., and Bernard, 1998, Sciences des Aliments, 18(1), 25-40

[9] Gulrajani, M.L., Gupta, D., and Maulik, S.R., 1999, Ind. J. Fibre & Textile Res., 24(2), 131-135

[10] Levy, L.W. and Rivadeneira, D.M., 2000, Annatto, IFT Basic Symposium Series, 14, 115-152

[11] Tan, Y., 1987, Bixin, Shipin Kexue, 86, 6-8

[12] Alves, R.W., Jauregi P., Ulson de Souza A.A., and Ulson de Souza S.M.A.G., 2005, Recovery of norbixin using colloidal gas aphrons (CGAS), 2nd Merosur Congress on Chemical Engineering dan 4nd Merosur Congress on Process Engineering, (1-9).

[13] Toledo De Oliveira T., Nagem T.J., Rocha Da Costa M., Marciano Da costa L., Magalhaes N.M., Stringheta P.C., Queiroga De Lima E., Kling De moraes G.H., and Da Silva H., 2004, Ars Pharma., 45(1), 5-20

[14] Galindo-Cuspinera, Giusti, M.M., and Rankin, S.A., 2003, Bioautography and Characterization of the Antimicrobial Compounds Present in commercial Annatto Extracts, Food Chemistry: Enzymes, vitamins and plant pigments, Session 92A-2.

[15] Britton, G., Liaaen-Jensen, S., and Pfander, H., 1982, Carotenoids: Isolation and Analysis, Birkhäuser Verlog Basel. Boston. Berlin, 1A. 93-94.

[16] JECFA, 1992, Annatto Extracts, FNP 25 dan FNP 52.

[17] Panovska, T., Kulevanova, S., and Stefova, M., 2005, Acta Pharm., 55, 207-214

[18] Atoui, A.K., Mansouri, A., Boskou, G. and Kefalas, P., 2005, J. Food Chem., 8, 27-36

[19] Krinsky, N.I., 1979, Pure appl. Chem., 51, 649-660.

[20] Krinsky, N.I., 1989, Beta-carotene: Function, In New Protective Roles for Selected Nutrients, G.A. Spiller dan J. Scala, eds. Alan R. Liss. New York. Pp. 1-5.

[21] Yanishlieva-Maslarova, N.V., 2001. 3: Inhibiting Oxidation, Woodhead Publishing Ltd and CRC Press LLC. 22-70, in Pokorny, J., Yanishlieva, N., dan Gordon, M., 2003, Antioxidant in Food Practical apllication, Woodhead Publishing Ltd and CRC Press LLC.

[22] Trilaksani, W., 2003, Antioksidan: Jenis, Sumber, Mekanisme Kerja dan Peran terhadap Kesehatan, Term paper, Intoductory Science Phylosophy (PPS702), Graduate Program/S3, Institut Pertanian Bogor.

[23] Gordon, M.H., 1990, The Mechanism of Antioxidants Action In Vitro, in : B.J.F. Husdson, aditor. Food Antioxidant, Elsivier Applied Science, London.

[24] Karyadi, E., 1997, Antioksidan Resep Sehat dan Umur Panjang, Universitas Indonesia Press, Jakarta.

[25] Plectzar, J.M. and Chan, E.C.S., 1998, Dasar-dasar Mikrobiologi, Edisi kedua, Universitas Indonesia Press, Jakarta

[26] Proestos, C., Dozarus, I.S., Nychar, G.J.E., and Komaitis, M., 2005, J. Food Chem., 95, 664-671


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