* Corresponding Author
INTERACTION OF IRON(II) MIXED-LIGAND COMPLEXES WITH DNA: BASE-PAIR SPECIFICITY AND THERMAL DENATURATION STUDIES
https://doi.org/10.22146/ijc.21849 Mudasir Mudasir(1*), Karna Wijaya(2), Endang Tri Wahyuni(3), Hidenari Inoue(4)
(1) Chemistry Department, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Yogyakarta
(2) Chemistry Department, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Yogyakarta
(3) Chemistry Department, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Yogyakarta
(4) Department of applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku,Yokohama 223-8522
(*) Corresponding Author
Abstract
A research about base-pair specificity of the DNA binding of [Fe(phen)3]2+, [Fe(phen)2(dip)]2+ and [Fe(phen)(dip)2]2+ complexes and the effect of calf-thymus DNA (ct-DNA) binding of these metal complexes on thermal denaturation of ct-DNA has been carried out. This research is intended to evaluate the preferential binding of the complexes to the sequence of DNA (A-T or G-C sequence) and to investigate the binding strength and mode upon their interaction with DNA. Base-pair specificity of the DNA binding of the complexes was determined by comparing the equilibrium binding constant (Kb) of each complex to polysynthetic DNA that contain only A-T or G-C sequence. The Kb value of the interaction was determined by spectrophotometric titration and thermal denaturation temperature (Tm) was determined by monitoring the absorbance of the mixture solution of each complex and ct-DNA at λ =260 nm as temperature was elevated in the range of 25 - 100 oC. Results of the study show that in general all iron(II) complexes studied exhibit a base-pair specificity in their DNA binding to prefer the relatively facile A-T sequence as compared to the G-C one. The thermal denaturation experiments have demonstrated that Fe(phen)3]2+ and [Fe(phen)2(dip)]2+ interact weakly with double helical DNA via electrostatic interaction as indicated by insignificant changes in melting temperature, whereas [Fe(phen)2(dip)]2+ most probably binds to DNA in mixed modes of interaction, i.e.: intercalation and electrostatic interaction. This conclusion is based on the fact that the binding of [Fe(phen)2(dip)]2+ to ct-DNA moderately increase the Tm value of ct- DNA
Keywords
Full Text:
Full Text PdfReferences
[1] Lerman, L.S., 1961, J. Mol. Biol., 3,18.
[2] Chow, C. S. and Bogdan, F. M., 1997, Chem. Rev. 97, 1489.
[3] Schilt, A. A. and Taylor, R. C. 1959, J. Inorg. Nucl. Chem., 9, 211.
[4] Mudasir, Yoshioka, N., and Inoue, H., 1999, Transition Met. Chem., 24, 210.
[5] Well, R. D., J. E. Larson, R. C. Shortle and C. R. Cantor, 1970, J. Mol. Biol. 54, 465.
[6] Liu, F., Meadows, K.A. and McMillin, D.R. 1993, J. Am. Chem. Soc., 115, 6699.
[7] Schmechel, D.E.V. and Crothers, D.M., 1971, Biopolymers, 10, 465.
[8] Mudasir, Yoshioka, N., and Inoue, H., 1999, J. Inorg. Biochem., 77, 239.
[9] Lincoln, P. and Norden, B., 1998, J. Phys. Chem. B, 102 , 9583.
[10] Choi, S-D., Kim, M-S., Kim, S. K., Tuite, E. and Norden, B., 1997, Biochemistry, 36, 214.
[11] Nair, R. B., Teng, E. S., Kirk, S. L., and Murphy, C. J., 1998, Inorg. Chem., 37, and refs. there in.
DOI: https://doi.org/10.22146/ijc.21849
Article Metrics
Abstract views : 1339
| 
views : 2139
Copyright (c) 2010 Indonesian Journal of Chemistry
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Indonesian Journal of Chemistry (ISSN 1411-9420 /e-ISSN 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.