Storage and Condition of Biomass Influence to Biosorption of Lead (II) and Zinc(II) by Saccharomyces cerevisiae Biomass

Jasmidi Jasmidi(1), Eko Sugiharto(2*), Mudjiran Mudjiran(3)

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Faculty of Education Medan
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara PO BOX BLS 21 Yogyakarta 55281
(3) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara PO BOX BLS 21 Yogyakarta 55281
(*) Corresponding Author


The influence of length and condition of Biomass Storage on the biosorption of lead and zinc that present together in a solution by Saccharomyces cerevisiae biomass were studied. In this experiment, variables of length and condition of biomass storage were examined. Concentration of lead and zinc were determined by atomic absorption spectrophotometric (AAS) using air-acetilene as atomizing flame. Loading of lead and zinc on the biomass were determined as the difference between the initial and the final concentration of lead and zinc in the solution. Biosorption of lead and zinc were influenced by condition and storage of the biomass. Storage of biomass in the room temperature for one week cause an increasing uptake. Storage for longer period result in decrease of lead and zinc uptake. Storage of biomass in a freezer up to 2 weeks increased the uptake of lead, but did not influence the uptake of zinc. Storage for longer period decreased the uptake of both of lead and zinc. For all condition the uptake of lead higher than the uptake of zinc by Saccharomyces cerevisiae.


biosorption; lead; zinc; Saccharomyces cerevisiae

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[1] Avery, S.V., and Tobin, Z.M., 1992, Mecanism of Strontium Uptake by Laboratory and Brewing strains of Saccaromyces cerevisiae, Appl. Environ. Microbiol., 58: 3883-3889.

[2] Darmono., 1995, logam Dalam Sistem Biologi Makhluk Hidup, UI-Press, Jakarta.

[3] Dube, H.C., 1978. Fungi, Bacteria and Viruses, Vikas Publishing House PUT LTD, New Delhi.

[4] Gadd, G.M., 1992, Microbial Control of Heavy Metal Pollution, In Microbial Control of Pollution, Fry, J.C., Gadd, G.M., Herbert, R.A., Jones, R.W., and Watson-Craik, I.A., (eds), Society for General Microbiology Symposium, Cambridge University Press, UK, pp. 5988.

[5] Hancock, I.C., 1996, Bioremediation of Heavy Metal Pollution Possibilities and Practicalities, The Current Position, In Symposium and Workshop on Heavy Metal Bioaccumulation. IUC Biotechnology, Gadjah Mada University, Yogyakarta, September, 18-20, 1998.

[6] Hough, J.S., Stevens, R., and Young, T.W., 1982, Malting and Brewing Science, Vol. II Hopped Wart and Beer, Second Edition, Chapman and Hall, London.

[7] Hughes, M.N., and Poole, R.K., 1989, Meths and Microorganisms, Chapman and Hall, London.

[8] Mahan, C.A., Majidi, V., and Holcombe, J.A., 1989, Evaluation of The Metal Uptake of Several Algae Starins in a Multicomponen Matrix Utilizing Inductivelly Coupled Plasma Emmision Spectrometry for Trace Metal Preconcentration, Anal. Chem., 61: 624627.

[9] Pelczar, Jr., M.J., Reid, R.D., and Chan, E.C.S., 1976, Elements of Microbiology, Mc. Graw-Hill Book Company, New York.

[10] Sanderson, R.T., 1964, Principles of Chemical Reaction, J. of Chem. Ed., 41: 13-22.

[11] Seinko, M.J., and Plane, R.A., 1996, Chemistry: Principlesand Properties, Mc. Grew-Hill Book Company, New York.

[12] Strandberg, G.W., Shumate II, S.E., and Parrot, Jr., J.R., 1981, Microbial Cells as Biosorbents for heavy Metals: Accumulation of Uranium by Saccaromyces cerevisiae and Pseudomanas aeruginosa, Appl. Environ. Microbiol., 41: 237-245.

[13] Volesky, B., and May Phillips, H.A., 1995, Biosorption of Heavy Metal by Saccaromyces cerevisiae, Appl. Microbiol. Biotechnol., 42:797-806.


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Indonesian Journal of Chemistry (ISSN 1411-9420 /e-ISSN 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

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