Isolation, Cellulase Activity Test and Molecular Identification of Selected Cellulolytic Bacteria Indigenous Rice Bran

Akyunul Jannah; Aulanni`am Aulanni`am; Tri Ardyati; Suharjono Suharjono

doi:10.22146/ijc.26783

Isolation, Cellulase Activity Test and Molecular Identification of Selected Cellulolytic Bacteria Indigenous Rice Bran

https://doi.org/10.22146/ijc.26783

Akyunul Jannah^(1*), Aulanni`am Aulanni`am⁽²⁾, Tri Ardyati⁽³⁾, Suharjono Suharjono⁽⁴⁾

(1) Department of Chemistry, Maulana Malik Ibrahim State Islamic University Malang, Jl. Gajayana 50 Dinoyo, Malang 65144, East Java, Indonesia
(2) Faculty of Veterinary Medicine, Brawijaya University, Jl. Puncak Dieng Dau, Malang 65151, East Java, Indonesia
(3) Department of Biology, Brawijaya University, Jl. Veteran 6, Malang 65144, East Java, Indonesia
(4) Department of Biology, Brawijaya University, Jl. Veteran 6, Malang 65144, East Java, Indonesia
(*) Corresponding Author

Abstract

Rice bran is the waste product of rice milling which is abundant in Indonesia, it can be used as a raw material for the manufacture of bioethanol by fermentation. Before being fermented, rice bran must be hydrolyzed into glucose by biomass degrading. This study was aimed to isolate indigenous cellulolytic bacteria from rice bran as producer of cellulolytic enzymes and resulted in 22 bacterial isolates that demonstrated cellulolytic activity being identified. Among them, BE 8 and BE 14 isolates showed the highest endoglucanase activity at 2.16 and 1.31 U/mL respectively. Identification of the 16S rDNA showed that BE 8 belongs to Bacillus subtilis and BE 14 in Bacillus cereus.

Keywords

cellulase enzyme; rice bran; 16S rDNA method; Bacillus subtillis; Bacillus cereus

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DOI: https://doi.org/10.22146/ijc.26783