Effect of Oxidation Agents on Photo-Decolorization of Vitamin B12 in the Presence of ZnO/UV-A System


Luma Majeed Ahmed(1*), Shaymaa Ibrahim Saaed(2), Ashraff Aziz Marhoon(3)

(1) Department of Chemistry, College of Science, University of Kerbala
(2) Department of Chemistry, College of Science, University of Kerbala
(3) Department of Chemistry, College of Science, University of Kerbala
(*) Corresponding Author


The aim of this work was to apply the many various oxidation agents namely H2O2, K2S2O8 and Fe2+ on the aqueous solution of vitamin B12 with the presence of ZnO. The results indicated that the use of the mixture of H2O2 and K2S2O8 in suspension of vitamin B12 and ZnO gave a maximum efficiency percentage about 95.85% in 12 min; it was higher than that without the addition of oxidation reagent 79.33% in the same time. The obtained results demonstrated that the activation energy for this photoreaction without the addition of oxidation agent was more than that the apparent activation energy value with the used mixture of H2O2 and K2S2O8. The thermodynamic study showed that both reactions were endothermic, less-random and non-spontaneous. The ΔH# with the oxidant agents (31.43 kJ mol-1) is less than that without using oxidant agents (35.81 kJ mol-1). Moreover, the addition of series of oxidant agent solution to vitamin B12 solution led to change the photocatalytic activity for decolorization of vitamin B12 in suspension solution of ZnO, and was found the activity sequence as follows: (H2O2 + K2S2O8) > (H2O2 + Fe(II)) (Fenton reaction) > (H2O2) > (K2S2O8) > (without the oxidation agents) > (K2S2O8 + Fe(II)) > Fe(II).


vitamin B12; cyanocobalamin; cobalamin; decolorization; oxidant

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[1] Her Majesty's Stationery Office, 1980, British pharmacopoeia 1980, Atlantic House, Holborn Viaduct, London EC1P 1BN, England.

[2] Combs, G.F., and McClung, J.P., 2017, The Vitamins, Fundamental Aspects in Nutrition and Health, 5th ed., Elsevier, Academic Press, Amsterdam.

[3] Navarra, T., 2004, The Encyclopedia of Vitamins, Minerals and Supplements, 2nd ed., Facts on File, Inc., USA.

[4] Beck, W.S., 2001, "Cobalamin (Vitamin B12)" in Handbook of Vitamins, 3rd ed., Eds. Rucker, R.B., Suttie, J.W., McCormick, D.B., and Machlin, L.J., Marcel Dekker, Inc., New York, CH 13.

[5] Green, R., and Miller, J.W., 2007, "Vitamin B12" in Handbook of Vitamins, 4th ed., Eds. Zempleni, J., Rucker, R.B., McCormick, D.B., and Suttie, J.W., CRC Press, Taylor & Francis Group, USA,CH 13.

[6] Combs, G.F., 2008, The Vitamins, Fundamental Aspects in Nutrition and Health, 3rd ed., Elsevier Academic Press, USA.

[7] Jaouen, G., Beck, W., and McGlinchey, M.J., 2006, "A Novel Field of Research: Bioorganometallic Chemistry, Origins, and Founding Principles" in Bioorganometallics: Biomolecules, Labeling, Medicine, Eds. Jaouen, G., Wiley-VCH, Weinheim, Germany,CH 1.

[8] Halbus A.F., and Hussein, F.H., 2012, Heterogeneous photocatalytic decolorization of cobalamin in the presence of aqueous titanium dioxide suspensions, Asian J. Chem., 24 (12), 1–3.

[9] Hussein, F.H., and Halbus, A.F., 2012, Rapid decolorization of cobalamin, Int. J. Photoenergy, 2012, 495435, 1–9.

[10] Halbus, A.F., and Hussein, F.H., 2012, Photocatalytic degradation of cobalamin by metalized TiO2, JUBPAS, 22 (1), 445–453.

[11] Quaroni, L., Reglinski, J., and Smith, W.E., 1995, Surface enhanced resonance Raman scattering from cyanocobalamin and 5'-deoxyadenosylcobalamin, J. Raman Spectros., 26, 1075–1076.

[12] Ru, E.L., and Etchegoin, P., 2009, Principles of Surface-Enhanced Raman Spectroscopy, Elsevier, UK.

[13] Seetharam, B., and Alpers, D., 1982, Absorption and transport of cobalamin (Vitamin B12), Annu. Rev. Nutr., 2 (3), 43–69.

[14] Hashshama, S.A., and Freedman, D.L., 2003, Adsorption of vitamin B12 to alumina, kaolinite, sand and sandy soil, Water Res., 37, 3189–3193.

[15] Poonawalla, F.M., and Iyengar, M.R.S., 1965, Microbiological assay of vitamin B12 in the presence of tetracycline, Appl. Microbiol., 13 (5), 755–756.

[16] Chen, P., Wolf, W.R., Castanheira, I., and Sanches-Silva, A., 2010, A LC/UV/Vis method for determination of cyanocobalamin (VB12) in multivitamin dietary supplements with on-line sample clean-up, Anal. Methods, 2 (8), 1171–1175.

[17] Kłaczkow, G., and Anuszewska, E.L., 1998, Analysis of cyanocobalamin in multivitamin preparation by high pressure liquid chromatography, Acta Pol. Pharm., 55 (3), 189–192.

[18] Ahmed, S., 2004, Photo electrochemical study of ferrioxalate actinometry at a glassy carbon electrode, J. Photochem. Photobiol., A, 161 (2-3), 151–154.

[19] Mahammed, B.A., and Ahmed, L.M., 2017, Enhanced photocatalytic properties of pure and Cr-modified ZnS powders synthesized by precipitation method, J. Geosci. Environ. Prot., 5 (10), 101–111.

[20] Usman, M., Noviyanti, A., and Eddy, D., 2017, Photocatalytic degradation of diazinon using titanium oxide synthesized by alkaline solvent, Indones. J. Chem., 17 (1), 22–29.

[21] Eesa, M.T., Juda, A.M., and Ahmed, L.M., 2016, Kinetic and thermodynamic study of the photocatalytic decolourization of light green SF yellowish (acid green 5) dye using commercial bulk titania and commercial nanotitania, IJSR, 5 (11), 1495–1500.

[22] Abbas, S.K., and Ahmed, L.M., 2018, UV-A light induced for photo-decolorization of chlorazol black BH dye by zinc oxide suspensions in neutral medium, J. Arch. Chem. Res., 1 (1), 1–4.

[23] Mousavi, S.A., and Nazari, S., 2017, Applying response surface methodology to optimize the fenton oxidation process in the removal of reactive red 2, Pol. J. Environ. Stud., 26 (2), 765–772.

[24] Abdollahi, Y., Abdullah, A.H., Zainal, Z., and Yusof, N.A., 2012, Photocatalytic degradation of p-cresol by zinc oxide under UV irradiation, Int. J. Mol. Sci., 13 (1), 302–315.

[25] Moore, J.C., Louder, R., and Thompson, C.V., 2014, Photocatalytic activity and stability of porous polycrystalline ZnO thin-films grown via a two-step thermal oxidation process, Coatings, 4 (3), 651–669.

[26] Hayon, E., Treinin, A., and Wilf, J., 1972, Electronic spectra, photochemistry, and autoxidation mechanism of the sulfite-bisulfite-pyrosulfite systems. SO2-, SO3-, SO4-, and SO5- radicals, J. Am. Chem. Soc., 94 (1), 47–57.

[27] Eberson, L., 1982, Electron-transfer reactions in organic chemistry, Adv. Phys. Org. Chem., 18, 79–185.

[28] Song, S., Xu, L., He, Z., Chen, J., Xiao, X., and Yan, B., 2007, Mechanism of the photocatalytic degradation of C.I. Reactive Black 5 at pH 12.0 using SrTiO3/CeO2 as the catalyst, Environ. Sci. Technol., 41 (6), 5846–5853.

[29] Sauer, T., Neto, G.C., José, H.J., and Moreira, R.F.P.M., 2002, Kinetics of photocatalytic degradation of reactive dyes in a TiO2 slurry reactor, J. Photochem. Photobiol., A, 149 (1-3), 147–154.

[30] Pérez-Sicairos, S., Corrales-López, K.A., Hernández-Calderón, Ó.M., Salazar-Gastélum, M.I., and Félix-Navarro, R.M., 2016, Photochemical degradation of nitrobenzene by S2O8–2 ions and UV radiation, Rev. Int. Contam. Ambie., 32 (2), 227–236.

[31] Goharshadi, E.K., Hadadian, M., Karimi, M., and Azizi-Toupkanloo, H., 2013, Photocatalytic degradation of reactive black 5 azo dye by zinc sulfide quantum dots prepared by a sonochemical method, Mater. Sci. Semicond. Process., 16 (4), 1109–1116.

[32] Mashkour, M., Al-Kaim, A., Ahmed, L., and Hussein, F.H., 2001, Zinc oxide assisted photocatalytic decolorization of Reactive Red 2 dye, Int. J. Chem. Sci., 9 (3), 969–979.

[33] Pare, B., Jonnalagadda, S.B., Tomar, H., Singh P., and Bhagwat, V.W., 2008, ZnO assisted photocatalytic degradation of acridine orange in aqueous solution using visible irradiation, Desalination, 232 (1-3), 80–90.

[34] Hashemian, S., Tabatabaee, M., and Gafari, M., 2013, Fenton oxidation of methyl violet in aqueous solution, J. Chem., 2013, 509097, 1–6.

[35] Wang, C., Liu H., and Sun, Z., 2012, Heterogeneous photo-fenton reaction catalyzed by nanosized iron oxides for water treatment, Int. J. Photoenergy, 2012, 801694, 1–10.

[36] Kim, M.I., Im, J.S., In, S.J., Kim, H., Kim, J.G., and Lee, Y.S., 2008, Improved photo degradation of rhodamine B dye using iron oxide/carbon nanocomposite by photo-fenton reaction, Carbon Lett., 9 ( 3),195–199.

[37] Bahnemann, D., 1999, "Photocatalytic Detoxification of Polluted Waters" in Environmental Photochemistry. The Handbook of Environmental Chemistry, Reactions and Processes, vol. 2, Eds. Hutzinger, O., Springer-Verlag Berlin Heidelberg GmbH, 285–351.

[38] Einschlag, F.S.G., Braun, A.M., and Oliveros, E., 2015, “Fundamentals and Applications of the Photo-Fenton Process to Water Treatment” in Environmental Photochemistry Part III, Eds. Bahnemann, D.W., and Robertson, P.K.J., Springer-Verlag Berlin Heidelberg, 301–342

[39] Ahmed, L.M., and Hussein, F.H., 2014, Roles of Photocatalytic Reactions of Platinized TiO2 Nanoparticales, LAP Lambert Academia Published, Germany.

[40] Zuafuani, S.I., and Ahmed, L.M., 2015, Photocatalytic decolourization of direct orange Dye by zinc oxide under UV irradiation, Int. J. Chem. Sci., 13 (1), 187–196.

[41] Fathal, E.S., and Ahmed, L.M., 2015, Optimization of photocatalytic decolourization of methyl green dye using commercial zinc oxide as catalyst, Kerb. J., 13 (1), 53–63.

[42] Ahmed, L. M., Hussein, F.H., and Mahdi, A.A., 2012, Photocatalytic dehydrogenation of aqueous methanol solution by naked and platinized TiO2 nanoparticles, Asian J. Chem., 24 (12), 5564–5568.

[43] Ahmed, L.M., and Hussein, F.H., 2012, Quantum yield of formaldehyde formation from methanol in the presence of TiO2 and platinized TiO2 photocatalysts, JUBPAS, 22 (1), 464–470.

[44] Ahmed, L.M., Tawfeeq, F.T., Abed Al-Ameer, M.H., Abed Al-Hussein, K., and Athaab, A.R., 2016, Photo-degradation of reactive yellow 14 dye (a textile dye) employing ZnO as photocatalyst, GEP, 4, 34–44.

DOI: https://doi.org/10.22146/ijc.33470

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