Optimization of o-cresol degrading microorganism and kinetics of degradation

Krishnan Nhattuketty Shainy; Rajamanickam Usha

doi:10.22146/ijc.35326

Optimization of o-cresol degrading microorganism and kinetics of degradation

https://doi.org/10.22146/ijc.35326

Krishnan Nhattuketty Shainy⁽¹⁾, Rajamanickam Usha^(2*)

(1) Department of Microbiology, Safi Center for Scientific Research, Vazhayoor East, Malappuram – 673633, Kerala, India
(2) Department of Microbiology, Karpagam Academy for Higher Education, Eachinary, Coimbatore – 641021, Tamil Nadu, India
(*) Corresponding Author

Abstract

In the present study, Pseudomonas monteilii CR13 isolated from petroleum contaminated soil demonstrated the highest specific o-cresol degradation rate at all tested o-cresol concentrations and also was not disturbed by the starting substrate concentration used (o-cresol-500 mg/L). After a serial transfer of the isolate into a series of increasing o-cresol level, the organism demonstrated significant improvement on degradation ability up to 3000 mg/L. The optimum condition for the cell mass increase and biodegradation of o-cresol by Pseudomonas monteilii was in the minimal mineral medium of 3 at a pH of 6.5 and temperature 30 °C, stirring velocity of 160 rpm, and the substrate concentration of 500 mg/L. The biodegradation kinetic study was carried out by bacteria in different initial substrate concentrations (500–3000 mg/L). In the present test the μ_max, K_s and the μ were found 0.332 h^-1, 0.166 mg/L and 0.0282 mg/L for 500 mg/L of o-cresol, respectively. The organism is highly promising and could be used to remove high concentrations of o-cresol from highly polluted aquatic and soil regions. The cells could be immobilized on a suitable matrix and the efficiency of degradation could be effectively improved.

Keywords

biodegradation; o-cresol; Pseudomonas monteilii; petroleum contaminated soil

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DOI: https://doi.org/10.22146/ijc.35326