Angiotensin Converting Enzyme (ACE) Inhibitory Activity of Peptide Fraction of Germinated Pigeon Pea ( Cajanus cajan  (L.) Millsp.)

Ketut Ratnayani; I Ketut Suter; Nyoman Semadi Antara; I Nengah Kencana Putra

doi:10.22146/ijc.37513

Angiotensin Converting Enzyme (ACE) Inhibitory Activity of Peptide Fraction of Germinated Pigeon Pea (Cajanus cajan (L.) Millsp.)

https://doi.org/10.22146/ijc.37513

Ketut Ratnayani^(1*), I Ketut Suter⁽²⁾, Nyoman Semadi Antara⁽³⁾, I Nengah Kencana Putra⁽⁴⁾

(1) Department of Chemistry, Faculty of Natural Sciences, Udayana University, Denpasar 80361, Indonesia
(2) Department of Food Science and Technology, Faculty of Agricultural Technology, Udayana University, Denpasar 80361, Indonesia
(3) Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University, Denpasar 80361, Indonesia
(4) Department of Food Science and Technology, Faculty of Agricultural Technology, Udayana University, Denpasar 80361, Indonesia
(*) Corresponding Author

Abstract

During the germination process, seeds can release various types of peptides due to the degradation of storage proteins. Some of these peptides can have biological activity (bioactive peptides). The objective of this study was to determine the ACE inhibitory activity of germinated pigeon pea peptide extract at various germination times and to carry out the fractionation to the extract to get the most active peptide fraction. The results showed that the highest activity of peptide extract was found on the 4^th-day germination of pigeon pea with an IC₅₀ value of 63.46 μg/mL. The peptide extract was further fractionated by centrifugal ultrafiltration method and it was found that the peptide fraction < 3 kDa had the highest ACE inhibitory activity with an IC₅₀ value of 57.79 μg/mL. The result of identification with the LCMS method to the fraction was able to detect 4 types of the peptide with a molecular weight of 230.304, 294.303, 441.436, and 570.591 Da. These results suggested that the peptide fraction of germinated pigeon pea has the potency as an ACE inhibitory nutraceutical.

Keywords

pigeon pea; peptide fraction; ACE inhibitory; germination

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DOI: https://doi.org/10.22146/ijc.37513