Pyrolusite Bioleaching by an Indigenous Acidithiobacillus sp KL3 Isolated from an Indonesian Sulfurous River Sediment

https://doi.org/10.22146/ijc.38898

Endah Retnaningrum(1*), Wahyu Wilopo(2)

(1) Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan Sekip Utara, Yogyakarta 55281, Indonesia
(2) Geological Engineering Department, Faculty of Engineering, Universitas Gadjah Mada, Jl. Grafika No. 2, Bulaksumur, Yogyakarta 55281, Indonesia
(*) Corresponding Author

Abstract


The manganese bioleaching process of pyrolusite from Kliripan, Indonesia using Acidithiobacillus sp KL3 was investigated. The influence pulp densities of pyrolusite (0.01, 0.02, 0.03 and 0.05 g/cm3) on the bioleaching processes were studied for 16 days. The reduction on pH values, the increasing of oxidation-reduction potential (ORP), sulfate and manganese concentration were analyzed. The manganese bioleaching mechanism of pyrolusite by the strain was monitored using Scanning Electron Microscope-Energy Dispersive-X-ray Spectroscopy (SEM-EDX). The results indicated that 0.02 g/cm3 of pyrolusite was considered to be the optimal pulp density for manganese bioleaching process. During this process, pH values decreased, furthermore resulted in increasing of ORP, the concentration of sulfate and manganese. SEM-EDX analysis clearly showed the evidence of directly bacterial cell attachment into the surface of pyrolusite. Extracellular polymeric substances (EPSs) were further founded on that surface. Sulfur elemental was oxidized by the strain which was then confirmed of resulting in solubilized manganese.

Keywords


substrate; oxidation-reduction potential; bacterial cell attachment; sulfur elemental; solubilized manganese

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References

[1] Hagelstein, K., 2009, Globally sustainable manganese metal production and use, J. Environ. Manage., 90 (12), 3736–3740.

[2] Das, A.P., Ghosh, S., Mohanty, S., and Sukla, L.B., 2014, Consequences of manganese compounds: A review, Toxicol. Environ. Chem., 96 (7), 981–997.

[3] Bayard, R., Chatain, V., Gachet, C., Troadec, A., and Gourdon, R., 2006, Mobilisation of arsenic from a mining soil in batch slurry experiments under bio-oxidative conditions, Water Res., 40 (6), 1240–1248.

[4] Nguyen V.K., and Lee, J.U., 2015, A comparison of microbial leaching and chemical leaching of arsenic and heavy metals from mine tailings, Biotechnol. Bioprocess Eng., 20 (1), 91–99.

[5] Ghosh, S., Mohanty, S., Akcil, A., Sukla, L.B., and Das, A.P., 2016, A greener approach for resource recycling: Manganese bioleaching, Chemosphere, 154, 628–639.

[6] Pathak, A., Morrison, L., and Healy, M.G., 2017, Catalytic potential of selected metal ions for bioleaching, and potential techno-economic and environmental issues: A critical review, Bioresour. Technol., 229, 211–221.

[7] Ramdhani, E.P., Wahyuni, T., Ni’mah, Y.L., Suprapto, and Prasetyoko, D., 2018, Extraction of alumina from red mud for synthesis of mesoporous alumina by adding CTABr as mesoporous directing agent, Indones. J. Chem., 18 (2), 337–343.

[8] Bhushan, B., Nayak, A., and Kamaluddin, 2017, Role of manganese oxides in peptide synthesis: implication in chemical evolution, Int. J. Astrobiol., 16 (4), 360–367.

[9] Schaefer, M.V., Handler, R.M., and Scherer, M.M., 2017, Fe(II) reduction of pyrolusite (β-MnO2) and secondary mineral evolution, Geochem. Trans., 18 (1), 7.

[10] Pokorna, D., and Zabranska, J., 2015, Sulfur-oxidizing bacteria in environmental technology, Biotechnol. Adv., 33 (6), 1246–1259.

[11] Jeremic, S., Beškoski, V.P., Djokic, L., Branka, V., Vrvić, M.M., Avdalović, J., Cvijović, G.G., Beškoski, L.S., and Nikodinovic-Runic, J., 2016, Interactions of the metal tolerant heterotrophic microorganisms and iron oxidizing autotrophic bacteria from sulphidic mine environment during bioleaching experiments, J. Environ. Manage., 172, 151–161.

[12] Cho, K.H., Kim, B.J., Choi, N.C., Kim, S.B., and Park, C.Y., 2012, Bioleaching of chalcopyrite using indigenous acidophilic bacteria under moderate thermopile conditions, Geosyst. Eng., 15 (4), 229–238.

[13] Shiers, D.W., Ralph, D.E., Bryan, C.G., and Watling, H.R., 2013, Substrate utilisation by Acidianus brierleyi, Metallosphaera hakonensis and Sulfolobus metallicus in mixed ferrous ion and tetrathionate growth media, Miner. Eng., 48, 86–93.

[14] Liu, H.C., Nie, Z.Y., Xia, J.L., Zhu, H.R., Yang, Y., Zhao, C.H., Zheng, L., and Zhao, Y.D., 2015, Investigation of copper, iron and sulfur speciation during bioleaching of chalcopyrite by moderate thermophile Sulfobacillus thermosulfidooxidans, Int. J. Miner. Process., 137, 1–8.

[15] Kim, I.S., Lee, J.U., and Jang, A., 2005, Bioleaching of heavy metals from dewatered sludge by Acidithiobacillus ferrooxidans, J. Chem. Technol. Biotechnol., 80 (12), 1339–1348.

[16] Sarcheshmehpour, Z., Lakzian, A., Fotovat, A., Berenji, A.R., Haghnia, G.H., and Seyed Bagheri, S.A., 2009, Possibility of using chemical fertilizers instead of 9K medium in bioleaching process of low-grade sulfide copper ores, Hydrometallurgy, 96, 264–267.

[17] Gerayeli, F., Ghojavand, F., Mousavi, S.M., Yaghmaei, S., and Amiri, F., 2013, Screening and optimization of effective parameters in biological extraction of heavy metals from refinery spent catalysts using a thermophilic bacterium, Sep. Purif. Technol., 118, 151–161.

[18] Kolmert, Å., Wikström, P., and Hallberg, K.B., 2000, A fast and simple turbidimetric method for the determination of sulfate in sulfate-reducing bacterial cultures, J. Microbiol. Methods, 41 (3), 179–184.

[19] Khayatian, G., Moradi, M., and Hassanpoor, S., 2018, MnO2/3MgO Nanocomposite for preconcentration and determination of trace copper and lead in food and water by flame atomic absorption spectrometry, J. Anal. Chem., 73 (5), 470–478.

[20] Cardell, C., and Guerra, I., 2015, An overview of emerging hyphenated SEM-EDX and Raman spectroscopy systems: Applications in life, environmental and materials sciences, TrAC, Trends Anal. Chem., 77, 156–166.

[21] Lombardi, A.T., Garcia, O., and Mozeto, A.A., 2001, Bioleaching of metals from anaerobic sewage sludge: Effects of total solids, leaching microorganisms, and energy source, J. Environ. Sci. Health. Part A Toxic/Hazard. Subst. Environ. Eng., 36 (5), 793–806.

[22] Foulkes, B., Khanal, S.K., and Sung, S., 2006, Bioleaching of zinc and copper from anaerobically digested swine manure: effect of sulfur levels and solids contents, Water Environ. Res., 78, 202–208.

[23] Chen S.Y., and Lin, J.G., 2001, Effect of substrate concentration on bioleaching of metal-contaminated sediment, J. Hazard. Mater, 82 (1), 77–89.

[24] Rojas-Chapana J.A., and Tributsch, H., 2004, Interfacial activity and leaching patterns of Leptospirillum ferrooxidans on pyrite, FEMS Microbiol. Ecol., 47 (1), 19–29.

[25] d’Hugues, P., Joulian, C., Spolaore, P., Michel, C., Garrido, F., and Morin, D., 2008, Continuous bioleaching of a pyrite concentrate in stirred reactors: Population dynamics and exopolysaccharide production vs. bioleaching performance, Hydrometallurgy, 94 (1-4), 34–41.

[26] Bellenberg, S., Díaz, M., Noël, N., Sand, W., Poetsch, A., Guiliani, N., and Vera, M., 2014, Biofilm formation, communication and interactions of leaching bacteria during colonization of pyrite and sulfur surfaces, Res. Microbiol., 165 (9), 773–781.

[27] Talla, E., Hedrich, S., Mangenot, S., Ji, B., Johnson, D.B., Barbe, V., and Bonnefoy, V., 2014, Insights into the pathways of iron- and sulfur-oxidation, and biofilm formation from the chemolithotrophic acidophile Acidithiobacillus ferrivorans CF27, Res. Microbiol., 165 (9), 753–760.

[28] Han, Y., Ma, X., Zhao, W., Chang, Y., Zhang, X., Wang, X., Wang, J., and Huang, Z., 2013, Sulfur oxidizing bacteria dominate the microbial diversity shift during the pyrite and low-grade pyrolusite bioleaching process, J. Biosci. Bioeng., 116 (4), 465–471.

[29] Sand, W., Gehrke, T., Jozsa, P.G., and Schippers, A., 2001, Biochemistry of bacterial leaching-direct vs. indirect bioleaching, Hydrometallurgy, 59 (2), 159–175.

[30] Fortin, D., and Langley, S., 2005, Formation and occurrence of biogenic iron-rich minerals, Earth Sci. Rev., 72 (1-2), 1–19.



DOI: https://doi.org/10.22146/ijc.38898

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