Physicochemical and Microbiological Analysis of Stingless Bees Honey Collected from Local Market in Malaysia

Wan Nadja Julika(1), Azilah Ajit(2*), Ahmad Ziad Sulaiman(3), Aishath Naila(4)

(1) Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia
(2) Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia
(3) Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia
(4) Maldives National University, Central Administration, Rehdebai Hingun, Machangolhi, Male’, Maldives
(*) Corresponding Author


The growing demand for honey in the market has led to the occurrence of the tampering honey with foreign substances and increases the production of artificial honey. Due to this concern, this study works on the physicochemical and microbial characterization of stingless bee honey. The physicochemical analysis showed that the honey possessed pH (2.51–3.26), free acidity (121.1 to 318.7 meq/kg), moisture (19.4–30.9%), electrical conductivity (0.33–0.69 mS/cm), ash content (2.75–4.31 g/100g), Hydroxymethylfurfural (HMF) content (35.4 to 461.7 mg/kg) and diastase activity (2.71 to 6.11 DN). Also, sugar profile of honey showed that the honey contained fructose (15.03–32.52 g/100g), glucose (12.17–34.55 g/100g) and sucrose (0.01–7.29 g/100g). The harvested honey, H1, and H2 have the highest potential to become an antibacterial agent to treat disease compared to commercial honey samples because they were active against Gram-negative bacteria. All analyzed samples were within the maximum limit of the quality criteria set by the Malaysian Kelulut Standard and Codex Alimentarius except for free acidity, HMF, and Diastase Number. All the data obtained is vital in order to create a specific statute for stingless bees honey in Malaysia that may help to protect the consumer from purchasing adulterated honey.


Honey; Adulteration; Physicochemical; Microbiological; Quality

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[1] Mathiasson, M.E., Kwapong, P.K., Wubah, D.A., and Wubah, J.A., 2015, Early colony development of an equatorial afrotropical stingless bee (Hypotrigona sp.) In a new habitat, J. Young Invest., 29 (3), 11–17.

[2] Rao, P.V., Krishnan, K.T., Salleh, N., and Gan, S.H., 2016, Biological and therapeutic effects of honey produced by honey bees and stingless bees: A comparative review, Braz. J. Pharmacogn., 26 (5), 657–664.

[3] Matović, K., Ćirić, J., Kaljević, V., Nedić, N., Jevtić, G., Vasković, N., and Baltić, M.Ž., 2018, Physicochemical parameters and microbiological status of honey produced in an urban environment in Serbia, Environ. Sci. Pollut. Res., 25 (14), 14148–14157.

[4] Nordin, A., Sainik, N.Q.A.V., Chowdhury, S.R., Bin Saim, A., and Bt Hj Idrus, R., 2018, Physicochemical properties of stingless bee honey from around the globe: A comprehensive review, J. Food Compos. Anal., 73, 91–102.

[5] Adenekan, M.O., Amusa, N.A, Lawal, A.O., and Okpeze, V.E., 2010, Physico-chemical and microbiological properties of honey samples obtained from Ibadan, J. Microbiol. Antimicrob., 2 (8), 100–104.

[6] Snowdon, J.A., and Cliver, D.O., 1996, Microorganisms in honey, Int. J. Food Microbiol., 31 (1-3), 1–26.

[7] Gomes, S., Dias, L.G., Moreira, L.L., Rodrigues, P., and Estevinho, L., 2010, Physicochemical, microbiological and antimicrobial properties of commercial honeys from Portugal, Food Chem. Toxicol., 48 (2), 544–548.

[8] Islam, M.N., Khalil, M.I., Islam, M.A., and Gan, S.H., 2014, Toxic compounds in honey, J. Appl. Toxicol., 34 (7), 733–742.

[9] Gan, Z., Yang, Y., Li, J., Wen, X., Zhu, M., Jiang, Y., and Ni, Y., 2015, Using sensor and spectral analysis to classify botanical origin and determine adulteration of raw honey, J. Food Eng., 178, 151–158.

[10] Serrano, S., Villarejo, M., Espejo, R., and Jodral, M., 2004, Chemical and physical parameters of Andalusian honey: Classification of Citrus and Eucalyptus honeys by discriminant analysis, Food Chem., 87 (4), 619–625.

[11] Bogdanov, S., 2009, Harmonised Methods of the International Honey Commision, Swiss Research Centre, FAM, Liebefeld.

[12] Malaysian Standard, 2017, Kelulut (Stingless bee) honey - Specification: MS 2683- 2017, Department of Standard Malaysia., 67.180.10.

[13] Moussa, A., Noureddine, D., Abdelmelek, M., and Saad, A., 2012, Antibacterial activity of various honey types of Algeria against Pathogenic Gram-Negative Bacilli: Escherichia coli and Pseudomonas aeruginosa, Asian Pac. J. Trop. Dis., 2 (3), 211–214.

[14] de Sousa, J.M.B., de Souza, E.L., Marques, G., Benassi, M.T., Gullón, B., Pintado, M.M., and Magnani, M., 2016, Sugar profile, physicochemical and sensory aspects of monofloral honeys produced by different stingless bee species in Brazilian semi-arid region, LWT Food Sci. Technol., 65, 645–651.

[15] Biluca, F.C., Braghini, F., Gonzaga, L.V., Costa, A.C.O., and Fett, R., 2016, Physicochemical profiles, minerals and bioactive compounds of stingless bee honey (Meliponinae), J. Food Compos. Anal., 50, 61–69.

[16] El Sohaimy, S.A., Masry, S.H.D., and Shehata, M.G., 2015, Physicochemical characteristics of honey from different origins, Ann. Agric. Sci., 60 (2), 279–287.

[17] Gangwar, S., 2016, Honey physio-chemical parameters and its application with reference to Ethiopia, IJSN, 7 (1), 16–24.

[18] Bogdanov, S., and Gallman, P., 2008, Authenticity of honey and other bee products state of the art, ALP Sci., 520, 63–64.

[19] Yadata, D., 2014, Detection of the electrical conductivity and acidity of honey from different areas of Tepi, Food Sci. Technol., 2 (5), 59–63.

[20] Taddia, M., Musiani, A., and Schiavi, S., 2004, Determination of heavy metals in honey by Zeeman electrothermal atomic absorption spectrometry, Ann. Chim., 94 (1‐2), 107–111.

[21] Malika, N., Mohamed, F., and Chakib, E., 2005, Microbiological and physico-chemical properties of Moroccan honey, Int. J. Agric. Biol, 7 (5), 773–776.

[22] Alqarni, A.S., Owayss, A.A., Mahmoud, A.A., and Hannan, M.A., 2014, Mineral content and physical properties of local and imported honeys in Saudi Arabia, J. Saudi Chem. Soc., 18 (5), 618–625.

[23] Önür, İ., Misra, N.N., Barba, F.J., Putnik, P., Lorenzo, J.M., Gökmen, V., and Alpas, H., 2018, Effects of ultrasound and high pressure on physicochemical properties and HMF formation in Turkish honey types, J. Food Eng., 219, 129–136.

[24] Finola, M.S., Lasagno, M.C., and Marioli, J.M., 2007, Microbiological and chemical characterization of honeys from central Argentina, Food Chem., 100 (4), 1649–1653.

[25] Andualem, B., 2014, Physico-chemical, microbiological and antibacterial properties of Apis mellipodae and Trigona spp. honey against bacterial pathogens, World J. Agric. Sci., 10 (3), 112–120.

[26] Zappalà, M., Fallico, B., Arena, E., and Verzera, A., 2005, Methods for the determination of HMF in honey: A comparison, Food Control, 16 (3), 273–277.

[27] Capuano, E., and Fogliano, V., 2011, Acrylamide and 5-hydroxymethylfurfural (HMF): A review on metabolism, toxicity, occurrence in food and mitigation strategies, LWT Food Sci. Technol., 44 (4), 793–810.

[28] Yücel, Y., and Sultanoğlu, P., 2013, Characterization of honeys from Hatay Region by their physicochemical properties combined with chemometrics, Food Biosci., 1, 16–25.

[29] da Silva, P.M., Gauche, C., Gonzaga, L.V., Costa, A.C.O., and Fett, R., 2016, Honey: Chemical composition, stability and authenticity, Food Chem., 196, 309–323.

[30] Pasias, I.N., Kiriakou, I.K., and Proestos, C., 2017, HMF and diastase activity in honeys: A fully validated approach and a chemometric analysis for identification of honey freshness and adulteration, Food Chem., 229, 425–431.

[31] Buba, F., Gidado, A., and Shugaba, A., 2013, Physicochemical and microbiological properties of honey from North East Nigeria, Biochem. Anal. Biochem., 2, 142.

[32] Chuttong, B., Chanbang, Y., Sringarm, K., and Burgett, M., 2016, Physicochemical profiles of stingless bee (Apidae: Meliponini) honey from South East Asia (Thailand), Food Chem., 192, 149–155.

[33] Kek, S.P., Chin, N.L., Tan, S.W., Yusof, Y.A., and Chua, L.S., 2017, Classification of honey from its bee origin via chemical profiles and mineral content, Food Anal. Methods, 10 (1), 19–30.

[34] Anklam, E., 1998, A review of the analytical methods to determine the geographical and botanical origin of honey, Food Chem., 63 (4), 549–562.

[35] Suntiparapop, K., Prapaipong, P., and Chantawannakul, P., 2012, Chemical and biological properties of honey from Thai stingless bee (Tetragonula leaviceps), J. Apic. Res., 51 (1), 45–52.

[36] Iftikhar, F., Mahmood, R., Islam, N., Sarwar, G., Masood, M.A., and Shafiq, H., 2014, Physicochemical analysis of honey samples collected from local markets of Rawalpindi and Islamabad, Am. J. Biochem., 4 (2), 35–40.

[37] Pucciarelli, A.B., Schapovaloff, M.E., Kummritz, S., Señuk, I.A., Brumovsky, L.A., and Dallagnol, A.M., 2014, Microbiological and physicochemical analysis of yateí (Tetragonisca angustula) honey for assessing quality standards and commercialization, Rev. Argent. Microbiol., 46 (4), 325–332.

[38] Agbagwa, O.E., Otokunefor, T.V, and Frank-Peterside, N., 2014, Preliminary detection of Bacillus species in commercial honey, Br. Microbiol. Res. J., 4 (12), 1370–1380.

[39] Yaacob, M., Rajab, N.F., Shahar, S., and Sharif, R., 2017, Stingless bee honey and its potential value: a systematic review, Food Res., 2 (2), 124–133.


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