Antibacterial Activity of Silver Nanoparticles Capped by p-Aminobenzoic Acid on Escherichia coli and Staphylococcus aureus

Dian Susanthy(1*), Sri Juari Santosa(2), Eko Sri Kunarti(3)

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara BLS 21, Bulaksumur, Yogyakarta 55281, Indonesia
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara BLS 21, Bulaksumur, Yogyakarta 55281, Indonesia
(3) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara BLS 21, Bulaksumur, Yogyakarta 55281, Indonesia
(*) Corresponding Author


This paper describes the antibacterial performance of silver nanoparticles (AgNPs) which have been synthesized by using p-aminobenzoic acid as reducing and stabilizing agent simultaneously. The silver nitrate with various concentrations was reacted with pH 11-adjusted p-aminobenzoic acid with a concentration of 5 × 10–3 mol L–1 for 30 min in a boiling water bath. The synthesized AgNPs were characterized by UV-Vis spectrophotometry, Transmission Electron Microscope (TEM), and Particle Size Analyzer (PSA). The antibacterial performance of the synthesized AgNPs was evaluated by agar well diffusion method on Escherichia coli and Staphylococcus aureus. The higher silver nitrate concentration, the bigger the nanoparticle size, the wider particle size distribution, and the higher number of AgNPs formed. AgNPs synthesized from higher silver nitrate concentration had higher antibacterial activity. It is an indication that the antibacterial activity of AgNPs is mainly controlled by the silver ion concentration which influences the AgNPs particle size and existence of silver ion in the AgNPs colloidal solution


antibacterial activity; p-aminobenzoic acid; silver nanoparticles

Full Text:

Full Text PDF


[1] Abbasi, E., Milani, M., Aval, S.F., Kouhi, M., Akbarzadeh, A., Nasrabadi, H.T., Nikasa, P., Joo, S.W., Hanifehpour, Y., Nejati-Koshki, K., and Samiei, M., 2016, Silver nanoparticles: Synthesis methods, bio-applications and properties, Crit. Rev. Microbiol., 42 (2), 173–180.

[2] Kędziora, A., Speruda, M., Krzyżewska, E., Rybka, J., Łukowiak, A., and Bugla-Płoskońska, G., 2018, Similarities and differences between silver ions and silver in nanoforms as antibacterial agents, Int. J. Mol. Sci., 19 (2), 444.

[3] Marambio-Jones, C., and Hoek, E.M.V, 2010, A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment, J. Nanopart. Res., 12 (5), 1531–1551.

[4] Zhou, L., Ji, Y., Zeng, C., Zhang, Y., Wang, Z., and Yang, X., 2013, Aquatic photodegradation of sunscreen agent p-aminobenzoic acid in the presence of dissolved organic matter, Water Res., 47 (1), 153–162.

[5] Cobley, C.M., Skrabalak, S.E., Campbell, D.J., and Xia, Y., 2009, Shape-controlled synthesis of silver nanoparticles for plasmonic and sensing applications, Plasmonics, 4 (2), 171–179.

[6] Bhatte, K.D., Tambade, P.J., Dhake, K.P., and Bhanage, B.M., 2010, Silver nanoparticles as an efficient, heterogeneous and recyclable catalyst for synthesis of β-enaminones, Catal. Commun., 11 (15), 1233–1237.

[7] Duan, H., Wang, D., and Li, Y., 2015, Green chemistry for nanoparticle synthesis, Chem. Soc. Rev., 44 (16), 5778–5792.

[8] Kim, J.S., Kuk, E., Yu, K.N., Kim, J.H., Park, S.J., Lee, H.J., Kim, S.H., Park, Y.K., Park, Y.H., Hwang, C.Y., Kim, Y.K., Lee, Y.S., Jeong, D.H., and Cho, M.H., 2007, Antimicrobial effects of silver nanoparticles, Nanomed. Nanotechnol. Biol. Med., 3 (1), 95–101.

[9] Kumar-Krishnan, S., Prokhorov, E., Hernández-Iturriaga, M., Mota-Morales, J.D., Vázquez-Lepe, M., Kovalenko, Y., Sanchez, I.C., and Luna-Bárcenas, G., 2015, Chitosan/silver nanocomposites: Synergistic antibacterial action of silver nanoparticles and silver ions, Eur. Polym. J., 67, 242–251.

[10] Veerasamy, R., Xin, T.Z., Gunasagaran, S., Xiang, T.F.W., Yang, E.F.C., Jeyakumar, N., and Dhanaraj, S.A., 2011, Biosynthesis of silver nanoparticles using mangosteen leaf extract and evaluation of their antimicrobial activities, J. Saudi Chem. Soc., 15 (2), 113–120.

[11] Susanthy, D., Santosa, S.J., and Kunarti, E.S., 2018, The synthesis and stability study of silver nanoparticles prepared using p-aminobenzoic acid as reducing and stabilizing agent, Indones. J. Chem., 18 (3), 421–427.

[12] Roto, R., Marcelina, M., Aprilita, N.H., Mudasir, M., Natsir, T.A., and Mellisani, B., 2017, Investigation on the effect of addition of Fe3+ ion into the colloidal AgNPs in PVA solution and understanding its reaction mechanism, Indones. J. Chem., 17 (3), 439–445.

[13] Roto, R., Rasydta, H.P., Suratman, A., and Aprilita, N.H., 2018, Effect of reducing agents on physical and chemical properties of silver nanoparticles, Indones. J. Chem., 18 (4), 614–620.

[14] Wanger, A., 2007, “Disk diffusion tests and gradient methodologies” in Antimicrobial Susceptibility Testing Protocols, 1st Ed., Eds. Schwalbe, R., Steele-Moore, L., and Goodwin, A.C., CRC Press, Boca Raton, 53–73.

[15] CLSI, 2016, Performance standards for antimicrobial susceptibility testing, CLSI supplement M100S, 26th Ed., Clinical and Laboratory Standards Institute, Wayne, Philadelphia, USA.

[16] Vasileva, P., Donkova, B., Karadjova, I., and Dushkin, C., 2011, Synthesis of starch-stabilized silver nanoparticles and their application as a surface plasmon resonance-based sensor of hydrogen peroxide, Colloids Surf., A, 382 (1-3), 203–210.

[17] Chhatre, A., Solasa, P., Sakle, S., Thaokar, R., and Mehra, A., 2012, Color and surface plasmon effects in nanoparticle systems: Case of silver nanoparticles prepared by microemulsion route, Colloids Surf., A, 404, 83–92.

[18] Ratnarathorn, N., Chailapakul, O., Henry, C.S., and Dungchai, W., 2012, Simple silver nanoparticle colorimetric sensing for copper by paper-based devices, Talanta, 99, 552–557.

[19] Susilowati, E., Triyono, Santosa, S.J., and Kartini, I., 2015, Synthesis of silver-chitosan nanocomposites colloidal by glucose as reducing agent, Indones. J. Chem., 15 (1), 29–35.

[20] Daniel, S.C.G.K., Julius, L.A.N., and Gorthi, S.S., 2017, Instantaneous detection of melamine by interference biosynthesis of silver nanoparticles, Sens. Actuators, B, 238, 641–650.

[21] Paramelle, D., Sadovoy, A., Gorelik, S., Free, P., Hobley, J., and Fernig, D.G., 2014, A rapid method to estimate the concentration of citrate capped silver nanoparticles from UV-visible light spectra, Analyst, 139 (19), 4855–4861.

[22] Chen, K., Shen, Z., Luo, J., Wang, X., and Sun, R., 2015, Quaternized chitosan/silver nanoparticles composite as a SERS substrate for detecting tricyclazole and Sudan I, Appl. Surf. Sci., 351, 466–473.

[23] Hebeish, A.A., El-Rafie, M.H., Abdel-Mohdy, F.A., Abdel-Halim, E.S., and Emam, H.E., 2010, Carboxymethyl cellulose for green synthesis and stabilization of silver nanoparticles, Carbohydr. Polym., 82 (3), 933–941.

[24] Gusrizal, G., Santosa, S.J., Kunarti, E.S., and Rusdiarso, B., 2016, Dual function of p-hydroxybenzoic acid as reducing and capping agent in rapid and simple formation of stable silver nanoparticles, Int. J. ChemTech Res., 9 (9), 472–482.

[25] Song, K.C., Lee, S.M., Park, T.S., and Lee, B.S., 2009, Preparation of colloidal silver nanoparticles by chemical reduction method, Korean J. Chem. Eng., 26 (1), 153–155.

[26] Šileikaite, A., Puišo, J., Prosyčevas, I., and Tamulevičius, S., 2009, Investigation of silver nanoparticles formation kinetics during reduction of silver nitrate with sodium citrate, Mater. Sci., 15 (1), 21–27.

[27] Pandey, S., Goswami, G.K., and Nanda, K.K., 2012, Green synthesis of biopolymer–silver nanoparticle nanocomposite: An optical sensor for ammonia detection, Int. J. Biol. Macromol., 51 (4), 583–589.

[28] Mittal, A.K., Chisti, Y., and Banerjee, U.C., 2013, Synthesis of metallic nanoparticles using plant extracts, Biotechnol. Adv., 31 (2), 346–356.

[29] Tran, H.V., Tran, L.D., Ba, C.T., Vu, H.D., Nguyen, T.N., Pham, D.G., and Nguyen, P.X., 2010, Synthesis, characterization, antibacterial and antiproliferative activities of monodisperse chitosan-based silver nanoparticles, Colloids Surf., A, 360 (1-3), 32–40.

[30] Prabhu, S., and Poulose, E.K., 2012, Silver nanoparticles: Mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects, Int. Nano Lett., 2, 32.

[31] Guzman, M.G., Dille, J., and Godet, S., 2009, Synthesis of silver nanoparticles by chemical reduction method and their antibacterial activity, Int. J. Chem. Biomol. Eng., 2 (3), 104–111.

[32] Wang, W., Hong, M.C., Luo, J., Jiang, F., Han, L., Lin, Z., and Cao, R., 2004, Syntheses and characterizations of six hydrogen-bonded silver(I) complexes from assembly of silver(I) nitrate and aminobenzoic acid, Inorg. Chim. Acta, 357, 103–114.

[33] Hassabo, A.G., Nada, A.A., Ibrahim, H.M., and Abou-Zeid, N.Y., 2015, Impregnation of silver nanoparticles into polysaccharide substrates and their properties, Carbohydr. Polym., 122, 343–350.


Article Metrics

Abstract views : 6368 | views : 2325

Copyright (c) 2019 Indonesian Journal of Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.


Indonesian Journal of Chemistry (ISSN 1411-9420 /e-ISSN 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

Analytics View The Statistics of Indones. J. Chem.