Selective Solid-Phase Extraction of Meropenem from Human Blood Plasma Using a Molecularly Imprinted Polymer

Lasmaryna Sirumapea(1*), Mohammad Ali Zulfikar(2), Muhammad Bachri Amran(3), Anita Alni(4)

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bandung Institute of Technology, Jl. Ganesha No. 10, Bandung 40132, West Java, Indonesia; STIFI Bhakti Pertiwi Palembang, Jl. Ariodillah III No. 22, Palembang 30128, South Sumatera, Indonesia
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bandung Institute of Technology, Jl. Ganesha No. 10, Bandung 40132, West Java, Indonesia
(3) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bandung Institute of Technology, Jl. Ganesha No. 10, Bandung 40132, West Java, Indonesia
(4) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bandung Institute of Technology, Jl. Ganesha No. 10, Bandung 40132, West Java, Indonesia
(*) Corresponding Author


This study employed a selective and high adsorption performance for meropenem. Molecularly imprinted polymer for meropenem (MeIP) as the selective sorbent was prepared through a bulk polymerization reaction. Methacrylic acid, ethylene glycol dimethacrylate, benzoyl peroxide, and dimethyl sulfoxide were applied as functional monomer, crosslinker agent, initiator, and solvent, respectively. Scanning electron microscopy, thermogravimetric analysis, Brunauer-Emmett-Teller analysis, and Fourier transform infrared spectroscopy were used to characterize the morphology, pore size, and structure of imprinted polymers. The maximum adsorption capacity was achieved at pH = 3, after 4 h contacted, under 150 rpm, and 25 mg of polymer applied. The maximum adsorption capacity of MeIP for meropenem was 51.963 mg/L; the synthesized polymer had superior selectivity to meropenem compared to that of the other antibiotics (imprinting factor, IF = 2.58). Furthermore, the thermodynamic and kinetic analyses indicated that the results were in accord with the Freundlich model and the pseudo-second-order kinetic model, respectively. MeIP was selective in batch adsorption, and molecularly imprinted solid-phase extraction protocols were selective to meropenem. It was then applied to analyze meropenem in human blood plasma and resulted in 78.52 ± 2.71 of recovery.


meropenem; molecularly imprinted polymer; selective; solid-phase extraction

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